Risk factor analysis and nomogram prediction model construction of postoperative complications of thoracoscopic non-small cell lung cancer.

Background: A series of complications will inevitably occur after thoracoscopic pulmonary resection. How to avoid or reduce postoperative complications is an important research area in the perioperative treatment of thoracic surgery. This study analyzed the risk factors for thoracoscopic postoperative complications of non-small cell lung cancer (NSCLC) and established a nomogram prediction model in order to provide help for clinical decision-making.

Case report of multiple primary cancers and results of genetic testing to preliminarily explore their pathogenesis.

The occurrence of multiple primary malignancies in a single patient has been relatively rare. We report here the case of a 71-year-old man with three primary tumors of lung cancer, intrahepatic cholangiocarcinoma, and prostate cancer, and a preliminary study of the mechanisms by which multiple primary tumors develop at the genetic level. Because of the late stage of the patient's condition, large tumor burden, and poor physical status, the patient survived only a few months.

Caveolin-1 Promotes the Imbalance of Th17/Treg in Chronic Obstructive Pulmonary Disease by Regulating Hsp70 Expression.

Objective: To investigate whether the expression of Hsp70 is associated with Cav-1 in promoting the imbalance of Th17/Treg cells in COPD.

Methods: The plasma Cav-1, Hsp70 expression were quantified by enzyme-linked immunosorbent assay (ELISA). The frequencies of circulating Th17, Treg cells and Th17/Treg ratio were determined by flow cytometry.

Identification of Immune-Related Prognostic Markers in Gastric Cancer.

Gastric cancer (GC) is a malignant tumor with a high fatality rate. Poor prognosis is the main cause of death caused by GC. In this study, the gene expression difference between GC and the control group was analyzed.

Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics.

Identification of the tyrosine phosphorylation (pY)-dependent interactome of immune co-receptors is crucial for understanding signal pathways involved in immunotherapy. However, identifying the motif-specific interactome for each pY commonly found on these multi-phosphorylated membrane proteins remains challenging. Here, we describe a photoaffinity-based chemical proteomic approach to dissect the motif-specific cytoplasmic interactomes of the critical immune co-receptor CD28.

CircFAM64A enhances cellular processes in triple-negative breast cancer by targeting the miR-149-5p/CDT1 axis.

Triple-negative breast cancer (TNBC) is a breast cancer subtype without targeted treatment options. Accumulating evidence has demonstrated the roles of circular RNAs in cancer. This study aimed to investigate the expression and function of circFAM64A in TNBC.

DSP-free remote antenna unit in a coherent radio over fiber mobile fronthaul for 5G mm-wave mobile communication.

We propose a novel coherent analog radio over fiber (A-RoF) scheme to realize the generation, separation, and detection of four-independent mm-wave signals with the same carrier frequency on a single-wavelength for 5 generation (5G) mobile communication, and no digital signal processing (DSP) algorithms are required in remote antenna unit (RAU). In baseband unit (BBU), four-independent mm-wave signals are modulated on the two orthogonal polarization states of a single wavelength based on a dual-polarization IQ modulator using the dual single-sideband (SSB) modulation and polarization division multiplexing (PDM) technique. In RAU, a novel carrier polarization rotation module based on the self-polarization stabilization technique is proposed, and thus the four-independent mm-wave signals can be detected by self-coherent detection.

Asymmetric dual-SSB modulation for photonic co-frequency mm-wave signals generation and DSP-free receiver.

An asymmetric dual-single-sideband (SSB) modulation scheme for photonic co-frequency millimeter (mm)-wave signals generation and digital signal processing (DSP)-free receiver is experimentally demonstrated for the first time, to the best of our knowledge. To effectively avoid the sideband crosstalk in the traditional symmetric dual-SSB modulation scheme, not only two vector-modulated signals but also two unmodulated sidebands are modulated on the two asymmetric sides of an optical carrier in this scheme. An optical delay line interferometer could easily separate these two asymmetric dual-SSB signals simultaneously in the receiver, and thus the photonic frequency up-conversion is realized.

Rectosigmoid-Junction Squamous Cell Carcinoma With pMMR/MSS Achieved a Partial Response Following PD-1 Blockade Combined With Chemotherapy: A Case Report.

Colorectal squamous cell carcinoma (SCC) is extremely rare and associated with a poor prognosis. And the pMMR/MSS colorectal cancer is related to a limited response to programmed death ligand-1 (PD-1) blockade monotherapy. However, the clinical activity of PD-1 blockade monotherapy or combination therapy in colorectal SCC is unknown.

Depleting long noncoding RNA HOTAIR attenuates chronic myelocytic leukemia progression by binding to DNA methyltransferase 1 and inhibiting PTEN gene promoter methylation.

Long noncoding RNAs (lncRNAs) are known to play a key role in chronic myelocytic leukemia (CML) development, and we aimed to identify the involvement of the lncRNA HOX antisense intergenic RNA (HOTAIR) in CML via binding to DNA methyltransferase 1 (DNMT1) to accelerate methylation of the phosphatase and tensin homolog (PTEN) gene promoter. Bone marrow samples from CML patients and normal bone marrow samples from healthy controls were collected. HOTAIR, DNMT1, DNMT3A, DNMT3B, and PTEN expression was detected.

Neoantigen-reactive T cells exhibit effective anti-tumor activity against colorectal cancer.

Neoantigens play a crucial role in cancer immunotherapy. However, the effectiveness and safety of neoantigen-based immunotherapies in patients with colorectal cancer (CRC), particularly in the Chinese population, have not been well studied. This study explored the feasibility and effectiveness of neoantigens in the treatment of CRC.

Successful recovery of a patient with multiple myeloma from severe coronavirus disease 2019 (COVID-19) pneumonia during the first chemotherapy cycle: A case report.

A continuing outbreak of pneumonia associated with the 2019 novel coronavirus (2019-nCoV) was initially described in Wuhan, China in December 2019. Weak and elderly individuals, and those with chronic diseases such as hematological malignancies are prone to develop severe pneumonia. The humoral immunity of patients with multiple myeloma is prevalently low, and their inferior immunity further deteriorates during chemotherapy.

Long Noncoding RNAs: New Regulators of Resistance to Systemic Therapies for Gastric Cancer.

Gastric cancer (GC) is the second leading cause of cancer mortality and the fourth most commonly diagnosed malignant disease, with approximately 951,000 new cases diagnosed and approximately 723,000 cases of mortality each year. The highest mortality rate of GC is in East Asia, and the lowest is in North America. A large number of studies have demonstrated that GC patients are characterized by higher morbidity, metastasis rates, and mortality and lower early diagnosis rates, radical resection rates, and 5-year survival rates.

Low-complexity equalization scheme for suppressing FFE-enhanced in-band noise and ISI in 100 Gbps PAM4 optical IMDD system.

The commonly used feed-forward equalizer (FFE) in an intensity modulation and direct-detection (IMDD) system is seriously restrained by the enhanced in-band noise in the high-frequency region. In this Letter, a low-complexity equalization scheme including FFE, post-filter (PF), and a newly designed interference cancellation (IC) algorithm is proposed to cope with the FFE-enhanced noise and serious inter-symbol interference (ISI) simultaneously. In our experiment, the achieved bit error rate value of 100 Gb/s optical double-sideband (ODSB) four-level pulse amplitude modulation (PAM4) signal transmission over 10 km standard single-mode fiber (SSMF) can go below the 20% overhead soft-decision forward error correction threshold of ${2.

SFPQ Is an FTO-Binding Protein that Facilitates the Demethylation Substrate Preference.

The fat mass and obesity-associated protein (FTO) is the first identified demethylase of the internal RNA modification N-methyladenosine (mA), which also exhibits demethylation activity toward N,2'-O-dimethyladenosine (mA) and N-methyladenosine (mA). Demethylation of mA at specific sites on target transcripts is a key enzymatic function of FTO that modulates diverse physiological and/or pathological processes. However, how FTO selects target RNA and whether additional interaction proteins facilitate this process remain elusive.

LncRNA, a novel target biomolecule, is involved in the progression of colorectal cancer.

Colorectal cancer is one of the most commonly diagnosed malignancies among males and females worldwide. Although China is a country with a low incidence of colorectal cancer, with the improvement of China's economy and lifestyle changes, the incidence rate in China has generally increased in recent years, and the morbidity and mortality of colorectal cancer rank fifth among those of all malignant tumours. Furthermore, despite recent improvements in screening strategies and treatments for colorectal cancer, the prognosis of advanced colorectal cancer is still poor, mainly due to the recurrence or distant metastasis of this disease.

Columnar cell papillary thyroid carcinoma prognosis: findings from the SEER database using propensity score matching analysis.

Background: Columnar cell papillary thyroid carcinoma (CCPTC) is a rare variant of papillary thyroid carcinoma (PTC), whose prognosis, as defined by the American Thyroid Association (ATA) guidelines, is considered poor, although available evidence is insufficient for reliable assessment. This study aimed to investigate the CCPTC prognosis using the Surveillance, Epidemiology, and End Results (SEER) database.

Methods: Data of thyroid cancer patients, recorded from 2004 to 2013, were extracted to assess the CCPTC prognosis.

Amplifier-free 4×96 Gb/s PAM8 transmission enabled by modified Volterra equalizer for short-reach applications using directly modulated lasers.

By using four O-band directly modulated lasers (DMLs), for the first time a 384 Gb/s (4 × 96 Gbit/s) 8-level pulse amplitude modulation (PAM8) signal is successfully transmitted over a 15 km standard single mode fiber (SSMF) with no optical amplifier. The nonlinear Volterra equalizer is usually used to cope with the distortions induced by the nonlinearity of DML and the bandwidth-limited components. However, the Volterra equalizer would also enhance the noise at high frequency, which is harmful, especially to PAM8 signal because it is more sensitive to noise.

Androgen deprivation promotes neuroendocrine differentiation and angiogenesis through CREB-EZH2-TSP1 pathway in prostate cancers.

The incidence of aggressive neuroendocrine prostate cancers (NEPC) related to androgen-deprivation therapy (ADT) is rising. NEPC is still poorly understood, such as its neuroendocrine differentiation (NED) and angiogenic phenotypes. Here we reveal that NED and angiogenesis are molecularly connected through EZH2 (enhancer of zeste homolog 2).

A Reliable and Real-Time Tracking Method with Color Distribution.

Occlusion is a challenging problem in visual tracking. Therefore, in recent years, many trackers have been explored to solve this problem, but most of them cannot track the target in real time because of the heavy computational cost. A spatio-temporal context (STC) tracker was proposed to accelerate the task by calculating context information in the Fourier domain, alleviating the performance in handling occlusion.

Increased cycles of DC/CIK immunotherapy decreases frequency of Tregs in patients with resected NSCLC.

Regulatory T cells (Tregs) suppress antitumor immune responses. Cycles of Dendritic cells (DC) vaccination combined with cytokine-induced killer (CIK) cells (DC/CIK) treatment were significantly related with good prognosis. Therefore, we investigated whether increased cycles of immunotherapy could decrease frequency of Tregs in patients with resected non-small cell lung cancer (NSCLC).

Quantitative and Comparative Profiling of Protease Substrates through a Genetically Encoded Multifunctional Photocrosslinker.

A genetically encoded, multifunctional photocrosslinker was developed for quantitative and comparative proteomics. By bearing a bioorthogonal handle and a releasable linker in addition to its photoaffinity warhead, this probe enables the enrichment of transient and low-abundance prey proteins after intracellular photocrosslinking and prey-bait separation, which can be subject to stable isotope dimethyl labeling and mass spectrometry analysis. This quantitative strategy (termed isoCAPP) allowed a comparative proteomic approach to be adopted to identify the proteolytic substrates of an E.

Genetically encoded releasable photo-cross-linking strategies for studying protein-protein interactions in living cells.

Although protein-protein interactions (PPIs) have crucial roles in virtually all cellular processes, the identification of more transient interactions in their biological context remains challenging. Conventional photo-cross-linking strategies can be used to identify transient interactions, but these approaches often suffer from high background due to the cross-linked bait proteins. To solve the problem, we have developed membrane-permeable releasable photo-cross-linkers that allow for prey-bait separation after protein complex isolation and can be installed in proteins of interest (POIs) as unnatural amino acids.