Extensively drug-resistant Acinetobacter baumannii belonging to the international clonal lineage I in a Russian burn intensive care unit.

The high incidence of mortality in patients with severe burns can be attributed to bloodstream infections caused by drug-resistant microorganisms. Multilocus variable-number tandem repeat analysis (MLVA), multilocus sequence typing (MLST) and class 1 integron PCR amplification were performed to investigate an extensively drug-resistant Acinetobacter baumannii (XDR-AB) strain recovered from a blood culture of a patient admitted to a burn intensive care unit in St Petersburg (Russian Federation). This case study describes an XDR-AB strain of multilocus sequence type ST231 with a blaGES-12 gene cassette encoding a very potent ceftazidimase located inside of a composite class 1 integron.

Genetic stability and worldwide dissemination of integron insert encoding trimethoprim resistance.

A hospital-based surveillance study of class 1 integron-positive Acinetobacter isolates in the Urals and Siberia (Russia) demonstrated that dihydrofolate reductase gene (dfrA5) was located in a functional gene cassette insertion driven from a promoter of a weak type. This insertion is an example of the integron-associated trimethoprim resistance disseminated worldwide from the free-living animals to human. Despite the long-term persistence as an integron insert any loss or modifications in the nucleotide sequence especially at attC site was not observed.

[Class 1 integron-associated promoters for gene cassette transcription].

In the present paper nucleotide sequences of inner parts of class 1 integrons (natural vectors of cloning and expression of bacterial genes) were determined for the Acinetobacter strains isolated in the Russian medical centres. The similar compositions of inserted gene cassettes are known among the strains disseminated worldwide as minimum as 10 years. Promoter regions directed gene cassettes transcription appeared to be stable parts of integron sequences and mutational shift toward more strengthened type was not revealed.

[ISAba1 insertion sequence in genome of epidemically relevant Acinetobacter strains].

Aim: To detect presence of insertion sequence ISAba1 in genome of strains of Acinetobacter baumanii group isolated in hospitals.

Materials And Methods: Amplification by polymerase chain reaction with oligonucleotide primers specific for ISAba1 region with promoter sequence was performed.

Results: Insertion element ISAba1 was detected in A.

[Emergence of the integron-positive multi-drug resistant strain of Acinetobacter baumanii in Russian hospitals].

Aim: To detect the integron-positive strains among nosocomial Acinetobacter spp. and to determine their relationship on the genotype level.

Materials And Methods: Amplification by polymerase chain reaction using primers specific to sequences of the class 1 and 2 intergrons on the genomic DNA template followed by restriction fragments length polymorphism analysis as well as RAPD-genotyping of the integron-positive strains were performed.

[The effect of silver sulfadiazine on Acinetobacter baumannii strains isolated in burn units].

The genotype structure and silver sulfadiazine (SDS) resistance of a number of Acinetobacter baumanii strains that circulated for a prolonged period of time in burn UCUs were studied. The most resistant strain (SDS MIC 50 mcg/ml) contained a class 1 integron with the gene of sulfonamides resistance (sul1) in its genome. Possible reasons for selection of the multiple resistance among Acinetobacter spp.

[Genetic variety of Acinetobacter baumannii in an intensive care unit for burn patients].

The genetic structure of A. baumannii hospital isolates, formed in the course of 2002 - 2004 in an intensive care unit for burn patients (St. Petersburg) was studied.

[Integrons of bacteria isolated from aquatic environment].

Bacteria of the genus Pseudomonas, isolated from the water of the lakes Shira and Itkul (Republic of Khakassia, Russia) were shown to contain integrons of class 1 with gene cassettes, contained in the variable segment (sized 1 and 1.3 kb), were shown. Out of three detected integrons only one integron (in P.

[Terminology of a computer network: notes of a non-epidemiologist].

In the development of discussion on epidemiological terms containing definitions "epidemic" and "epidemiological" variants of these terms as used in electronic documents placed on Internet sites are considered. Both most common combinations and contradictions in the use of some terminological constructions are presented.

[PCR genotyping of Acinetobacter hospital isolates].

A total of 13 Acinetobacter baumannii-13TU isolates obtained from patients of the Perm regional clinical hospital during the period of 1 year, were genotyped in the polymerase chain reaction (PCR) with universal primers. Acinetobacter cultures could not be distinguished by phenotypic tests and antibiogram and were resistant to B-lactams and gentamicin. According to the results of amplification the obtained isolates could be subdivided into two groups (with 6 and 7 respectively).

[Intracellular accumulation of monomer precursors of polyphosphates and polyhydroxyalkanoates from Acinetobacter calcoaceticus and Escherichia coli].

The formation of polyhydroxyalkanoates granules in anaerobically grown Escherichia coli cells was found to be preceded by the intracellular accumulation of carbonic acids (predominantly, acetic acid), amounting to 9% of the cytosol. The intracellular concentration of acidic metabolites increased after the lyophilization of the bacterial biomass and decreased after its long-term storage (3.5-13.