AI-driven mechanistic analysis of conformational dynamics in CNNM/CorC Mg transporters.

The CNNM/CorC Mg transporters are widely conserved in eukaryotes (cyclin M [CNNM]) and prokaryotes (CorC) and participate in various biological processes. Previous structural analyses of the CorC transmembrane domain in the Mg-bound inward-facing conformation revealed the conserved Mg recognition mechanism in the CNNM/CorC family; however, the conformational dynamics in the Mg transport cycle remain unclear because structures in other conformations are unknown. Here, we used AlphaFold structure prediction to predict the occluded-like and outward-facing-like conformations of the CorC and CNNM proteins and identified conserved hydrophilic interactions close to the cytoplasmic side in these conformations.

Mechano-Responsive Fluorescent Gel based on Tetraphenylethylene-Crosslinked Dynamic Covalent Network.

The rapid development of mechano-responsive fluorescence has been driven by its promising applications in the fields of sensors, information encryption, and anti-counterfeiting. However, designing mechanophores that can exhibit fluorescence changes under relatively low force remains challenging. In this study, a mechano-responsive fluorescent gel was developed using a tetraphenylethylene derivative as a cross-linker, producing a dynamic covalent network that exhibits increased fluorescence under tensile stress.

Clinical Significance of Combined Detection of CCL22 and IL-1 as Potential New Bronchial Inflammatory Mediators in Children's Asthma.

Backgrounds: Severe asthma is a significant health burden because children with severe asthma are vulnerable to medication-related side effects, life-threatening deterioration, and impaired quality of life. However, there is a lack of data to elucidate the role of inflammatory variables in asthma. This study aimed to compare the levels of inflammatory factors in serum and sputum in children with acute and stable asthma to those in healthy children and the ability to predict clinical response to azithromycin therapy.

Lysosome-Targeted Bifunctional Fluorescent Probe and Type I/II Photosensitizer for Viscosity Imaging and Cancer Photodynamic Therapy.

Abnormal lysosomal viscosity is closely associated with cancer progression, underscoring the need for bifunctional fluorescent probes and photosensitizers (PSs) that can both monitor viscosity and facilitate imaging-guided therapy for simultaneous cancer diagnosis and treatment. Despite advances in lysosome-targeted PSs development, few have demonstrated the ability to generate both Type I and Type II reactive oxygen species (ROS). In this study, we present BTTPA, a lysosome-targeted fluorescent probe and photosensitizer, designed to integrate cancer diagnosis via viscosity imaging and cancer treatment through photodynamic therapy (PDT).

[α-Cyperone Antagonizes Intestinal Mucosal Inflammatory Response Through Modulation of TLR4/NF-κB Signaling Pathway to Alleviate Crohn's Disease-Like Colitis in Mice].

Objective: To investigate the effect and potential mechanisms of α-cyperone (CYP) on Crohn's disease (CD) -like colitis induced by 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) in mice.

Methods: The mice were randomly and evenly divided into wild type (WT), TNBS, CYP and 5-aminosalicylic acid (5-ASA) groups, with 10 mice in each group. The symptoms of enteritis, the function and structure of the intestinal barrier, and the expression levels of inflammatory factors, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and gamma-interferon (IFN-γ), in the colon were assessed.

[Role of Abelson interactor 2 in progression and prognosis of gastric cancer and its regulatory mechanisms].

Objective: To explore the regulatory role of Abelson interactor 2 (ABI2) in progression and prognosis of gastric cancer.

Methods: TIMER2.0, GEPIA, Kaplan-Meier Plotter and DAVID databases were used to analyze ABI2 expression in pancancer and its association with the prognosis of gastric cancer.

Factors influencing the physical activity among pediatric cancer survivors based on the parent-based expansion of the Theory of Planned Behavior.

Purpose: This study descriptively analyzed the current status of physical activity among pediatric cancer survivors and their parents and further analyzed the factors influencing the physical activity among pediatric cancer survivors based on the parent-based expansion of the Theory of Planned Behavior.

Methods: A total of 271 pediatric cancer survivors and their parents were conveniently selected as participants for this cross-sectional survey conducted from June to November 2023. Using questionnaires to collect sociodemographic and physical activity data of pediatric cancer survivors and their parents, dimensions of the Theory of Planned Behavior among pediatric cancer survivors.

Analysis of Predictive Value of Cellular Inflammatory Factors and T Cell Subsets for Disease Recurrence and Prognosis in Patients with Acute Exacerbations of COPD.

Objective: To explore the predictive value of cellular inflammatory factors and T cell subsets for disease recurrence and prognosis in patients with acute exacerbations of chronic obstructive pulmonary disease (COPD).

Methods: Serum samples were collected from the two groups to detect and compare the levels of inflammatory cytokines [interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α)], T cell subsets (CD4+, CD8+), and clinical related indicators. Pearson correlation analysis was used to analyze the correlation between inflammatory cytokines, T cell subsets, and clinical indicators.

Characterization and phylogenetic analysis of the complete chloroplast genome sequence of (Rchb. f. 1854).

(Rchb. f.), an ornamental orchid known for vibrant flowers, has a 148,090 bp chloroplast genome with 36.

Production and characterization of novel/chimeric sophorose-rhamnose biosurfactants by introducing heterologous rhamnosyltransferase genes into Starmerella bombicola.

Glycolipid biosurfactant, sophorolipids (SLs) and rhamnolipids (RLs) can be widely used in agriculture, food and chemical industries. The different physicochemical properties of SLs and RLs, such as hydrophilic lipophilic value (HLB) and critical micelle concentration (CMC), determine they have different application focus. Researchers are still hoping to obtain new glycolipid surfactants with unique surface activities.

From inflammation to depression: key biomarkers for IBD-related major depressive disorder.

Background: Inflammatory bowel disease (IBD) is a chronic, inflammatory, and autoimmune disorder, and its incidence of comorbid with major depressive disorder (MDD) is significantly higher than the general population. However, many patients lack proper recognition and necessary psychological health treatments. We aimed to identify potential biomarkers and mechanisms involved in the development of IBD comorbid with MDD (IBD-MDD).

Target-centric analysis of hepatitis B: identifying key molecules and pathways for treatment.

Hepatitis B virus (HBV) poses a significant global health challenge, potentially leading to severe liver conditions, with currently limited effective treatment options available. Xiao-Chai-Hu-Tang (XCHT), a well-known Traditional Chinese Medicine (TCM) prescription, shows promise in clinical trials for treating HBV. Therefore, screening the complex components of XCHT, identifying the active compounds, and closely exploring the targets associated with hepatitis B may constitute an effective strategy for the development of new therapeutic drugs for the treatment of this disease.

Coexistence of DIPNECH with pulmonary adenocarcinoma: Coincidence or by design?

Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia (DIPNECH) coexisting with pulmonary adenocarcinoma (ADC) is rare. Herein, we report a new case and review the cases published previously in the English literature. The clinicopathological features of similar cases reported in the English literature were summarized, and a putative association between DIPNECH and pulmonary ADC was suggested.

Increased serum leptin levels are associated with metabolic syndrome and semen parameters in patients with infertility.

This study aims to ascertain the associations between serum leptin levels and metabolic syndrome and semen parameters in patients with infertility. A total of 200 patients who were diagnosed as primary infertility in our hospital were enrolled in this study, and they were divided into MetS group and non-MetS group. About 30 healthy men were enrolled as the control group.

Mediating role of circulating inflammatory proteins in the effect of immune cells on esophageal cancer risk: A Mendelian randomization study.

The immune system and inflammatory processes play crucial roles in the development of esophageal cancer (EC). This study aimed to investigate the causal relationships between 731 immune cell phenotypes, 91 circulating inflammatory proteins, and EC, with a particular focus on the mediating role of circulating inflammatory proteins. Utilizing public genetic data, we applied a 2-sample Mendelian Randomization (MR) method to examine the causal relationships between 731 immune cell phenotypes, 91 circulating inflammatory proteins, and EC.

Evidence for Nanostructures of at Least 20 nm in a Phosphonium Ionic Liquid at Room Temperature Using Fluorescence Correlation Spectroscopy.

Fluorescence correlation spectroscopy (FCS) measurements are performed on the ionic liquid (IL), tetradecyl(trihexyl) phosphonium chloride, [P66614][Cl], using fluorescent probes of varying sizes: ATTO 532, ∼2 nm; and 20- and 40 nm fluorescent beads. The fluorescence correlation function, (), is analyzed in terms of a distribution of diffusion coefficients using a maximum entropy method (MEM). For ATTO 532 and the 20 nm beads, the fit to () yields two well-defined distributions; for the 40 nm beads, however, only one is obtained.

Inhibition of Aβ Aggregation and Tau Phosphorylation with Functionalized Biomimetic Nanoparticles for Synergic Alzheimer's Disease Therapy.

The main pathological mechanisms of Alzheimer's Disease (AD) are extracellular senile plaques caused by β-amyloid (Aβ) deposition and intracellular neurofibrillary tangles derived from hyperphosphorylated Tau protein (p-Tau). However, it is difficult to obtain a good curative effect because of the poor brain bioavailability of drugs, which is attributed to the blood-brain barrier (BBB) restriction and complicated brain conditions. Herein, HM-DK was proposed for synergistic therapy of AD by using hollow mesoporous manganese dioxide (HM) as a carrier to deliver an Aβ-inhibiting peptide and a Dp-peptide inhibitor of Tau-related fibril formation synergistically.

A machine learning based radiomics approach for predicting No. 14v station lymph node metastasis in gastric cancer.

Purpose: To evaluate the potential of radiomics approach for predicting No. 14v station lymph node metastasis (14vM) in gastric cancer (GC).

Methods: The contrast enhanced CT (CECT) images with corresponding clinical information of 288 GC patients were retrospectively collected.

The HALP score predicts no-reflow phenomenon and long-term prognosis in patients with ST-segment elevation myocardial infarction after primary percutaneous coronary intervention.

Introduction And Objective: Despite recent advances in the management of ST-segment elevation myocardial infarction (STEMI), the clinical outcome of some patients is still unsatisfactory. Therefore, early evaluation to identify high-risk individuals in STEMI patients is essential. The hemoglobin, albumin, lymphocyte, and platelet (HALP) score, as a new indicator that can reflect both nutritional status and inflammatory state of the body, can provide prognostic information.

Organic-Inorganic Hybrid Perovskite for Ferroelectric Catalysis.

Organic-inorganic hybrid perovskite ferroelectric has gained significant attention for its structural flexibility and diversity. They can directly utilize metal nodes and organic groups as active sites in catalysis. Additionally, their ferroelectric polarization occurs around these active sites, significantly enhancing catalytic activity and demonstrating immense potential for applications.

Brief Report: Ivonescimab Combined With Etoposide Plus Carboplatin as First-Line Treatment for Extensive-Stage SCLC: Results of a Phase 1b Clinical Trial.

Introduction: Ivonescimab is a humanized IgG1 bispecific anti-programmed cell death protein 1/vascular endothelial growth factor antibody. This study aimed to evaluate the safety and tolerance of ivonescimab combined with etoposide and carboplatin as first-line treatment in patients with extensive-stage SCLC and to explore the primary efficacy of this regimen.

Methods: Eligible patients received intravenous ivonescimab 3 mg/kg, 10 mg/kg, or 20 mg/kg every 3 weeks combined with etoposide and carboplatin for up to four cycles, followed by ivonescimab as maintenance.