Dilemma of ST-Segment Elevation and Subarachnoid Hemorrhage: A Case Report and Literature Review.

Acute ST-segment elevation myocardial infarction (STEMI) occurs due to occlusion of one or more coronary arteries causing myocardial injury. It is a medical emergency and requires prompt diagnosis and intervention. Transient ST-segment elevation can occur due to coronary vasospasm, and their association has been reported with subarachnoid hemorrhage.

Preparation and application of hydroxypropyl methylcellulose blended with beeswax and essential oil edible coating to enhance the shelf life of sweet cherries.

The study involved preparing and applying edible nano-emulsion coatings containing hydroxypropyl methylcellulose (HPMC), beeswax (BW), and essential oils (thyme, cinnamon, clove, and peppermint) onto sweet cherries. The application was conducted at 4 °C, and the coated cherries were stored for 36 days. This research examines synthesized nano-emulsions physicochemical properties and antibacterial and antifungal activities (C, C, and C).

Transformative role of educational funding in shaping national development across SAARC countries in the 21st century: A panel NARDL approach.

Since the beginning of this century, there has been evidence of a rise in educational funding among the South Asian Association for Regional Cooperation (SAARC). However, there has been a decline in recent years despite South Asia being a highly populated and poverty-ridden region. Thus, the present study comes to assess how well the countries are doing in relation to the effect of educational funding on national development indicators, namely economic growth, human capital development, and the unemployment rate among the SAARC countries in the 21st century using Panel Nonlinear Autoregressive Distributed Lag (PNARDL) model formulated in Salisu and Isah (2017).

How to eradicate polio in Pakistan: Insights from community health workers.

Pakistan, along with Afghanistan, is one of two countries where wild poliovirus is still endemic. Frontline workers (FLWs) are the staff most intimately familiar with both implementation challenges and community context. Harnessing their expertise may be a way to improve the community-polio program interface, which has been a persistent and shifting challenge in polio-endemic areas of both countries.

Exploring the interrelationship among health status, CO emissions, and energy use in the top 20 highest emitting economies: based on the CS-DL and CS-ARDL approaches.

Carbon dioxide emissions (COe) which is caused by energy use contributes to the global average surface temperature increase by 1.5 °C as compared to the mid-1800s which is causing a certain change in the climate and becoming an adverse effect on health and economy. The relationship between health status, COe, and energy use has yet to be thoroughly investigated in the top 20 highest emitting economies.

Ramification of healthcare expenditure on morbidity rates and life expectancy in the association of southeast asian nations countries: A dynamic panel threshold analysis.

Objective: This study has investigated the impact of healthcare expenditure on life expectancy and morbidity rates in the Association of Southeast Asian Nations to analyse whether health expenditure remains a critical component of improving health status and to determine the threshold value at which health strategy becomes cost-effective from 2000 to 2019.

Methods: The techniques employed include the Dynamic Panel Threshold model advanced by Seo et al. (2019) to implement Seo and Shin's (2016) proposed first-differenced generalised method of moments (GMM) estimation.

The nonlinearity and nonlinear convergence of CO emissions: Evidence from top 20 highest emitting countries.

Carbon dioxide (CO) is the most prevalent greenhouse gas that triggers climate change, which in turn leads to catastrophic effects on trade, business, human health, and other areas. Understanding the characteristics and tendency of CO emissions will improve policy making and mitigation strategies. Understanding the linearity or nonlinearity and convergence or divergence of CO emissions is essential for selecting appropriate modeling techniques and for designing reliable policies.

Association of Trace Metals in Patients with Schizophrenia.

Objective: To compare the serum concentrations of copper, iron and zinc in schizophrenic patients with healthy individuals.

Study Design: Cross-sectional comparison study.

Place And Duration Of Study: Department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan.

Coagulation Gene Expression Profiling in Infants With Necrotizing Enterocolitis.

Objectives: Coagulopathy and mesenteric thrombosis are common in premature neonates with necrotizing enterocolitis (NEC). This pilot study aimed to investigate the hypothesis that there are changes in the gene expression related to the coagulation and anticoagulation systems in NEC.

Methods: Consecutive neonates (n = 11) with NEC (Bell stages 2-3) were recruited.

Interpreting Heterogeneity in Response of Cells Expressing a Fluorescent Hydrogen Peroxide Biosensor.

Fluorescent, genetically encoded sensors of hydrogen peroxide have enabled visualization of perturbations to the intracellular level of this signaling molecule with subcellular and temporal resolution. Ratiometric sensors hold the additional promise of meaningful quantification of intracellular hydrogen peroxide levels as a function of time, a longstanding goal in the field of redox signaling. To date, studies that have connected the magnitudes of observed ratios with peroxide concentrations have either examined suspensions of cells or small numbers of adherent cells (∼10).

Exploring nicotinamide cofactor promiscuity in NAD(P)H-dependent flavin containing monooxygenases (FMOs) using natural variation within the phosphate binding loop. Structure and activity of FMOs from sp. BR and NF13.

Flavin-containing monooxygenases (FMOs) catalyse asymmetric oxidation reactions that have potential for preparative organic synthesis, but most use the more expensive, phosphorylated nicotinamide cofactor NADPH to reduce FAD to FADH prior to formation of the (hydro)peroxy intermediate required for substrate oxygenation. A comparison of the structures of NADPH-dependent FMO from (mFMO) and SMFMO from , which is able to use both NADPH and NADH, suggested that the promiscuity of the latter enzyme may be due in part to the substitution of an Arg-Thr couple in the NADPH phosphate recognition site in mFMO, for a Gln-His couple in SMFMO (Jensen et al., 2012, , 13, 872-878).

Mutations of an NAD(P)H-dependent flavoprotein monooxygenase that influence cofactor promiscuity and enantioselectivity.

The flavoprotein monooxygenase (FPMO) from Stenotrophomonas maltophilia (SMFMO, Uniprot: B2FLR2) catalyses the asymmetric oxidation of thioethers and is unusual amongst FPMOs in its ability to use the non-phosphorylated cofactor NADH, as well as NADPH, for the reduction of the FAD coenzyme. In order to explore the basis for cofactor promiscuity, structure-guided mutation of two residues in the cofactor binding site, Gln193 and His194, in SMFMO were performed in an attempt to imitate the cofactor binding site of the NADPH-dependent FMO from Methylophaga aminisulfidivorans sp. SK1 (mFMO), in which structurally homologous residues Arg234 and Thr235 bind the NADPH 2'-ribose phosphate.

Genome sequence of Stenotrophomonas maltophilia PML168, which displays Baeyer-Villiger monooxygenase activity.

Stenotrophomonas maltophilia PML168 was isolated from Wembury Beach on the English Coast from a rock pool following growth and selection on agar plates. Here we present the permanent draft genome sequence, which has allowed prediction of function for several genes encoding enzymes relevant to industrial biotechnology, including a novel flavoprotein monooxygenase.

A flavoprotein monooxygenase that catalyses a Baeyer-Villiger reaction and thioether oxidation using NADH as the nicotinamide cofactor.

A gene from the marine bacterium Stenotrophomonas maltophilia encodes a 38.6 kDa FAD-containing flavoprotein (Uniprot B2FLR2) named S. maltophilia flavin-containing monooxygenase (SMFMO), which catalyses the oxidation of thioethers and also the regioselective Baeyer-Villiger oxidation of the model substrate bicyclo[3.

Coccolithophores: functional biodiversity, enzymes and bioprospecting.

Emiliania huxleyi is a single celled, marine phytoplankton with global distribution. As a key species for global biogeochemical cycling, a variety of strains have been amassed in various culture collections. Using a library consisting of 52 strains of E.

The psychological and psychosocial impact of the Pakistan Kashmir earthquake after 8 months: a preliminary evaluation by PACTT.

Natural disasters are complex events that challenge the coping abilities of individuals and communities (Alexander, 2005). They are characterised by substantial loss, physical injury and economic hardship, as well as by extensive internal displacement and damage to the infrastructure, as exemplified by the Pakistan Kashmir earthquake of 8 October 2005. Measuring 7.

Psychological trauma in a relief worker--a case report from earthquake-struck areas of north Pakistan.

Vicarious traumatization is now a well-known entity and may have negative influences on those that are involved in rescue efforts in any disaster or traumatic events. Healthcare workers work with trauma survivors and witness an immense array of gruesome and ghastly images. This work has the potential to cause those engaged in rescue efforts to become affected subconsciously.

The structure of chorismate synthase reveals a novel flavin binding site fundamental to a unique chemical reaction.

The crystal structure of chorismate synthase (CS) from Streptococcus pneumoniae has been solved to 2.0 A resolution in the presence of flavin mononucleotide (FMN) and the substrate 5-enolpyruvyl-3-shikimate phosphate (EPSP). CS catalyses the final step of the shikimate pathway and is a potential therapeutic target for the rational design of novel antibacterials, antifungals, antiprotozoals, and herbicides.

Novel targets for antibiotic drug design.

The urgent need for new antibiotics has led to an explosion in the number and diversity of antibiotic drug targets under investigation. The majority of such targets are enzymes required for essential cellular functions. Often, such novel targets are completely unexploited for antibiotic therapy and therefore have the advantage of avoiding current resistance mechanisms.