The study focused on accurately measuring 5-methyltetrahydrofolate (5-Me-THF), a crucial form of folate in human blood, using advanced techniques like isotope dilution ultra-high performance liquid chromatography-mass spectrometry (ID-UPLC-MS).
Researchers validated their method by testing it on human plasma samples, ensuring it was repeatable and reliable across various sample sizes, including very small volumes down to 10 μL.
The successful application of this method for different volumes demonstrates its potential for providing precise quantification of 5-Me-THF in blood, which is important for health assessments.