Ultrafast CEST line scanning as a method to quantify mutarotation kinetics.

The process of mutarotation of sugars caused by a balanced reaction between their corresponding α and β isomers, has been known for almost 200 years. Still, it remains essential in modern biochemical research, as enzymatic reactions catalyzed by mutarotases are crucial for various pathways in the energy metabolism. In our study a fast magnetic resonance technique based on chemical exchange saturation transfer (CEST) line scanning (LS) was implemented as a method to measure mutarotation kinetics on a 9.

In vivo detection of carnosine and its derivatives using chemical exchange saturation transfer.

Purpose: To detect carnosine, anserine and homocarnosine in vivo with chemical exchange saturation transfer (CEST) at 17.2 T.

Methods: CEST MR acquisitions were performed using a CEST-linescan sequence developed in-house and optimized for carnosine detection.

PEAKIT: A Gaussian Process regression analysis tool for chemical exchange saturation transfer spectra.

Chemical Exchange Saturation Transfer (CEST) is a powerful technique for metabolic imaging, capable of exploring concentrations in the μM to mM range. However, extracting quantitative information from Z-spectra can be challenging due to the non-CEST contributions present and the limited knowledge about the exchanging pools. The PEAKIT tool is proposed as an alternative approach to quantifying CEST peaks, which requires no prior assumptions about the frequency offset or the underlying shape of the baseline.