The Hemostatic System in Newborns and the Risk of Neonatal Thrombosis.

Newborns are the most vulnerable patients for thrombosis development among all children, with critically ill and premature infants being in the highest risk group. The upward trend in the rate of neonatal thrombosis could be attributed to progress in the treatment of severe neonatal conditions and the increased survival in premature babies. There are physiological differences in the hemostatic system between neonates and adults.

Hydroxychloroquine in obstetric antiphospholipid syndrome: rationale and results of an observational study of refractory cases.

Background: The current recommended therapy of obstetric antiphospholipid syndrome (APS) is a long-term anticoagulant therapy that affects the final event, namely, when the thrombosis has already occurred. Unfortunately, this schedule is not always effective and fails despite the correct risk stratification and an adequate adjusted dose.

Materials And Methods: From 2013 to 2020 we observed 217 women with antiphospholipid antibodies and obstetric morbidities who were treated with conventional treatment protocol (aspirin low doses ± LMWH).

Determination of Bifidobacterial Carbohydrate Utilization Abilities and Associated Metabolic End Products.

Bifidobacteria are able to utilize a diverse range of host-derived and dietary carbohydrates, the latter of which include many plant-derived oligo- and polysaccharides. Different bifidobacterial strains may possess different carbohydrate utilization abilities. These metabolic abilities can be studied using classical bacterial growth assessment methods, such as measurement of changes in optical density or acidity of the culture in the presence of the particular carbohydrate to generate growth and acidification curves, respectively.

Riboflavin Biosynthesis and Overproduction by a Derivative of the Human Gut Commensal subsp. ATCC 15697.

Riboflavin or vitamin B is the precursor of the essential coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Despite increased interest in microbial synthesis of this water-soluble vitamin, the metabolic pathway for riboflavin biosynthesis has been characterized in just a handful of bacteria. Here, comparative genome analysis identified the genes involved in the biosynthetic pathway of riboflavin in certain bifidobacterial species, including the human gut commensal subsp.

Development of Biosensors for Detection of Diacetyl.

Some secondary metabolites of fermentative bacteria are desired compounds for the food industry. Examples of these compounds are diacetyl and acetaldehyde, which are produced by species of the lactic acid bacteria (LAB) family. Diacetyl is an aromatic compound, giving the buttery flavor associated with dairy products, and acetaldehyde is the compound responsible for the yogurt flavor and aroma.

Ampicillin-treated Lactococcus lactis MG1363 populations contain persisters as well as viable but non-culturable cells.

Lactococcus lactis is used as cell-factory and strain selections are regularly performed to improve production processes. When selection regimes only allow desired phenotypes to survive, for instance by using antibiotics to select for cells that do not grow in a specific condition, the presence of more resistant subpopulations with a wildtype genotype severely slows down the procedure. While the food grade organism L.

Unusual thrombosis or pregnancy complications associated to ovarian cancers: two clinical cases.

Aim: Using illustrative cases of two patients with ovarian cancer who developed unusual thrombosis in pregnancy, to describe the difficulties in diagnosing and managing such patients.

Methods: We present the analysis of two cases of thrombosis of unusual localization (central artery of the retina and transverse and sigmoid sinus thrombosis) during pregnancy in women with hidden ovarian cancer.

Results: In both cases, the symptomatic of unususal thrombosis was interpreted as a manifestation of eclampsia, leading to a choice of non-optimal obstetric tactics on a background of hidden ovarian cancer, which in both cases were diagnosed only postpartum.

Engineering Lactococcus lactis for the production of unusual anthocyanins using tea as substrate.

Plant material rich in anthocyanins has been historically used in traditional medicines, but only recently have the specific pharmacological properties of these compounds been the target of extensive studies. In addition to their potential to modulate the development of various diseases, coloured anthocyanins are valuable natural alternatives commonly used to replace synthetic colourants in food industry. Exploitation of microbial hosts as cell factories is an attractive alternative to extraction of anthocyanins and other flavonoids from plant sources or chemical synthesis.

Metabolic engineering and synthetic biology employing Lactococcus lactis and Bacillus subtilis cell factories.

Metabolic engineering and synthetic biology approaches have prospered the field of biotechnology, in which the main focus has been on Escherichia coli and Saccharomyces cerevisiae as microbial workhorses. In more recent years, improving the Gram-positive bacteria Lactococcus lactis and Bacillus subtilis as production hosts has gained increasing attention. This review will demonstrate the different levels at which these bacteria can be engineered and their various application possibilities.

[BORDERLINE OVARIAN TUMORS: CURRENT ISSUES AND REHABILITATION PROBLEMS].

Objective of the review - to demonstrate the topicality of borderline ovarian tumors; to analyze the current state of the rehabilitation in patients who undergo specific antitumor treatment. Borderline ovarian tumors (BOT) more frequently occur in young patients (below age 40 years); herewith five-year survival rate exceeds 95%. The cumulative data on pathogenesis, clinical progression, therapy and rehabilitation possibilities together with epidemiological features gave an opportunity to take a fresh look not only at the disease itself but at the necessity of rehabilitation of patients and quality of their life.

[CERVICAL CANCER REHABILITATION: A PROSPECTIVE STUDY].

Aim - to evaluate the rehabilitation program impact on the quality of life of оncogynecological patients and to introduce this approach in clinical practice. The study included93 reproductive-aged women, who underwent the cervical cancer treatment. Patients were divided into two groups: the experimental group, which received rehabilitation, and the control group.

Further Elucidation of Galactose Utilization in MG1363.

Since the 1970s, galactose metabolism in has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP- dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTS exists.

[MODERN VIEW ON ETIOPATHOGENESIS AND NEW OPPORTUNITIES FOR DIAGNOSIS OF UTERINE FIBROIDS].

Uterine fibroids remain a burning issue for modern gynecology and cause suffering to women, significantly worsening their quality of life. This article includes the latest information about etiology, risk factors, pathobiochemical, genetic and immunological features of pathogenesis, clinical findings, early detection of fibroids and sarcoma differential diagnostic criteria by ultrasonography and MRI. Foreign publications form the basis of the given article, as well as domestic ones.

[ENDOMETRIOSIS: A NEW APPROACH TO ETIOLOGY AND PATHOGENESIS (REVIEW)].

Endometriosis is a dyshormonal immune-dependent genetically determined disease, which appears as an endometrioid tissue that grows outside the uterine. Endometriosis is one of the most urgent problems of medicine. To date, new concepts of the endometriosis etiology and pathogenesis have been developed, but, despite their abundance, there is no unified theory.

[MODERN APPROACHES TO DIAGNOSIS AND TREATMENT OF TUMORS OF FEMALE GENITALS OF CHILDREN AND ADOLESCENTS (REVIEW)].

The article discusses the features of epidemiology, morphology, clinical course and modern principles of diagnostics and treatment of tumors of female genitals of children and adolescents. Special attention is paid to ovarian germ cell tumors. The use of immunohistochemical and tumor markers enables the differential diagnosis among malignant ovarian germ cell tumors.

Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363.

subsp. strains typically carry many dairy niche-specific adaptations. During adaptation to the milk environment these former plant strains have acquired various pseudogenes and insertion sequence elements indicative of ongoing genome decay and frequent transposition events in their genomes.

The Evolution of gene regulation research in Lactococcus lactis.

Lactococcus lactis is a major microbe. This lactic acid bacterium (LAB) is used worldwide in the production of safe, healthy, tasteful and nutritious milk fermentation products. Its huge industrial importance has led to an explosion of research on the organism, particularly since the early 1970s.

Transcriptome analysis shows activation of the arginine deiminase pathway in Lactococcus lactis as a response to ethanol stress.

This paper describes the molecular response of Lactococcus lactis NZ9700 to ethanol. This strain is a well-known nisin producer and a lactic acid bacteria (LAB) model strain. Global transcriptome profiling using DNA microarrays demonstrated a bacterial adaptive response to the presence of 2% ethanol in the culture broth and differential expression of 67 genes.

[Evaluation of the cytolytic effect of tonic with ginseng extract].

Experimental evaluation of the cytolytic effect of prolonged use of tonic drink with ginseng extract by determining the activity of enzymes, markers of cytolysis (alanine aminotransferase, aspartate aminotransferase, creatine kinase and lactate dehydrogenase (LDH)) has been carried out. The study was performed on male rats Wistar (n=15, body weight of 461±23 g), to which tonic drink (containing 46 mg/L panaxosides) was administered in the dose 15 mg/kg body weight using a pharyngeal tube. The introduction was carried out three times daily for 21 days.

Regulation of Cell Wall Plasticity by Nucleotide Metabolism in Lactococcus lactis.

To ensure optimal cell growth and separation and to adapt to environmental parameters, bacteria have to maintain a balance between cell wall (CW) rigidity and flexibility. This can be achieved by a concerted action of peptidoglycan (PG) hydrolases and PG-synthesizing/modifying enzymes. In a search for new regulatory mechanisms responsible for the maintenance of this equilibrium in Lactococcus lactis, we isolated mutants that are resistant to the PG hydrolase lysozyme.

Microbial bet-hedging: the power of being different.

Bet-hedging is an evolutionary theory that describes how risk spreading can increase fitness of a genotype in an unpredictably changing environment. To achieve risk spreading, maladapted phenotypes develop within isogenic populations that may be fit for a future environment. In recent years, various observations of microbial phenotypic heterogeneity have been denoted as bet-hedging strategies, sometimes without sufficient evidence to support this claim.

Bet-hedging during bacterial diauxic shift.

When bacteria grow in a medium with two sugars, they first use the preferred sugar and only then start metabolizing the second one. After the first exponential growth phase, a short lag phase of nongrowth is observed, a period called the diauxie lag phase. It is commonly seen as a phase in which the bacteria prepare themselves to use the second sugar.

Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.

Green fluorescent protein (GFP) offers efficient ways of visualizing promoter activity and protein localization in vivo, and many different variants are currently available to study bacterial cell biology. Which of these variants is best suited for a certain bacterial strain, goal, or experimental condition is not clear. Here, we have designed and constructed two "superfolder" GFPs with codon adaptation specifically for Bacillus subtilis and Streptococcus pneumoniae and have benchmarked them against five other previously available variants of GFP in B.