Effect of Fructus Psoraleae on motility of gallbladder isolated smooth muscle strips from guinea pigs.

Aim: To observe the effect of Fructus Psoraleae on motility of isolated gallbladder muscle strips of guinea pigs and its mechanism.

Methods: Guinea pigs were hit to lose consciousness and the whole gallbladder was removed quickly. Two or three smooth muscle strips (8 mm x 3 mm) were cut along a longitudinal direction.

Proanthocyanidin from grape seeds potentiates anti-tumor activity of doxorubicin via immunomodulatory mechanism.

The purpose of this study was to investigate the anti-tumor activities of proanthocyanidin (PA) from grape seeds and doxorubicin (DOX) in vitro as well as in vivo, either alone or in combination and to explore the immunomodulatory mechanism in tumor-bearing mice. PA (12.5 approximately 200 mg/l) or DOX (0.

Effects of rhubarb on isolated gastric muscle strips of guinea pigs.

Aim: To study the effects of rhubarb (dried root of Rheum officinale Baill.) on contractile activity of isolated gastric muscle strips of guinea pigs and its possible mechanism.

Methods: A total of 48 guinea pigs were killed to remove the whole stomach.

Effect of rhubarb on contractile response of gallbladder smooth muscle strips isolated from guinea pigs.

Aim: To investigate the effect of rhubarb on contractile response of isolated gallbladder muscle strips from guinea pigs and its mechanism.

Methods: Guinea pigs were killed to remove the whole gallbladder. Two or three smooth muscle strips (8 mm x 3 mm) were cut along the longitudinal direction.

Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo.

Aim: To determine the mechanisms of effects of phytoestrogen genistein on the contracted rabbit aortic arteries in vitro, and observe the effect of genistein and 17-beta estradiol on mean arterial pressure (MAP) in ovariectomized (OVX) rats.

Methods: (1) Strips of rabbit aortic smooth muscle were suspended in organ baths containing Kreb's solution, and then isometric tension was measured. (2) Female mature Wistar rats underwent a bilateral ovariectomy (OVX).

Action of progesterone on contractile activity of isolated gastric strips in rats.

Aim: To study the effect of progesterone on contractile activity of isolated gastric strips in rats.

Methods: Wistar rats were sacrificed to remove whole stomach. Then, the stomach was opened and the mucosal layer was removed.

Effect of areca on contraction of colonic muscle strips in rats.

Aim: To investigate the effects of areca on the contractile activity of isolated colonic muscle strips in rats and mechanism involved.

Methods: Each strip (LMPC, longitudinal muscle of proximal colon; CMPC, circular muscle of proximal colon; LMDC, longitudinal muscle of distal colon; CMDC, circular muscle of distal colon.) was suspended in a tissue chamber containing 5 mL Krebs solution (37 degrees C), bubbled continuously with 950 mL.

Inhibition of beta-estradiol on trachea smooth muscle contraction in vitro and in vivo.

Aim: To investigate the effect of beta-estradiol on trachea smooth muscle contraction in vitro and in vivo.

Methods: (1) Rabbit tracheas were incubated in organ baths filled with Krebs solution and supplied with a mixed gas of 95 % O2 and 5 % CO2. The isometric force was measured by ink-writing recorders.

Effect of cholecystokinin and secretin on contractile activity of isolated gastric muscle strips in guinea pigs.

AIM:To study the effect of cholecystokinin-octapeptide (CCK-8) and secretin on contractile activity of isolated gastric muscle strips in guinea pigs.METHODS:Each isolated gastric muscle strip was suspended in a tissue chamber containing 5mL Krebs solution constantly warmed by water jacked at 37° and supplied with a mixed gas of 95% O(2) and 5% CO(2). After incubating for 1h under 1g tension, varied concentrations of CCK-8 and secretin were added respectively in the tissue chamber and the contractile response was measured isometrically on ink-writing recorders.

Effects of Dangshen on isolated gastric muscle strips in rats.

AIM:To study the effects of Dangshen dried root of Codonopsis Pilosula (Franch) Nannf on contractile activity of isolated gastric muscle strips in rats and its possible mechanism involved.METHODS:Each isolated gastric muscle strip was put in a tissue chamber containing 5ml Krebs solution, constantly warmed by water jacket at 37?mgr; and supplied with a mixed gas of 95% O(2) and 5% CO(2). After incubating for 1h with 1g tension, Dangshen of varied concentration was added cumulatively in the tissue chamber at intervals of 2 minutes.