Effects of Boron Neutron Capture Therapy on the Growth of Subcutaneous Xenografts of Human Colorectal Adenocarcinoma SW-620 in Immunodeficient Mice.

Boron neutron capture therapy (BNCT) can become an instrument for patients with malignant neoplasms of the rectum and colon. Here we evaluate the effectiveness of BNCT performed at the accelerator based epithermal neutron source at G. I.

Manganese Oxide Nanoparticles Inhibit the Growth of Subcutaneous U-87MG Glioblastoma Xenografts in Immunodeficient Mouse.

Our previous study demonstrated that manganese oxide nanoparticles (MnO NP) selectively destroyed U-87MG and U251 human glioblastoma cells in vitro. MnO NP were synthesized and studied by electron microscopy. Their antitumor properties were studied in vivo on the model of immunodeficient SCID mice with subcutaneous xenografts of U-87MG human glioblastoma.

Features of the Left Ventricular Functional Geometry in Patients with Myocardial Diseases with Varying Degrees of Systolic Dysfunction.

We revealed some features of the left ventricular functional geometry in patients with myocardial diseases with different degrees of left ventricular systolic dysfunction. A negative correlation was found between the spatio-temporal heterogeneity of the kinetics of the left ventricular wall during systole and ejection fraction in normal heart and in systolic dysfunction. The differences in the quantitative characteristics of the functional geometry between patients and normal subjects and between different groups of patients depended on the severityof left-ventricular systolic dysfunction.

PHARMACOLOGICAL CORRECTION OF MICROCIRCULATION IN RATS SUFFERED A TRAUMATIC BRAIN INJURY.

We investigated the action of mexicor on functional indices of erythrocytes and the structure of myocardial microcirculation in rats suffered from traumatic brain injury (TBI). At 3, 7 and 12 day after TBI we measured the concentration of 2,3-diphosphoglycerate (2,3-DPG), the degree of erythrocyte aggregation and their electrophoretic mobility (EPME) in the blood of rats, and also analyzed sections of left ventricular myocardium. During the first day after the TBI we observed a decrease in EPME, an increase of erythrocyte aggregation and an increase of 2,3-DFG concentration in erythrocytes compared with intact animals.

[USING MEXICOR TO CORRECT THE FUNCTIONAL PARAMETERS OF RED BLOOD CELLS IN RATS WITH TRAUMATIC BRAIN INJURY MODEL].

The effect of mexicor on the functional characteristics of erythrocytes under conditions of post-traumatic period has been studied on rats with traumatic brain injury (TBI) model. TBI leads to an increase in the aggregation of erythrocytes by 143% (p = 0.003), increases the concentration of malonic dialdehyde (MDA) by 40% (p = 0.

[Computer assisted modeling of endodontic access in mandibular and maxillary molars].

The paper describes the elaborated method of computer assisted manufacturing of plastic templates for optimal topography of endodontic access in molars according to CT and laser surface scanning data. The method was tested on extracted teeth, its use in clinical practice will greatly reduce the errors rate and minimize the hard tissues loss by endodontic assess creation.

[Modeling of disturbances in electrical and mechanical function of cardiomyocytes under acute ischemia].

The effect of acute myocardial ischemia on the electrical and mechanical function of cardiomyocytes was studied in the framework of a mathematical model of a single cardiomyocyte. Acute ischemia consequences were simulated via a combination of two factors--a reduction of intracellular ATP concentration and an increase in extracellular potassium concentration, which affect the kinetics of ATP-sensitive potassium current and other potassium currents. In accord with experimental data, ischemic models produce action potential shortening and diastolic depolarization, which reduce contractile, ability of cardiomyocytes, Utilizing a 'difference-current integral' approach, we assessed quantitative contribution of ionic currents to changes in the action potential generation during ischemic injuries.

[Method of root canal retreatment using computer simulation].

The paper describes a method of root canal retreatment that enables by means of CT data, laser surface scanning of teeth and a number of software to create plastic templates making possible successful retreatment with minimal risk of complications. The effectiveness of the method was evaluated in vitro on 10 extracted teeth. Application of the method in clinical practice enables the highest degree of accuracy and security of the procedure.

[Wilson-Konovalov disease in 3 sisters: a radical change in prognosis if timely diagnosed].

Wilson-Konovalov disease is a rare autosomal recessive genetic disorder in which copper accumulates in the liver, brain and other target organs. The paper describes a family case of the abdominal form of the disease in three sisters, the eldest of them died from fulminant liver failure at the age of 18 years. The second sister aged 16 years was diagnosed as having the disease at the stage of decompensated liver cirrhosis; her treatment with D-penicillamine resulted in complete disease remission.

[Electromechanical coupling in cardiomyocytes from transmural layers of guinea pig left ventricle].

The electrical and mechanical activity of heart ventricle cardiomyocytes is known to vary depending on the spatial location of cells in the wall, in particular, transmurally from the sub-endocardial layer to the sub-epicardial one. To investigate intracellular mechanisms of the functional heterogeneity of cardiomyocytes we developed mathematical models of the electromechanical coupling in cardiomyocytes from different transmural layers across the left ventricle (LV) wall of guinea pig. It is shown that the mechanisms of both direct linkages and feedback in the electromechanical coupling contribute to differences in both the shape and duration of action potential, and speed characteristics of contraction between isolated cardiac myocytes from the sub-endocardial and sub-epicardial layers.

[Morphogenesis of the structural changes of the internal organs in superinvasive opisthorchiasis].

The study of the model of superinvasive opisthorchiasis (SO) in Syrian hamsters and autopsy material obtained from patients with SO, it was shown that during the early period of pulmonary disease, the lungs underwent changes developing on the basis of delayed-type hypersensitivity reactions. In the chronic phase of SO, exudative and predominant productive reactions were revealed leading to the interstitial lung disease, fibrosing alveolitis. In the heart in SO, eosinophilic cell myocarditis was found with subsequent diffuse cardiosclerosis.

[Provisionality phenomenon in the histo- organo- and systemogenesis].

This review summarizes the results of the study of the provisionality phenomenon in the development of the organs of the urinary system (mesonephros, metanephros) in viviparous (human, rat) and oviparous (bird) amniotes, human organs of mixed origin (pituitary, ovary, stomodeum) reparative regeneration of supporting tissues (in humans and Syrian hamster), morphogenesis of primary hepatic cancer developing against the background of superinvasive opisthorchiasis. It is shown that during the development of tissues and organs, the stages of provisional and definitive histo- and organogenesis could be determined; saltatory mechanism is typical for embryonic organogenesis (formation of cartilaginous skeletal primordia, meso- and metanephrons) and foci of oncogenesis. Transformation zones of the type of tissue organization in the epithelium are located in the areas of contacts of the derivatives of different embryonic primordia.

Effect of cholinergic drugs on the activity of basic carboxypeptidases in rat nervous tissue.

Effects of a single administration of cholinergic drugs (arecoline, atropine, nicotine, mecamylamine) on the activity of carboxypeptidase H and of phenylmethylsulfonyl fluoride-inhibited carboxypeptidase, which are involved in metabolism of neuropeptides, were studied in brain parts and the adrenal glands of rats. The enzyme activities were determined fluorimetrically using specific inhibitors and substrates. In the majority of cases the enzyme activities decreased, and this decrease was retained for at least 72 h.

[Complicated forms of superinvasion opisthorchiasis: pathogenetic aspects].

Superinvasion opisthorchiasis is characterized by host growth retardation and weight reduction, which are due to the cleptoeffect of Opisthorchis felineus; metabolic disturbances, proliferative processes, accelerated collagen biosynthesis and biodegradation in the organs reflect parasite-host interactions as components of the system. The productive reactions of visceral pathology are initiated by the parasite's metabolites located in the liver, lung, heart, and testes.

[Effects of the neurogenesis stimulator Ro 25-6981 upon formation of spatial skill in adult rats depend on the term of its administration and the animals' ability to learn].

Effect of administration of selective N-methyl-D-aspartate (NMDA) receptor antagonist Ro 25-6981 on learning and memory in a dose which is known to stimulate neoneurogenesis was assessed in adult rats with different abilities to formation of spatial skills in different time periods after the antagonist injection. Wistar male rats were trained to find hidden platform in the Morris water maze for 5 consecutive days. Rats' learning ability for spatial skill formation was evaluated depending on platform speed achievements.

[Mathematical modelling in physiology].

The article illustrates the method of mathematical modelling in physiology as a unique tool to study physiological processes. A number of demonstrated examples appear as a result of long-term experience in mathematical modelling of electrical and mechanical phenomena in the heart muscle. These examples are presented here to show that the modelling provides insight into mechanisms underlying these phenomena and is capable to predict new ones that were previously unknown.

[The problem of myocardial heterogeneity].

In this paper, we present a review of modern data on the myocardial heterogeneity. Data obtained by different authors as well as our own results clearly show that cardiac muscle is an essentially heterogeneous system at every level of organization: from molecular to organ. Cardiomyocyte function in the myocardial system is closely coordinated: both temporarily and spatially, with complex motion of cardiac chamber walls.

[Contribution of cooperative mechanisms of the thin filament activation to the myocardium contractile function. Assessment by a mathematical model].

We applied a mathematical model to compare the contributions to the myocardium mechanical activity of two a priori feasible variants of the cooperative effect of myosin cross-bridges on the calcium activation of sarcomere thin filaments. One of these variants implies that cross-bridge cooperative influence on the troponin C affinity to calcium is localized within the same functional cluster A7TmTn where the cross-bridge is attached. The second variant is based on the assumption that cross-bridges may affect the affinity of troponin C to calcium in other functional clusters A7TmTn as well (so that the nearer to the cross-bridge a cluster lies, the stronger the cross-bridge affects the affinity of CaTnC complex in this cluster).

[Spatio-temporal heterogeneity of human left ventricle contractions in norm and under ischemic heart disease].

2D-ultrasonic images of the left ventricle are analyzed and spatio-temporal heterogeneity of regional wall motion is characterized quantitatively in norm and in ischemic heart disease. Negative correlation between regional heterogeneity and global ejection fraction is revealed. Regional heterogeneity is shown to increase significantly in a group of patients as compared to healthy people.

[Mechanisms of electromechanical function disturbances in cardiomyocytes overloaded with calcium. The theoretical study].

Arrhythmias and mechanical disturbances are simulated in a mathematical model of cardiomyocyte electromechanical activity. The simulated pattern is similar to that observed for acute heart failure associated with calcium overloading of myocardium cells. Special attention was paid to the calcium overloading resulting from the reduced Na+,K+ pump activity.

[Mathematical models for the study of electromechanical and mechanoelectrical coupling in the myocardium].

Mathematical models have been developed to describe interactions of electrical, mechanical and chemical processes in cardiomyocytes. The models simulate wide range of experimental data on excitation-contraction coupling and, more importantly, on mechanoelectric feedback in heart muscle. The model results clearly show that mechano-dependence of intracellular calcium handling due to cooperative effects of contractile proteins activation plays a key role in cardiac mechanoelectric coupling.