Two new flavonoid glucosides from Pursh.

Two new flavonoids, Alpinetin-7-O-[3''--galloyl-4'',6''-hexahydroxy-diphenoyl]---glucose (), Pinocembrin-7--[4''--galloyl]---glucose (), and three known compound () were isolated from Pursh (Saxifragaceae). The structures of all compounds were deduced from their comprehensive spectroscopic analysis including IR, HR-ESI-MS,H NMR,C NMR, DEPT, COSY, HMBC and HMQC. Molecular docking model was used to test the anti-cholestatic liver activities of the isolated compounds, and compounds and showed higher docking scores (-9.

Construction of lignan glycosides biosynthetic network in Escherichia coli using mutltienzyme modules.

Background: Due to the complexity of the metabolic pathway network of active ingredients, precise targeted synthesis of any active ingredient on a synthetic network is a huge challenge. Based on a complete analysis of the active ingredient pathway in a species, this goal can be achieved by elucidating the functional differences of each enzyme in the pathway and achieving this goal through different combinations. Lignans are a class of phytoestrogens that are present abundantly in plants and play a role in various physiological activities of plants due to their structural diversity.