Publications by authors named "Songgang Wu"
L-valine is an essential branched-amino acid that is widely used in multiple areas such as pharmaceuticals and special dietary products and its use is increasing. As the world market for L-valine grows rapidly, there is an increasing interest to develop an efficient L-valine-producing strain. In this study, a simple, sensitive, efficient, and consistent screening procedure termed 96 well plate-PC-HPLC (96-PH) was developed for the rapid identification of high-yield L-valine strains to replace the traditional L-valine assay.
View Article and Find Full Text PDFA lipase gene (atl) was cloned from Aspergillus tamarii FS132 for the first time. The gene was found to have an open reading frame of 1024 base pairs (bp), and the coding region of the gene contained two introns (51 bp and 52 bp). Multi-alignment analysis of the deduced amino acid sequence indicated high homology between the enzyme and mono-and diacylglycerol lipases from fungi Aspergillus.
View Article and Find Full Text PDF[Overexpression of Penicillium expansum lipase gene in Pichia pastoris].
Sheng Wu Gong Cheng Xue Bao
March 2003
Article Synopsis
- The alkaline lipase gene from Penicillium expansum was successfully cloned into a yeast plasmid and transformed into a His4 mutant yeast strain, leading to the creation of recombinant Pichia strains confirmed by PCR.
- The overexpression of the PEL gene resulted in a functional recombinant lipase with a molecular mass of 28kD that effectively hydrolyzed olive oil, demonstrating its activity through clear halos in olive oil plates.
- Optimization of cultivation conditions, such as media composition and pH, significantly increased lipase production, with the absence of biotin and yeast nitrogen base enhancing yield by improving the pH stability of the enzyme.