HOXA10 protein expression in the endometrium of normally menstruating women after receiving GnRH antagonist.

Objective(s): To compare HOXA10 protein expression in the endometrium between natural control cycles and GnRH antagonist-treated cycles obtained during the window of implantation of normally menstruating women.

Study Design: This study was conducted at the Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Thirty-five volunteers were recruited into this prospective, self-controlled study, which was divided into two cycles, the first a natural control cycle and the second a GnRH antagonist-treated cycle.

Current opinion on use of luteinizing hormone supplementation in assisted reproduction therapy: an Asian perspective.

LH and FSH have complementary functions in ensuring optimal oocyte maturation and ovulation. In women undergoing assisted reproduction technology protocols with gonadotrophin-releasing hormone analogues, LH and FSH concentrations are reduced. While FSH use in assisted reproduction technology is well established, there is no published consensus on the need for exogenous LH in Asian patients.

The effects of GnRH antagonist on the endometrium of normally menstruating women.

Purpose: To study the effects of GnRH antagonist (ganirelix-Orgalutran) on the endometrium of regularly menstruating women.

Materials And Methods: Prospective, self-controlled study. The thirty-five volunteers were studied for two cycles: one as a control and the other, GnRH antagonist-treated cycles in which ganirelix 0.

The salivary ferning test and ovulation in clomiphene citrate-stimulated cycles.

Objective: To determine the day of ovulation by the salivary ferning test in clomiphene citrate-treated women.

Design: A descriptive study.

Setting: Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Results of direct current electrical activation of failed-to-fertilize oocytes after intracytoplasmic sperm injection.

Objective: To assess the embryo formation rate after electrical activation on failed-to-fertilize oocytes after intracytoplasmic sperm injection (ICSI).

Study Design: A prospective, randomized, experimental study was conducted in our research laboratory at the Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, between January and September 2004. One hundred failed-to-fertilize oocytes after ICSI were randomly assigned by stratified allocation according to oocyte grading before ICSI.

Comparison of two different fixed doses of follitropin-beta in controlled ovarian hyperstimulation: A prospective randomized, double blind clinical trial.

A prospective randomized, double blind, single centre study was conducted to compare the efficacy, efficiency and clinical side effects of daily fixed dose regimen of either 100 IU or 200 IU of recombinant follicle stimulating hormone(rFSH) Follitropin beta in down-regulated women undergoing controlled ovarian hyperstimulation(COH) for either conventional in vitro fertilization(IVF) or intracytoplasmic sperm injection(ICSI). A total of sixty women were randomly allocated according to the criteria for the treatment by either 100 IU(n = 30) or 200 IU (n = 30) of FSH. Although more cycle cancellations due to low response were observed in the 100 IU group (n = 9 vs n = 2), two cases of mild and moderate ovarian hyperstimulation syndrome were noted in the higher dose group.

The preemptive analgesic effect of celecoxib for day-case diagnostic laparoscopy.

In a randomized trial, the preemptive analgesic effect of celecoxib in 110 infertile women undergoing day-case diagnostic laparoscopy was studied at King Chulalongkorn Memorial Hospital, Bangkok, Thailand. The patients randomly received either 200 mg celecoxib or placebo orally 2 hours before diagnostic laparoscopy. The post-operative shoulder pain and wound pain were self assessed and recorded, using Visual Analogue Scores (VAS) at 1, 2, 4, 12, and 24 hours.

Laparoscopic finding in Thai women with chronic pelvic pain.

Objective: To study the laparoscopic findings in Thai women with chronic pelvic pain.

Setting: The Gynecology Endoscopy unit, Department of Obstetrics and Gynecology, Faculty of Medicine, King Chulalongkorn Memorial hospital.

Design: Descriptive study.

Correlation between human follicular diameter and oocyte outcomes in an ICSI program.

Purpose: To determine the correlation between the follicular sizes and oocyte recovery, metaphase II oocyte recovery, fertilization rate and good embryo quality from mature and immature oocytes in an intracytoplasmic sperm injection (ICSI) program.

Methods: 991 follicles obtained from 72 ICSI cycles were classified into three groups according to their diameters as measured by transvaginal ultrasound including group A (< 10 mm), group B (10-14 mm), and group C (> 14 mm). All obtained oocytes were classified according to their nuclear maturation: germinal vesicle (GV), metaphase I (MI) and metaphase II (MII).

Preliminary study on somatic cell nuclear transfer in rabbits in Thailand.

The objective of the study was to develop the somatic nuclear transfer technique by using rabbits as the model. The oocyte recipients aged 16 h post coitus were collected surgically from 20 superovulated rabbit doe with 28 and 40 mg Follicle Stimulating Hormone (FSH) after mating with a vasectomized male. The metaphase II plate and 1st polar body of oocyte was later aspirated by enucleated micropipette under an inverted microscope.

The effect of growth hormone on the development of in vitro matured unstimulated human oocytes.

Objective: To investigate the effect of growth hormone on the development of in vitro matured unstimulated human oocytes.

Design: Randomized controlled study.

Setting: Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn university.

Predictive value of human chorionic gonadotropin in the outcome of early pregnancy achieved by assisted reproductive technology.

This study was undertaken to evaluate the predictive value of the serum human chorionic gonadotropin (hCG) in pregnancies achieved by assisted reproductive techonology (ART). Two hundred and eighty-six pregnancies were studied retrospectively from September 1989 to June 1998. The serum hCG samples at 2-6 weeks after embryo transfer (ET) were analysed by fluoroimmunoassay.

Serum beta-hCG regression after laparoscopic salpingostomy in the treatment of ectopic pregnancy in Thai women.

This is a descriptive study which reviewed the regression pattern of serum beta hCG in Thai women with ectopic pregnancy after laparoscopic salpingostomy. The study group included the medical records of 21 patients who underwent laparoscopic salpingostomy between January 1991 and August 2001. The results revealed that the regression of serum beta hCG in patients with ectopic pregnancy after laparoscopic salpingostomy was about 10, 100, 1,000 times at day 6, 20 and 35 after operation.

Effects of initiation day of clomiphene citrate on the endometrium of women with regular menstrual cycles.

Objective: To investigate the effects of initiation time of clomiphene citrate (CC) on the endometrium of women with regular menstrual cycles.

Design: Randomized, double-blind, cross-over study.

Setting: Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.