Sichuan Da Xue Xue Bao Yi Xue Ban
March 2006
Objective: To elucidate the biofilm structure of Actinomyces viscosus and the spatial distribution of exopolysaccharides in it.
Methods: The Actinomyces viscosus biofilm was made by allowing bacteria to attach to the cover glass surface. The biofilm structure and exopolysaccharides distribution at 24 hours were stained with Fluorescein, BODIPY and Calcofluor respectively and were visualized by confocal laser scanning microscopy (CLSM).