Transfer of , , and corrig. into the novel genus gen. nov. and into the novel genus gen. nov. within the family .

During a phylogenetic analysis of and its closely related genera in the family , we found that the genus and the species might not be properly assigned in the taxonomy. Phylogenetic, phenotypic and chemotaxonomic characterizations clearly showed that the genus should be reclassified into two genera (Clade I and Clade II), for which the original genus name, , is proposed to be retained only for Clade I, and a new genus named as gen. nov.

sp. nov., isolated from tropical forest soil.

A Gram-stain-negative, aerobic and non-motile strain, designated 18x22-1, was isolated from a forest soil sample collected from Limushan Nature Reserve in Hainan Province, PR China. Growth occurred at 15-37 °C and pH 6.0-8.

Lysobacter silvisoli sp. nov., isolated from forest soil.

A yellow-pigmented, Gram-stain-negative, gliding and rod-shaped bacterial strain, designated zong2l5, was isolated from a forest soil sample at Dinghu Mountain, Guangdong Province, PR China. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain zong2l5 belongs to the genus Lysobacter, and was most closely related to Lysobacter enzymogenes KCTC 12131 (97.7 %) and Lysobacter soli KCTC 22011 (97.

Emended descriptions of the species Sphingomonas adhaesiva Yabuuchi et al. 1990 and Sphingomonas ginsenosidimutans Choi et al. 2011.

During the phylogenetic study of the genus Sphingomonas and its closely related genera, we found that there existed errors in the 16S rRNA gene sequence of the type strain of the type species of Sphingomonas adhaesiva (D13722). Data suggested the wrong sequence should be replaced by the sequence under the accession number KY927401. As the new sequence shared 99.

Reclassification of Sphingopyxis contaminans as Sphingorhabdus contaminans comb. nov. and emended description of the genus Sphingorhabdus.

With the description of the genus Sphingorhabdus, the taxonomic position of Sphingopyxis contaminans was re-evaluated based on analyses of 16S rRNA gene sequences and phenotypic and chemotaxonomic characteristics. The results revealed that Sphingopyxis contaminans is clearly a member of the genus Sphingorhabdus and we proposed that Sphingopyxis contaminans(Subhash Y, Sasikala C, Ramana CV. Int J Syst Evol Microbiol 2014;64:2238-2243) should be reclassified as Sphingorhabduscontaminans comb.

Sphingomonas spermidinifaciens sp. nov., a novel bacterium containing spermidine as the major polyamine, isolated from an abandoned lead-zinc mine and emended descriptions of the genus Sphingomonas and the species Sphingomonas yantingensis and Sphingomonas japonica.

A yellow-pigmented bacterial strain, designated 9NM-10T, was isolated from an abandoned lead-zinc mine in Meizhou, Guangdong Province, China. Cells were strictly aerobic, Gram-stain-negative and motile with a polar monotrichous flagellum. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 9NM-10T belongs to the genus Sphingomonas and was most closely related to Sphingomonas yantingensis JCM 19201T and Sphingomonas japonica JCM 15438T.

Corynebacterium guangdongense sp. nov., isolated from a contaminated plate.

A novel Gram-reaction-positive, non-motile and facultatively anaerobic bacterium, designated strain S01T, was isolated from a nutrient agar plate kept on a laboratory clean bench at Guangdong Institute of Microbiology, PR China, which was contaminated from an unknown source. Strain S01T was found to be catalase-positive and oxidase-negative. Similarity searches revealed that the strain shared the highest 16S rRNA gene similarity with Corynebacterium humireducens MFC-5T (95.

Sphingomonas metalli sp. nov., isolated from an abandoned lead-zinc mine.

A Gram-stain-negative and non-motile bacterial strain designated 9O-5T was isolated from an abandoned lead-zinc mine in Meizhou, Guangdong Province, southern China. The isolate was orange-pigmented, aerobic, and oxidase- and catalase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 9O-5T belongs to the genus Sphingomonas and was closely related to Sphingomonas abaci DSM 15867T (97.

Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing.

A heavy metal-resistant and dimethyl disulfide-producing bacterial strain, designated 6NM-7T, was isolated from wolfram mine tailing, Dayu County, Jiangxi Province, PR China. Strain 6NM-7T was aerobic, Gram-stain-negative and motile by means of a single polar flagellum. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain 6NM-7T was affiliated with the genus Massilia and was closely related to Massilia norwichensis LMG 28164T (98.

Lysobacter mobilis sp. nov., isolated from abandoned lead-zinc ore.

An aerobic and Gram-stain-negative bacterial strain, designated 9NM-14(T), was isolated from abandoned lead-zinc ore from Meizhou, Guangdong Province, south China. Strain 9NM-14(T) was motile by means of a single polar flagellum. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain 9NM-14(T) was affiliated with the genus Lysobacter and was most closely related to Lysobacter xinjiangensis RCML-52(T) and Lysobacter bugurensis ZLD-29(T) (97.

Acinetobacter guangdongensis sp. nov., isolated from abandoned lead-zinc ore.

A Gram-stain-negative, non-motile bacterial strain designated 1NM-4(T) was isolated from an abandoned lead-zinc ore mine site in Mei County, Meizhou, Guangdong Province, southern China. The isolate was light yellow, strictly aerobic, oxidase-negative and catalase-positive. Phylogenetic analyses based on 16S rRNA, rpoB and gyrB gene sequences, together with DNA-DNA hybridization values less than 70%, revealed that strain 1NM-4(T) belongs to the genus Acinetobacter and may represent a novel species.

Sphingomonas gimensis sp. nov., a novel Gram-negative bacterium isolated from abandoned lead-zinc ore mine.

A novel bacterial strain designated 9PNM-6(T) was isolated from an abandoned lead-zinc ore mine site in Meizhou, Guangdong Province, China. The isolate was found to be Gram-negative, rod-shaped, orange-pigmented, strictly aerobic, oxidase- and catalase-positive. Growth occurred at 0-4 % NaCl (w/v, optimum, 0 %), at pH 6.

Description of a Gram-negative bacterium, Sphingomonas guangdongensis sp. nov.

A Gram-stain-negative bacterial strain, designated 9NM-8T, was isolated from an abandoned lead-zinc ore in Mei county, Meizhou, Guangdong province, PR China. The isolate was orange-pigmented, aerobic, oxidase- and catalase-positive, motile with lophotrichous flagella and rod-shaped. Strain 9NM-8T grew optimally at pH 7.

Identification of volatile components in Chinese Sinkiang fermented camel milk using SAFE, SDE, and HS-SPME-GC/MS.

The volatile components of Chinese Sinkiang fermented camel milk were isolated by solvent assisted flavour evaporation (SAFE), simultaneous distillation extraction (SDE, dichloromethane and diethyl ether as solvent, respectively) and headspace solid-phase microextraction (HS-SPME, CAR/PDMS, PDMS/DVB and DVB/CAR/PDMS fibre extraction, respectively) and analysed by GC/MS. A total of 133 volatile components were identified under 6 different conditions, including 30 esters, 20 acids, 18 saturated alcohols, 15 unsaturated aliphatic alcohols, 8 saturated ketones, 9 saturated aldehydes, 8 unsaturated aliphatic aldehydes, 6 furans, 5 sulphur-containing compounds, 5 ethers, 5 lactones, 3 other compounds, and 1 unsaturated aliphatic ketone. Three pretreatment methods were compared, assisted by principal component analysis (PCA).

Identification of volatile components in yak butter using SAFE, SDE and HS-SPME-GC/MS.

The volatile components of yak butter were isolated by solvent-assisted flavour evaporation (SAFE), simultaneous distillation extraction (SDE; dichloromethane and diethyl ether as solvent, respectively) and headspace solid-phase microextraction (HS-SPME; CAR/PDMS, PDMS/DVB and DVB/CAR/PDMS fibre extraction, respectively) and were analysed by GC/MS. A total of 83 volatile components were identified under six different conditions, including 28 acids, 12 esters, 11 ketones, 10 lactones, 10 alcohols, 4 other compounds, 2 aldehydes, 2 unsaturated aldehydes, 1 furan, 1 sulphur-containing compound, 1 unsaturated alcohol and 1 unsatruated ketone. Among them, 51 were identified by SAFE, 58 by SDE (45 with dichloromethane as solvent and 41 with diethyl ether as solvent) and 40 by HS-SPME (26 with CAR/PDMS; 26 with PDMS/DVB and 32 with DVB/CAR/PDMS).

Streptomyces caeruleatus sp. nov., with dark blue diffusible pigment.

An actinomycete, designated strain GIMN4.002(T), was isolated from a tomato rhizosphere soil sample in Guangzhou, China. The strain produces white aerial mycelium and dark blue diffusible pigment on Gause's synthetic agar, and microscopic observation revealed that it produces looped chains of spiny spores.

Streptomyces lacticiproducens sp. nov., a lactic acid-producing streptomycete isolated from the rhizosphere of tomato plants.

A novel actinomycete, designated strain GIMN4.001(T), was isolated from the rhizosphere of tomato plants grown in Guangzhou, China. The strain produced greyish white aerial mycelia, lactic acid and a large quantity of double diamond-shaped crystals on potato dextrose agar and yeast extract-malt extract agar.

Microbacterium radiodurans sp. nov., a UV radiation-resistant bacterium isolated from soil.

Strain GIMN 1.002(T), a UV radiation-tolerant bacterium, was isolated from the upper sand layers of the Gobi desert, Xinjiang, China and characterized in order to determine its taxonomic position. Cells were Gram-reaction-positive, heterotrophic, strictly aerobic, short rods.

Streptomyces vietnamensis sp. nov., a streptomycete with violet blue diffusible pigment isolated from soil in Vietnam.

An actinomycete, designated strain GIMV4.0001(T), was isolated from a forest soil sample in Vietnam. It produced white aerial mycelium and violet-blue diffusible pigment on Gause's synthetic agar.