Development and validation of a bioanalytical method for the quantification of CHF6550 and its metabolite (CHF6671) in rat plasma and lung homogenate using LC-MS/MS.

A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for accurate determination of CHF6550 and its main metabolite in rat plasma and lung homogenate samples. All biological samples were prepared by simple protein precipitation method using deuterated internal standards. The analytes were separated on a HSS T3 analytical column with 3.

Hyperbaric oxygen therapy as adjuvant therapy for odontogenic necrotizing myositis: A case report.

In a young man affected by skin soft tissue infections complicated with myositis, the use of hyperbaric oxygen treatment as an adjuvant therapy to surgical debridement and antibiotic therapy could improve management and prognosis.

Development and validation of bioanalytical methods for LNA-i-miR-221 quantification in human plasma and urine by LC-MS/MS.

LNA-i-miR-221, a 13-mer oligonucleotide, has proved favorable efficacy and safety profiles in the preclinical studies, leading to being approved for use in clinical trials by regulatory authorities. The objective of this study was to develop and validate LC-MS/MS methods to quantify LNA-i-miR-221 in human plasma and urine. Chromatographic separation was performed with a gradient system on HALO C18 column using hexafluoro-2-propanol/triethylamine buffer and methanol as mobile phase.

Impact of COVID-19 epidemic on maxillofacial surgery in Italy.

Maxillofacial departments in 23 surgical units in Italy have been increasingly involved in facing the COVID-19 emergency. Elective surgeries have been progressively postponed to free up beds and offer human and material resources to those infected. We compiled an inventory of 32 questions to evaluate the impact of the SARS-COV2 epidemic on maxillofacial surgery in 23 selected Italian maxillofacial departments.

Development and validation of a bioanalytical method for quantification of LNA-i-miR-221, a 13-mer oligonucleotide, in rat plasma using LC-MS/MS.

LNA-i-miR-221, a 13-mer oligonucleotide, is a new miR-221 inhibitor that could be used as a novel drug for multiple myeloma. Herein, an ion-pair reversed phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantification of LNA-i-miR-221 in rat plasma. Plasma samples were prepared with an initial phenol/chloroform/isoamyl alcohol liquid-liquid extraction followed by a solid phase extraction.

Development and validation of a highly sensitive gradient chiral separation of pramipexole in human plasma by LC-MS/MS.

Aim: Development of a high-sensitivity chiral LC-MS/MS method was required to evaluate a combination of pramipexole (S-PPX) and its enantiomer dexpramipexole (R-PPX) in a proposed clinical trial. The previously available methods suffered from low sensitivity for the (S)-enantiomer in the presence of the more abundant (R)-enantiomer. Based on the projected dosing regimen in the clinical trial, a 5000-fold improvement in sensitivity was required for the (S)-enantiomer.

Osteonecrosis of the jaws in patients assuming oral bisphosphonates for osteoporosis: a retrospective multi-hospital-based study of 87 Italian cases.

Background: Bisphosphonates (BPs) are currently the chief drugs for the prevention/treatment of osteoporosis; one of their adverse effects is the osteonecrosis of the jaw (BRONJ). The primary endpoints of this multi-center cross-sectional study are: i) an observation of the clinical features of BRONJ in 87 osteoporotic, non-cancer patients; and ii) an evaluation of their demographic variables and comorbidities.

Methods: 87 BRONJ patients in therapy for osteoporosis with BPs from 8 participating clinical Italian centers were consecutively identified and studied.

Resective surgical approach shows a high performance in the management of advanced cases of bisphosphonate-related osteonecrosis of the jaws: a retrospective survey of 347 cases.

Purpose: The aim of this study was to evaluate the results of the surgical treatment of bisphosphonate-related osteonecrosis of the jaw (BRONJ) in a large cohort.

Materials And Methods: A retrospective cohort multicenter study was designed. Patients were enrolled if they were diagnosed with BRONJ and received operative treatment.

Plasma protein binding and blood-free concentrations: which studies are needed to develop a drug?

Introduction: The plasma protein binding of drugs and metabolites is known to influence their pharmacokinetics and, therefore, their effects. Evaluating the extent and the linearity of protein binding is an essential piece of information that has to be generated during drug development. Blood cell partitioning has a similar relevance.

Surgery-triggered and non surgery-triggered Bisphosphonate-related Osteonecrosis of the Jaws (BRONJ): A retrospective analysis of 567 cases in an Italian multicenter study.

Invasive local procedures are often reported in clinical history of patients suffering from Bisphosphonates-Related Osteonecrosis of the Jaws (BRONJ) but over 40% of spontaneous forms have been also described in literature. We compared age, gender, underlying bone disorders, bisphosphonate therapy, clinical features and surgical outcome of 205 cases (36.2%) of BRONJ non surgery-triggered (group 1) with 362 (63.

Synovial sarcoma of the neck in a child: a multidisciplinary approach.

Synovial sarcoma (SS) is the most common nonrhabdomyosarcomatous soft tissue sarcoma in childhood, but the head-neck site accounts for less than 5% of cases. The authors report a 10-year-old boy with SYT-SSX1 positive left parapharyngeal SS, resistant to front-line VAIA chemotherapy, who obtained a good partial response by salvage regimen (I(3)VE + CEV + I(3)VE) and local radiotherapy, so a complete surgical resection could be performed. The complete remission was subsequently consolidated by ablative high-dose chemotherapy, followed by autologous stem cell reinfusion.

[Membrane platelet fatty acids: biochemical characterisation of the ischemic cardiovascular disease, characteristics of the paediatric age, through an artificial neural network interpretation].

During the last 30 years, a huge research has been performed about the markers involved in the atherogenetic process and in the fatty acid thrombogenetic effect. More recently many studies have been made on the biochemical aspects of the saturated fatty acids with respect to the cardiovascular risk, in particular, the Stearic Acid (SA). Because of the peculiar characteristic of the platelets in the control of the thrombogenetic risk, we have investigated the platelet fatty acid profile in three groups of subjects: supposed healthy (n = 60), ischemic (n = 50), young children (n = 118).

[Adenophlegmons of the neck area. A case report and review of the literature].

Adenopathies in general and cervicofacial adenopathies in particular are a pathology that can affect both medical and surgical disciplines. They may be found in both acute and chronic disorders, in children and in adults, and in local or systemic forms with benign or malignant pathogenesis. It is clear that the differential diagnosis and consequently the therapeutic approach may be complicated at times by the variety of pathological processes.

[Dermoid cysts of the oral floor. A clinical case report and review of the literature].

The authors report two cases of dermoid cysts of the oral floor. The incidence of this pathology in this region is difficult to assess due to the rarity of reported cases. In particular, dermoid cysts appear to derive from the inclusion of epithelial cells on the median line in the fusion point between the first and second branchial arch.

[Fractures of the temporo-zygomatic arch. The clinical and therapeutic aspects].

Fractures of the orbito-maxillo-zygomatic complex are often associated with fractures of the temporo-zygomatic arcade. After a brief discussion, the authors describe a simple method to support the fractured arcade in the event of inadequate containment and/or the precarious stability of the latter.

[Surgical experience of complex fractures of the middle third of the facial skeleton treated without an intermaxillary block].

Having illustrated the functional and anatomical aspects of the upper jaw and the importance of the anterior and posterior pillars as load-bearing structures for the middle third of face, the paper reports a series of fractures of the upper jaw treated with plaque osteosynthesis without an intermaxillary block. The functional and esthetic advantages are underlined.

[Reconstructive surgery of the ventral surface of the mobile tongue. The authors' personal experience].

The neoplastic pathology of the cervico-cephalic district in an advanced stage of the disease often of necessity involves radical, mutilating operations. Following some brief remarks, personal experience in the reconstructive surgery of the ventral face of the mobile tongue and the oral floor is presented.

[The problem of the mandible in surgery of tonsillar tumors: proposal of a technique].

The Authors report a technique of a conservative transmandibular approach for the surgical treatment of tonsillar region malignancies. The procedure is based upon the preparation of a vascular mandibular flap through two osteotomies, which are performed, the first above the mandibular foramen and the second below the mental foramen. The main advantages of present technique are: lip-chin-splitting is avoided and, due to the preservation of mandibular vascular and nervous supply, it is possible to accomplish, without risk, postoperative radiotherapy.

[Multiple lipomatosis or Launois-Bensaude disease. Technics for surgical treatment in our clinical experience].

Only 200 cases of this extremely rare disease of unknown etiology have been reported in the literature. The paper examines the various surgical techniques and describes two cases operated by the authors.

[Carcinoma of the oral cavity: integrated treatment].

An integrated chemoradio-surgical technique for treating carcinoma of the oral cavity is described. The results obtained are particularly promising. Longer-term follow-ups are needed for final assessment.

[Intra-arterial chemotherapy. Presentation of a method to insure patency of the catheter].

Following some brief remarks on the main techniques of antineoplastic chemotherapy, a simple technique for guaranteeing patency of the endarterial catheter is presented. Using a special microchannel infusor, it was possible to prevent catheter obstruction in 100% of the cases treated.

[Clinical and therapeutic considerations on a case of hemangiopericytoma of the cheek].

The paper reports on a case of recurrent hemangiopericytoma of the cheek, discusses the results of hyperthermia combined with intra-arterial chemotherapy after the postoperative recurrence and draws significant conclusions about the therapeutic approach to this type of tumour.