Evaluation of hydrogen storage in sandstone reservoirs using H nuclear magnetic resonance spectroscopy.

Evaluation of the hydrogen storage capacity of porous rocks is crucial for underground hydrogen storage. Using H nuclear magnetic resonance (NMR) spectroscopy, we successfully characterized the hydrogen responses and identified storage mechanisms in Berea sandstone under varying water saturation. The results indicate that the injected hydrogen behaves as a free gas phase and is capable of occupying the empty pore volume regardless of the saturation state.

Zona pellucida removal resulted in a successful live birth: Report on a case with recurrent implantation failure due to embryonic bacteria contamination.

Key Clinical Message: In in vitro fertilization (IVF), laser offers several advantages. In this study, we employed laser to eliminate the zona pellucida of a contaminated embryo. This approach helps to rescue embryo with bacterial contamination, and improve embryo-endometrium interaction.

PDGF receptor signal mediates the contribution of Nestin-positive cell lineage to subcutaneous fat development.

The beiging of white adipose tissue (WAT) is expected to improve systemic metabolic conditions; however, the regulation and developmental origin of this process remain insufficiently understood. In the present study, the implication of platelet-derived growth factor receptor alpha (PDGFRα) was examined in the beiging of inguinal WAT (ingWAT) of neonatal mice. Using in vivo Nestin expressing cell (Nestin) lineage tracing and deletion mouse models, we found that, in the mice with Pdgfra gene inactivation in Nestin lineage (N-PRα-KO mice), the growth of inguinal WAT (ingWAT) was suppressed during neonatal periods as compared with control wild-type mice.

Vascular PDGFR-alpha protects against BBB dysfunction after stroke in mice.

Blood-brain barrier (BBB) dysfunction underlies the pathogenesis of many neurological diseases. Platelet-derived growth factor receptor-alpha (PDGFRα) induces hemorrhagic transformation (HT) downstream of tissue plasminogen activator in thrombolytic therapy of acute stroke. Thus, PDGFs are attractive therapeutic targets for BBB dysfunction.

Escherichia coli contamination of fish raised in integrated pig-fish aquaculture systems in Vietnam.

Integrated livestock-fish aquaculture utilizes animal excreta and urine as pond fertilizers to enhance growth of plankton and other microorganisms eaten by the fish. In Vietnam, pigs are commonly integrated with fish and horticulture in household-based VAC systems (vuon = garden; ao = pond; chuong = pigsty), but the level of fecal contamination in the fish produced is unknown. This study was carried out to assess the level Escherichia coli contamination of fish meat and gut contents of grass carp (Ctenopharyngodon idellus), silver carp (Hypophthalmichthys molitrix), and rohu (Labeo rohita) cultured in randomly selected five VAC ponds (with pig manure) and five non-VAC ponds (without pig manure) at sites in periurban Hanoi, Vietnam.

Survival of faecal indicator bacteria in treated pig manure stored in clay-covered heaps in Vietnam.

Small-scale pig farmers in Vietnam typically store manure in heaps covered by a layer of clay. However, the hygienic quality of manure applied to agricultural soil is uncertain as storage time is determined by the need for soil fertilizer. This study aimed to assess how the storage of pig manure added straw, lime stone or urea in heaps covered by clay affected the survival of faecal bacteria.

Impact of medicated feed on the development of antimicrobial resistance in bacteria at integrated pig-fish farms in Vietnam.

Integrated livestock-fish aquaculture utilizes animal excreta, urine, and feed leftovers as pond fertilizers to enhance the growth of plankton and other microorganisms eaten by the fish. However, antimicrobial-resistant bacteria may be transferred and develop in the pond due to selective pressure from antimicrobials present in animal feed, urine, and feces. In an experimental pig-fish farm located in periurban Hanoi, Vietnam, nine piglets were provided feed containing 5 μg of tetracycline (TET)/kg pig weight/day and 0.