Inhibition effect of epicatechin gallate on acid phosphatases from rainbow trout (Oncorhynchus mykiss) liver by multispectral and molecular docking.

Inhibition of acid phosphatase, which significantly contributes to inosine 5'-monophosphate (IMP) degradation, is crucial for preventing flavor deterioration of aquatic products during storage. In this study, the inhibitory effect of epicatechin gallate (ECG) on the activity of acid phosphatase isozymes (ACPI and ACPII) was analyzed using inhibition kinetics, fluorescence spectroscopy, isothermal titration calorimetry, and molecular simulation. ACPI and ACPII with molecular weights of 59.

Sensitive and high resolution subcutaneous fluorescence in vivo imaging using upconversion nanoparticles and microarrays.

A sensitive and high resolution small animal in vivo imaging system using upconversion nanoparticles (UNPs) and microarrays was developed. The fluorescence tomography using UNPs could achieve higher precision than that using ordinary fluorophores, which was theoretically explained by the finite element method (FEM). Given the autofluorescence-insensitive property of UNPs, a high subcutaneous detection sensitivity of 0.

Use of hyperspectral imaging for label-free decoding and detection of biomarkers.

Detecting the existence of biomarkers is one of the most important issues in molecular diagnosis. This Letter presents a label-free decoding and detection method for biomarker tests based on hyperspectral imaging and interferometry. The decoding and detection results were extracted from a single hyperspectral image with different spectrum-to-thickness reconstructing algorithms, which made the testing procedure extremely fast and simple.

Rapid, on-site identification of explosives in nanoliter droplets using a UV reflected fiber optic sensor.

A portable UV (190-400 nm) spectrophotometric based reflected fiber optic sensor system is presented for the on-site detection and identification of explosives. A reflected fiber optic sensor for explosives analysis was developed, with low sample consumption (20-100 nL) and a wide concentration quantification range (1.1-250 mg L(-1)).