Publications by authors named "Somchai Thiemmeca"
Article Synopsis
- Dengue virus (DENV) infection is a major global health concern, and a new whole-blood model using hirudin has been developed to study its immunopathogenesis more effectively.
- This model shows that all major leukocyte populations, especially monocytes and granulocytes, are highly susceptible to DENV, with granulocytes identified as new key targets for the virus.
- The study also confirms that B, NK, and T cells can be infected, indicating complex interactions within the immune response, and highlights the potential for this model to improve understanding of dengue disease and aid in developing new treatments.
Dengue virus (DENV) infection is a significant global health problem. There are no specific therapeutics or widely available vaccines. Early diagnosis is critical for patient management.
View Article and Find Full Text PDFDetection and quantification of viruses in laboratory and clinical samples are standard assays in dengue virus (DENV) studies. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) is considered to be the standard for DENV detection and quantification due to its high sensitivity. However, qRT-PCR offers only quantification relative to a standard curve and consists of several "in-house" components resulting in interlaboratory variations.
View Article and Find Full Text PDFArticle Synopsis
- Developing suitable cell models is crucial for understanding liver diseases and creating effective treatments.
- Researchers created an immortalized hepatocyte-like cell line (imHC) from human mesenchymal stem cells to study dengue virus (DENV) as a potential alternative to expensive and variable primary human hepatocytes (PHHs).
- Findings indicated that imHC cells show better DENV replication, unique cellular responses, and greater efficacy for anti-viral drug testing compared to traditional hepatoma cell lines, making them a valuable model for liver disease research.
Flavivirus nonstructural protein 1 (NS1) is a unique secreted nonstructural glycoprotein. Although it is absent from the flavivirus virion, intracellular and extracellular forms of NS1 have essential roles in viral replication and the pathogenesis of infection. The fate of NS1 in insect cells has been more controversial, with some reports suggesting it is exclusively cell associated.
View Article and Find Full Text PDFUnlabelled: Shedding of microparticles (MPs) is a consequence of apoptotic cell death and cellular activation. Low levels of circulating MPs in blood help maintain homeostasis, whereas increased MP generation is linked to many pathological conditions. Herein, we investigated the role of MPs in dengue virus (DENV) infection.
View Article and Find Full Text PDFVascular permeability, thrombocytopenia, liver pathology, complement activation, and altered hemostasis accompanying a febrile disease are the hallmarks of the dengue hemorrhagic fever/dengue shock syndrome, a major arthropod-borne viral disease that causes significant morbidity and mortality throughout tropical countries. We studied tissues from 13 children who died of acute dengue hemorrhagic fever/dengue shock syndrome at the Childrens' Hospital, Yangon, Myanmar. Dengue viral RNA from each of the 4 dengue viruses (DENVs) was detected by reverse transcriptase polymerase chain reaction in 11 cases, and dengue viral proteins (envelope, NS1, or NS3) were detected in 1 or more tissues from all 13 cases.
View Article and Find Full Text PDFDengue virus infection is an important mosquito-borne disease and a public health problem worldwide. A better understanding of interactions between human cellular host and dengue virus proteins will provide insight into dengue virus replication and cellular pathogenesis. The glycosylated envelope protein of dengue virus, DENV E, is processed in the endoplasmic reticulum of host cells and therefore reliant on host processing functions.
View Article and Find Full Text PDFSensitization to Fas-mediated apoptosis by dengue virus capsid protein.
Biochem Biophys Res Commun
October 2007
Dengue fever (DF) and dengue hemorrhagic fever (DHF) are important public health problems in tropical regions. Abnormal hemostasis and plasma leakage are the main patho-physiological changes in DHF. However, hepatomegaly, hepatocellular necrosis and fulminant hepatic failure are occasionally observed in patients with DHF.
View Article and Find Full Text PDFBackground: Vascular leakage and shock are the major causes of death in patients with dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Thirty years ago, complement activation was proposed to be a key underlying event, but the cause of complement activation has remained unknown.
Methods: The major nonstructural dengue virus (DV) protein NS1 was tested for its capacity to activate human complement in its membrane-associated and soluble forms.