[Survey of infection characteristics and its risk factors among healthy blood donation population in Shijiazhuang City, Hebei Province].

Objective: To understand the prevalence of infection and its risk factors among healthy blood donation population in Shijiazhuang City, Hebei Province, so as to provide the evidence for developing effective prevention and control measures.

Methods: A total of 1 630 cases of blood donors in Shijiazhuang City were chosen as respondents, and all the respondents were qualified to the standard of blood donation. The antibodies to were detected by using ELISA.

[Awareness and infection of Toxoplasma gondii in married childbearing women in Chengde Region].

Objective: To understand Toxoplasma gondii infection and awareness condition of married childbearing women in Chengde Region, so as to provide the evidence for the establishment of control measures.

Methods: Totally 733 married childbearing women who took physical examination in Chengde Hospital of Traditional Chinese Medicine from July to December in 2013 were investigated by questionnaire to understand the awareness condition on T. gondii infection, then 490 women among them from 3 counties and 2 districts were randomly chosen to detect the Toxoplasma antibodies by ELISA.

[Toxoplasma gondii infection status in abnormal pregnancy women].

Objective: To investigate the relationship between Toxoplasma gondii (TOX) infection and abnormal pregnancy outcomes.

Methods: A total of 126 cases of abnormal pregnancy women in the Department of Obstetrics and Gynecology, the Fourth People' s Hospital of Langfang from March to December 2013 were chosen as an experimental group, and 263 cases of normal pregnancy women of childbearing age as a control group. The TOX-IgM and -IgG antibodies were detected by ELISA.

[Impact of Toxoplasma gondii infection on pregnancy outcomes in early pregnant women].

Objective: To explore the impact of Toxoplasma gondii infection on pregnancy outcomes in early pregnancy women.

Methods: Toxoplasma gondii IgM and IgG antibodies in the peripheral blood of 2 993 early pregnant women were detected by using enzyme-linked immunosorbent assay (ELISA). According to the test results, the infected ones were divided into an acute infection group, a previous infection group, and an active infection group, and 200 pregnant women without Toxoplasma infection were randomly chosen as a control group, and the pregnancy outcomes of the four groups were followed up and the results were compared.

[Investigation on pregnancy outcomes and risk factors in pregnant women infected with Toxoplasma gondii].

Objective: To understand the pregnancy outcomes and risk factors in pregnant women infected with Toxoplasma gondii.

Methods: Toxoplasma IgM and IgG antibodies in sera from 2 740 cases of pregnant women were detected by using enzyme -linked immunosorbent assay (ELISA) in Zhuozhou Municipal Maternal and Child Health Care Center from 2010 to 2013, and the pregnancy outcomes were followed up. The risk factors for Toxoplasma infection were investigated with questionnaires.

[Efficiency of three methods for detecting Toxoplasma IgG antibody].

Objective: To discuss the test efficiency of three methods for detecting Toxoplasma IgG antibody.

Methods: Totally 304 specimens were detected parallelly for Toxoplasma IgG antibody by using the gold marked method, indirect hemagglutination test (IHA), and enzyme-linked immunosorbent assay (ELISA), and the sensitivity, specificity and Youden index of these methods were compared.

Results: The detection sensitivities of gold marked method, IHA, and ELISA for Toxoplasma IgG antibody were 85.

[Prevalence of IgG antibodies specific to Toxoplasma gondii among college student blood donors in Shijiazhuang City].

Objective: To understand the epidemiological status of Toxoplasma gondii infection among blood donors in college students in Shijiazhuang City, China.

Methods: The blood samples were collected from the students in 4 universities and healthy adults in Shijiazhuang City, and the IgG antibodies specific to T. gondii were detected by using ELISA.

[Seroprevalence of Toxoplasma gondii antibodies in healthy voluntary blood donors from Shijiazhuang area].

The positive rate of Toxoplasma gondii antibodies in healthy voluntary blood donors in Shijiazhuang area was 4.83%, and it showed differences among the different regions, occupations, ages, education levels and nations. So the blood donors should receive the Toxoplasma infection screening in order to prevent toxoplasmosis from blood recipients.

[Effect of gold labeling immunoassay on field screening for Toxoplasma gondii infection of blood donors].

Objective: To evaluate the effect of gold labeling immunoassay (GLIA) on field screening for Toxoplasma gondii infection of blood donors.

Methods: A total of 1 056 specimens from the blood donors were examined by using GLIA and ELISA.

Results: The Toxoplasma IgG positive rates of GLIA and ELISA were 5.

[Relationship between toxoplasma IgG and routine screening items of infectious diseases of blood donors].

Among 745 blood donors whose routine screening items were unqualified, the positive rate of Toxoplasma IgG (TOX-IgG) was 21.9%, which was higher than that of the blood donors whose routine screening items were qualified, and there was a significant difference (P < 0.01).