Identification and characterization of CD83 and CD276 as markers of dendritic cells in olive flounder (Paralichthys olivaceus).

Dendritic cells (DCs) play a pivotal role in activating naïve T-cells and bridging innate and adaptive immunity. This study aimed to identify and characterize CD83 and CD276 as potential markers for DCs in olive flounder (Paralichthys olivaceus). Specific antibodies against these markers were developed and used to analyze their distribution in peripheral blood leukocytes (PBLs) and intestinal tissues.

Ultrasound-assisted dieckol extraction from Eisenia bicyclis and its evaluation as a functional ingredient for bacterial cellulose-based desserts.

Dieckol is a brown algal phlorotannin with potent bioactivities such as hepatoprotective effects. This study aimed to produce dieckol-rich extract from Eisenia bicyclis and apply them as a functional ingredient for a novel bacterial cellulose (BC)-based dessert. The modeling and optimization of the ultrasound-assisted extraction (UAE) process were performed.

Multi-omic insights into molecular mechanism and therapeutic targets in spinocerebellar ataxia type 7.

Recent advances in molecular science have significantly enlightened our mechanistic understanding of spinocerebellar ataxia type 7. To further close remaining gaps, we performed a multi-omics analysis using SCA7 mice. Entire brain tissue samples were collected from 12-week-old mice, and RNA sequencing, methylation analysis, and proteomic analysis were performed.

A Facile Approach To Develop Ion Pair Micelles Satellited Freshly Derived Neutrophils For Targeted Tumor Therapy.

Immune cells show enormous potential for targeted nanoparticle delivery due to their intrinsic tumor-homing skills. However, the immune cells can internalize the nanoparticles, leading to cellular functional impairments, degradation of the nanoparticles, and delayed release of drugs from the immune cells. To address these issues, this study introduces an approach for the synthesis of freshly derived neutrophils (NUs)-based nanocarriers system where the NUs are surfaced by dialdehyde alginate-coated self-assembled micelles loaded with mitoxantrone (MIT) and indocyanine green (ICG) (i.

Clinical spectrum of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia in individuals of Korean ancestry.

Adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) is a rare white matter disease characterized by axonal and glial injury. Although its clinical characteristics have been described in case reports, the prevalence of CSF1R mutations in clinically suspected ALSP cases remains unclear. Herein, we analysed the frequency of CSF1R mutations in patients with probable or possible ALSP and describe the genetic, clinical, radiological, and pathological findings of ALSP cases in individuals of Korean ancestry.

Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva () and Silkworm Pupa ().

The increasing global population and the environmental consequences of meat consumption have led to the exploration of alternative sources of protein. Edible insects have gained attention as a sustainable and nutritionally rich meat alternative. We investigated the effects of two commonly consumed insects, larva and pupa, on beneficial gut microbiota growth, using whole 16s metagenome sequencing to assess diet-associated changes.

sp. nov., isolated from human epidermis.

Five pink-pigmented bacterial strains, isolated from human skin and classified within the genus , were examined. Among them, four were identified as , while strain OT10 was deemed to be a potential novel species. Strain OT10 exhibited characteristics, such as Gram-stain-negative, oxidase positive, motile, strictly aerobic and rod shaped.

CD4+ T helper 2 cell-macrophage crosstalk induces IL-24-mediated breast cancer suppression.

CD4+ T cells contribute to antitumor immunity and are implicated in the efficacy of cancer immunotherapies. In particular, CD4+ T helper 2 (Th2) cells were recently found to block spontaneous breast carcinogenesis. However, the antitumor potential of Th2 cells in targeting established breast cancer remains uncertain.

Management Strategies for Potassium Levels During Non-steroidal Mineralocorticoid Receptor Antagonist Therapy: A Comprehensive Review.

Diabetic kidney disease (DKD) is a leading cause of chronic kidney disease (CKD). Recent advancements highlight the role of finerenone, a non-steroidal mineralocorticoid receptor antagonist (nsMRA), in DKD management. Studies like FIDELIO-DKD, FIGARO-DKD, and FIDELITY have demonstrated finerenone's efficacy in reducing CKD progression and cardiovascular risks in DKD patients.

Population-level amplification of gene regulation by programmable gene transfer.

Engineering cells to sense and respond to environmental cues often focuses on maximizing gene regulation at the single-cell level. Inspired by population-level control mechanisms like the immune response, we demonstrate dynamic control and amplification of gene regulation in bacterial populations using programmable plasmid-mediated gene transfer. By regulating plasmid loss rate, transfer rate and fitness effects via Cas9 endonuclease, F conjugation machinery and antibiotic selection, we modulate the fraction of plasmid-carrying cells, serving as an amplification factor for single-cell-level regulation.

Real-time continuous glucose monitoring improves postoperative glucose control in people with type 2 diabetes mellitus undergoing coronary artery bypass grafting: A randomized clinical trial.

Background: Effective glycaemic control following cardiac surgery improves clinical outcomes, and continuous glucose monitoring (CGM) might be a valuable tool in achieving this objective. We investigated the effect of real-time CGM and telemonitoring on postoperative glycaemic control in people with type 2 diabetes (T2D) after coronary artery bypass grafting (CABG).

Methods: In this randomized clinical trial (RCT), adults with T2D undergoing CABG were assigned to either a test group utilizing real-time CGM (Dexcom G6) and telemetry for glycaemic control, or a control group with blinded CGM measures, relying on point-of-care measures.

Endocrine Mucin-Producing Sweat Gland Carcinoma (EMPSGC) in a Dog: Immunohistochemical Characterization.

A seven-year-old spayed female dog presented with multiple, small oval-shaped masses on the abdominal skin. The excised tissue was submitted for histopathological evaluation. Routine histology revealed basaloid nodules with stippled chromatin and pleomorphic nuclei.

The Factors Affecting Sleep Quality in Community-Dwelling Older Adults, Based on Spielman's 3P Model of Insomnia.

Background: We aimed to identify the factors affecting sleep quality in community-dwelling older adults.

Methods: This cross-sectional descriptive study was conducted from 6 to 13 October 2021. A hypothetical model based on Spielman's 3P model of insomnia was constructed using the multiple factors that influence sleep quality in older adults.

T helper 2 cell-directed immunotherapy eliminates precancerous skin lesions.

The continuous rise in skin cancer incidence highlights an imperative for improved skin cancer prevention. Topical calcipotriol-plus-5-fluorouracil (calcipotriol-plus-5-FU) immunotherapy effectively eliminates precancerous skin lesions and prevents squamous cell carcinoma (SCC) in patients. However, its mechanism of action remains unclear.

The Influence of Dietary Habits on Male Lower Urinary Tract Symptoms: Differential Influence of Water Intake on Voiding Symptoms and Storage Symptoms.

Purpose: To investigate the dietary factors affecting male lower urinary tract symptoms (LUTS).

Materials And Methods: This retrospective study analyzed men who underwent health check-ups. The men who completed the International Prostate Symptom Score (IPSS) and a dietary questionnaire with 19 items were included in the study.

Mechanical Deformation Behavior of Polymer Blend Thin Films.

Examining the mechanical properties of polymer thin films is crucial for high-performance applications such as displays, coatings, sensors, and thermal management. It is important to design thin film microstructures that excel in high-demand situations without compromising mechanical integrity. Here, a polymer blend of polystyrene (PS) and polyisoprene (PI) is used as a model to explore microscale deformation behavior under uniaxial mechanical testing.

CD8+ cell dominance in immune checkpoint inhibitor-induced colitis and its heterogeneity across endoscopic features.

Background: Immune checkpoint inhibitor (ICI)-induced colitis is a significant adverse event associated with ICI therapy, known to be linked to increased cytotoxic T-cell activity.

Objectives: To compare T-cell subsets based on the endoscopic features of ICI-induced colitis and to compare these findings with those of inflammatory bowel disease (IBD).

Design: Prospective cohort study.

Lateralizing Value of Artificial Intelligence-Based Segmentation Software in MRI-Negative Focal Epilepsy.

Background And Purpose: The magnetic resonance images (MRIs) ability of lesion detection in epilepsy is crucial for a diagnosis and surgical outcome. Using automated artificial intelligence (AI)-based tools for measuring cortical thickness and brain volume originally developed for dementia, we aimed to identify whether it could lateralize epilepsy with normal MRIs.

Methods: Non-lesional 3-Tesla MRIs of 428 patients diagnosed with focal epilepsy, based on semiology and electroencephalography findings, were analyzed.

Real-Time Monitoring of Volatile Organic Compound-Mediated Plant Intercommunication Using Surface-Enhanced Raman Scattering Nanosensor.

Plants communicate through volatile organic compounds (VOCs), but real-time monitoring of VOCs for plant intercommunication is not practically possible yet. A nanobionic VOC sensor plant is created to study VOC-mediated plant intercommunication by incorporating surface-enhanced Raman scattering (SERS) nanosensors into a living plant. This sensor allows real-time monitoring of VOC with a sensitivity down to the parts per trillion level.

Posttraumatic hyperalgesia and associated peripheral sensitization after temporomandibular joint injury in mice.

Temporomandibular disorder (TMD) is the most prevalent painful condition in the craniofacial area. Recent studies have suggested that external or intrinsic trauma to the temporomandibular joint (TMJ) is associated with the onset of painful TMD in patients. Here, we investigated the effects of TMJ trauma through forced-mouth opening (FMO) in mice to determine pain behaviors and peripheral sensitization of trigeminal nociceptors in both sexes.

The Obesity Paradox Revisited: Is Obesity Still a Protective Factor for Patients With High Comorbidity Burden or High-Complexity Procedures?

Objective: To investigate the relationship between obesity and postoperative mortality in the context of high procedural complexity and comorbidity burden.

Background: The "obesity paradox" suggests better postoperative outcomes in patients with higher body mass index (BMI), despite obesity's associated health risks. Research remains scarce on the influence of procedural complexity and comorbidities on the obesity-postoperative mortality relationship.

Cutaneous Immune Responses to Ablative Fractional Laser, Heat- and Cold-Based Dermatological Procedures.

Objective: Physical treatment modalities, such as ablative fractional laser (AFL), electrocautery, and cryotherapy, are extensively used in the field of dermatology. This study aimed to characterize the short-term innate and adaptive immune responses induced by AFL compared with heat- and cold-based procedures.

Materials And Methods: Innate (CD11bLy6G neutrophils) and adaptive (CD8CD3 T cells) immune cell infiltration and histopathological changes were examined in murine skin on Days 1 and 7, following AFL, monopolar-electrocautery (RF), thermocautery, and cryotherapy.

Surgeon Adoption of Immediate Sequential Bilateral Cataract Surgery in the United States from 2018 to 2022: Immediate Sequential Bilateral Cataract Surgery.

Purpose: To assess 5-years trends in the rate of immediate sequential bilateral cataract surgery (ISBCS) and surgeon characteristics associated with performing ISBCS.

Setting: 100% Medicare Fee-for-service beneficiaries from 2018-2022.

Design: Cross-sectional study.