Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed.

Background: High Salinity is a major environmental stress influencing growth and development of rice. Comparative proteomic analysis of hybrid rice shoot proteins from Shanyou 10 seedlings, a salt-tolerant hybrid variety, and Liangyoupeijiu seedlings, a salt-sensitive hybrid variety, was performed to identify new components involved in salt-stress signaling.

Results: Phenotypic analysis of one protein that was upregulated during salt-induced stress, cyclophilin 2 (OsCYP2), indicated that OsCYP2 transgenic rice seedlings had better tolerance to salt stress than did wild-type seedlings.

[Advances in plant proteomics. II. Application of proteome techniques to plant biology research].

Proteome techniques have widely been applied to the fields of plant genetics, plant development, and plant physiology and ecology to investigate plant genetic diversity, plant development such as seed maturation and germination processes, differentiation of plant tissue and organ, separation and functional identification of novel component of various organells, mechanisms of plant adapted to abiotic or biotic stresses including high temperature, low temperature, high salt, drought, and pathogens and insects, and interaction of plant with microbe. The prospects of plant proteomics are discussed.

[Advances in plant proteomics--I. Key techniques of proteome].

With the completion of genome sequences of the model plants, such as rice (Oryza sativa L.) and Arabidopsis thaliana, it has come into plant functional genomics era, which becomes a hard base of the appearance and development of plant proteomics. This review focuses on the background of proteomics, concept of proteomics and key techniques of proteomics.