Rapid Metabolic Recovery of Donor Circulatory Death Liver Graft Using Whole Blood Perfusion: A Pig Study.

Unlabelled: Ex vivo perfusion technology has been actively developed to solve the problem of severe donor shortage. In this study, the ex vivo metabolic characteristics of porcine donation after circulatory death (DCD) liver in short-term perfusion using whole or diluted blood were compared with those of the in vivo transplanted state to evaluate their initial response to resuscitation.

Methods: The porcine DCD model was constructed by clamping the thoracic aorta.

Roles of Atomic Nitrogen/Hydrogen in GaN Film Growth by Chemically Assisted Sputtering with Dual Plasma Sources.

The growth of sputtered GaN at low temperature is strongly desired to realize the dissemination of low-cost GaN high electron mobility transistor devices for next-generation communication technology. In this work, the roles of atomic nitrogen (N)/hydrogen (H) in GaN film growth on AlN/sapphire substrates by chemically assisted dual source sputtering are studied at a low growth temperature of 600 °C under a pressure of 2 Pa using vacuum ultraviolet absorption spectroscopy. The lateral growth was strongly enhanced with an appropriate H/N flux ratio of 1.

Development of a Simple and Active Shunt System in the Anhepatic Stage for Surgical Training of Orthotopic Liver Transplantation.

Background: A pig model has been commonly used for technical training for clinical liver transplantation (LT). However, as the healthy pigs have no shunt bypassing the portal vein (PV), it is necessary to complete LT within 30 minutes after shutting off the PV flow. While a model that uses an ex vivo shunt system has been used to alleviate the constraints of the anhepatic phase, it has been often difficult to keep sufficient blood flow rate and prevent the intestinal congestion because the blood vessels were occluded easily with the suction pressure by using the conventional shunt system.

Continuous Resuscitation for Porcine Liver Transplantation From Donor After Cardiac Death.

Background: To solve the serious donor shortage, the demand is increasing for developing a new method to use the marginal donors, including donors after cardiac death (DCD). Continuous machine perfusion from ex vivo to in situ is a novel technique to overcome warm ischemia during organ grafting as an ischemia-free transplantation. Herein, we tested orthotopic and heterotopic ischemia-free liver transplantation in pigs and evaluated the perfusion characteristics of DCD grafts from ex vivo preservation to implantation.

Reduction of Warm Ischemia Using a Thermal Barrier Bag in Kidney Transplantation: Study in a Pig Model.

Background: With recent advances in surgical technologies, minimally invasive endoscopic and robot-assisted surgical procedures have been introduced. However, prolonged warm ischemic time of the kidneys remains a concern after the organ is removed from a donor and during transplantation into a recipient. We developed a Thermal Barrier Bag (TBB) to prevent warm ischemia during transplantation.