Purpose: To perceive the dental undergraduate's policy of coping with online learning and their decision-making laws during the COVID-19 pandemic.
Methods: For dental undergraduate students from the 2016 grade to 2018 grade of Lishui University, two prospective questionnaire surveys were conducted before the online course starting and four weeks later. SPSS Modeler18.
Objective: To clone, express, purify the Per a 4 gene encoding an allergen of and prepare monoclonal antibodies against the recombinant allergen.
Methods: The total RNA was extracted from , and the target gene was amplified by RT-PCR and cloned into pMD18-T vector. After being confirmed by nucleotide sequencing, the gene was then inserted into pGEX-3X to construct the express vector pGEX-3X-Per a 4.
Background: In this study, we screened and identified an endophyte JG09 having strong biocatalytic activity for ginsenosides from , converted ginseng total saponins and ginsenoside monomers, determined the source of minor ginsenosides and the transformation pathways, and calculated the maximum production of minor ginsenosides for the conversion of ginsenoside Rb1 to assess the transformation activity of endophyte JG09.
Methods: The transformation of ginseng total saponins and ginsenoside monomers Rb1, Rb2, Rc, Rd, Rg1 into minor ginsenosides F2, C-K and Rh1 using endophyte JG09 isolated by an organizational separation method and Esculin-R2A agar assay, as well as the identification of transformed products via TLC and HPLC, were evaluated. Endophyte JG09 was identified through DNA sequencing and phylogenetic analysis.
Objective: Less fungicides could be used to biocontrol Alternaria panax and Botrytis cinerea, this experiment can offer preliminary theory for wood vinegar as a botanic fungicide.
Methods: The in vitro inhibition activities of wood vinegar on Alternaria panax and Botrytis cinerea were tested by using mycelial growth rate method and spore germination method.
Results: Inhibition of mycelium growth rate to Alternaria panax was 100% when the concentration of wood vinegar was no less than 3.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
February 2014
Objective: To clone and express transferrin (Tsf) from Culex pipiens pallens in Pichia pastoris, and detect its antibacterial activity.
Methods: The coding region of transferrin from Culex pipiens pallens was amplified by RT-PCR. The product of RT-PCR was inserted into the downstream of gene encoding a-factor signal sequence in a Pichia pastoris secreting expression vector pGAPZalpha-A.
Objective: To study the effect of microRNA-21 (miRNA-21) on the regulation of the interleukin 12 (IL-12)/signal transducer and activator of transcription 4 (STAT4) pathway in the lung tissue of asthmatic mice.
Material And Methods: Forty five male C57BL/6 mice were randomly divided into three groups of 15 mice each: normal control, asthmatic model, and dexamethasone. Our mouse model of allergic asathma was established using OVA sensitization and challenge.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
February 2013
The gene-coding mature apyrase protein from Aedes albopictus was amplified by RT-PCR and cloned in frame with the a-factor secretion signal peptide into Pichia pastoris secreting expression vector pGAPZalpha-A resulting in the pGAPZa-A-apyrase. After being linearized by Bln I restriction enzyme, the recombinant pGAPZalpha-A-apyrase was trans-formed into Pichia pastoris GS115 by electroporation. Recombinant strains pGAPZalpha-A-apyrase/GS115 were screened on YPDS plates containing Zeocin and identified by PCR.
View Article and Find Full Text PDFTo study the association of polymorphisms of the IL-18 promoter (-607C/A and -137G/C) with bronchial asthma, 120 subjects with bronchial asthma were selected as the experimental group (49 cases with bronchial asthma and allergic rhinitis as experimental group 1 and 71 cases with bronchial asthma without allergic rhinitis as experimental group 2) and 120 healthy individuals were selected as the control group. A polymerase chain reaction sequence-specific primer (PCR-SSP) was used to identify the genotypic polymorphisms between the experimental and control groups. The results revealed that the frequencies of the CC, CA and AA genotypes at -607C/A in experimental group 1 were 18.
View Article and Find Full Text PDFThe objective of this study was to evaluate the possible association between the CD14-159 polymorphism and adult asthma in the Chinese population. A total of 188 asthmatic patients and 60 healthy adults were enrolled in the present study, and the CD14-159 polymorphism was genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR‑RFLP) analysis. The results showed that the frequencies of CC, CT and TT genotypes were 12.
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