Publications by authors named "Sohail A Shahzad"
Article Synopsis
- Excessive fluoride use and chloroform exposure pose health risks, prompting the need for effective sensors to monitor these substances.
- Researchers investigated etoricoxib (ECX) as a safe and available sensor, revealing its strong fluorescence response for detecting fluoride and chloroform at very low levels.
- Advanced analyses, including UV-Vis and DFT studies, confirmed that ECX operates through non-covalent interactions, making it a promising tool for real-time detection of fluoride and moisture in various samples.
A Multimode fluorescent sensor for sequential detection of Cu and cysteine as well as pH sensor with real sample Applications: Extensive experimental and DFT studies.
Spectrochim Acta A Mol Biomol Spectrosc
February 2025
Article Synopsis
- A new sensor called PDN has been developed that can selectively detect copper (Cu) and L-cysteine using a fluorescence On-Off-On approach and exhibits unique aggregation-induced emission enhancement (AIEE) properties.
- The sensor's effectiveness relates to its diazo functional group and specific molecular design, which contributes to its behavior, including a bathochromic shift due to J-aggregate formation.
- PDN shows impressive sensitivity with detection limits of 0.113 nM for Cu and 84 nM for L-cysteine and has been tested successfully in real-world samples, including strong acids and bases, and confirmed through various analytical methods and computational calculations.
Article Synopsis
- - A series of novel norfloxacin analogs were synthesized and characterized using NMR and mass spectrometry to assess their biological activity.
- - One specific compound showed impressive potency against HeLa cancer cells, achieving 100% inhibition of cell viability at an IC of 3.1 ± 0.2 μM, while all compounds displayed moderate to excellent antioxidant properties.
- - The compounds adhered to Lipinski's rule of drug-likeness and demonstrated favorable absorption characteristics, indicating potential for future development as anticancer drugs.
Article Synopsis
- High concentrations of deferasirox (DFX) can lead to serious health issues in the liver, stomach, and kidneys, making it crucial to develop an effective detection method to safeguard public health.
- Researchers created a thiourea-based probe called MPT that detects DFX by utilizing fluorescence quenching, a process where light emission decreases due to interaction with DFX.
- Various analytical techniques, including H-NMR titration, dynamic light scattering, and electron microscopy, were used to confirm the interaction between MPT and DFX, demonstrating its ability to detect DFX in real samples like human plasma and artificial urine.
Article Synopsis
- Metal-organic frameworks (MOFs) are explored as innovative alternatives to conventional antibiotic treatments for infections, utilizing chitosan membranes loaded with copper-MOF.
- The study confirms the safety and compatibility of the materials through various tests, revealing effective antibacterial properties of the higher concentration copper–MOF dressing against multiple bacterial strains, including MRSA.
- In a rat model for infected wounds, the copper-MOF dressings significantly enhanced healing, showing greater tissue regeneration and reduced inflammation compared to standard treatments.
A combined experimental and theoretical approach for doxycycline sensing using simple fluorescent probe with distinct fluorescence change in wide range of interferences.
Spectrochim Acta A Mol Biomol Spectrosc
June 2024
Article Synopsis
- Overuse of doxycycline can negatively impact human health, the environment, and food quality, highlighting the need for effective detection methods.
- Researchers developed a fluorescent probe named BBP that utilizes fluorescence spectroscopy to selectively and sensitively detect doxycycline via Forster resonance energy transfer (FRET).
- The study confirmed the sensing mechanism through various methods including density functional theory (DFT) and practical applications of BBP for detecting doxycycline in fish, wastewater, and blood samples.
Article Synopsis
- - A new isophthalamide-based sensor called IPA has been developed that exhibits strong aggregation-induced emission (AIE) properties, emitting light at 438 nm, making it sensitive and selective in detecting nitrobenzene (NB) in various states (solid, solution, vapor).
- - The interaction between IPA and nitrobenzene has been characterized through various spectroscopic methods, revealing significant fluorescence quenching and allowing NB detection down to levels of 60 nM, along with additional studies confirming the type of interaction.
- - Advanced computational methods, including DFT calculations and NBO analysis, were employed to explore the thermodynamic stability and charge transfer interactions of IPA with nitrobenzene, demonstrating its potential for practical
Article Synopsis
- The research focused on creating new chloroquine analogues to test their effectiveness against microbes and cancer cells.
- The synthesized analogues were found to have significant antimicrobial properties, with one analogue showing a 30.3 mm zone of inhibition against a specific microbe.
- Additionally, one analogue demonstrated strong cytotoxic effects on HeLa cells, achieving 100% cell inhibition at a low concentration, outperforming the original chloroquine.
Article Synopsis
- Researchers synthesized new amodiaquine analogues to investigate their potential therapeutic benefits.
- Characterization was done using advanced techniques like H NMR, C NMR, and FT-IR, confirming their structure and effectiveness.
- The compounds showed strong antibacterial and antifungal properties, with AS1 exhibiting significant cancer cell toxicity, indicating their possible use in medical treatments.
An extensive experimental and DFT studies on highly selective detection of nitrobenzene through deferasirox based new fluorescent sensor.
Spectrochim Acta A Mol Biomol Spectrosc
February 2024
Article Synopsis
- - A new sensor called TAD, based on deferasirox and a substituted triazole amine, has been developed to detect nitrobenzene specifically in various samples.
- - TAD shows a strong selective response to nitrobenzene compared to other nitroaromatic compounds, with the detection mechanism involving a photoinduced electron transfer (PET) process supported by various analytical methods like UV-visible spectroscopy and DFT analysis.
- - The research also included in-depth analyses using non-covalent interaction studies and energy decomposition to understand how TAD interacts with nitrobenzene, and the sensor was successfully tested in real-world applications.
Article Synopsis
- The study examines the role of β-glucosidase in hyperglycemia and non-alcoholic fatty liver disease, leading to liver cancer (HepG2).
- Researchers synthesized phthalimide and phthalamic acid analogs, which showed potential as inhibitors of β-glucosidase, utilizing in silico and in vitro techniques for analysis.
- Results indicated that some synthesized compounds had superior inhibitory effects compared to standard treatments, and further testing demonstrated their cytotoxicity against HepG2 cancer cell lines.
Triazine based fluorescent sensor for sequential detection of Hg and L-Cysteine in real samples and application in logic Gate: A combination of Extensive experimental and theoretical analysis.
Spectrochim Acta A Mol Biomol Spectrosc
November 2023
Article Synopsis
- A new fluorescent sensor called TBT was developed to detect mercury (Hg) ions and L-cysteine (Cys) sequentially, leveraging its sulfur moiety and molecular structure for effective sensing.
- When Hg is added to TBT, its fluorescence intensity increases due to the blocking of charge transfer and enhanced fluorescence, allowing for selective detection of both Hg and Cys through different mechanisms.
- Advanced studies, including NMR and DFT analyses, confirmed the interactions between TBT, Hg, and Cys, while the sensor demonstrated a low detection limit of 61.9 nM for Hg, and was utilized for both qualitative and quantitative analysis in real samples, integrating a logic
New fluorescent probe for sensing of mefenamic acid in aqueous medium: An integrated experimental and theoretical analysis.
Sanwa Rafique Abdullah Yahya Abdullah Alzahrani Khadija Hasher Irshad Asad Muhammad Khan Sohail Anjum Shahzad
Spectrochim Acta A Mol Biomol Spectrosc
November 2023
Article Synopsis
- * A new sensor, ECO, was designed to detect MFA using fluorescence spectroscopy, displaying a "turn-off" response based on a mechanism involving photoinduced electron transfer and hydrogen bonding.
- * The sensor's effectiveness was tested with human blood and urine samples, and its detection mechanism was supported by various analytical methods, including H NMR titration and density functional theory calculations.
Theoretical and experimental studies on mechanochromic triphenylamine based fluorescent "ON-OFF-ON" sensor for sequential detection of Fe and deferasirox.
Abdullah Yahya Abdullah Alzahrani Khanzadi Omama Khan Sanwa Rafique Hasher Irshad Khadija Sohail Anjum Shahzad
Spectrochim Acta A Mol Biomol Spectrosc
September 2023
Article Synopsis
- - A novel TPA-based sensor called TTU was designed to show mechanochromic and AIEE properties, allowing it to detect iron (Fe) in water with high specificity due to its ability to form a complex with paramagnetic Fe.
- - When deferasirox (DFX) is introduced to the TTU-Fe complex, the fluorescence of TTU is restored, indicating that DFX displaces Fe, which indicates a potential method for detecting DFX.
- - The mechanisms behind the interactions of TTU with Fe and DFX were validated through H NMR titration and DFT calculations, and TTU proved effective in real water sample tests, also contributing to the development of a logic gate for sequential
Article Synopsis
- - The research focuses on designing synthetic fluorescent probes (SH1 and SH2) to specifically detect harmful hypochlorite ions (ClO), which are a type of reactive oxygen species (ROS) that can negatively affect living organisms.
- - The probes utilize a thiourea moiety that reacts selectively with ClO, enabling rapid and sensitive detection at nanoscale concentrations, and have been validated through various scientific methods.
- - Additionally, the probes showed low toxicity to live cells, can effectively monitor ClO in blood serum and water samples, and portable fluorescent films were created for easy detection, suggesting potential for future clinical applications.
Article Synopsis
- Hypochlorite ions (ClO) are a type of reactive oxygen species involved in various biological processes, but excessive levels can lead to health issues like cancer.
- Two new fluorescent compounds (probe 1 with catechol and probe 2 with 2-naphthyl) were developed to detect ClO in food and industrial applications, with probe 1 showing better performance than probe 2.
- Probe 1 is particularly notable for its quick response time and lower detection limit, and it was effectively used to track ClO levels in industrial samples and demonstrated versatility in monitoring ClO in sprouting potatoes.
AIEE active J-aggregates of naphthalimide based fluorescent probe for detection of Nitrobenzene: Combined experimental and theoretical approaches for Non-covalent interaction analysis.
Spectrochim Acta A Mol Biomol Spectrosc
April 2023
Article Synopsis
- A new fluorescent probe called NS was developed using a simple chemical reaction, showcasing impressive aggregation-induced emission enhancement (AIEE) in water due to the formation of J-aggregates.
- This probe can selectively and sensitively detect nitrobenzene (NB) by observing changes in fluorescence, with the size of NB allowing easy access into the probe's structure.
- The study incorporated various analytical methods, including spectroscopy and theoretical calculations, to understand the interactions between the probe and NB, demonstrating the probe's effectiveness in both solid and vapor-phase detection of NB, even in low concentrations within real samples.
Article Synopsis
- * The results show that the presence of DOM significantly enhances mercury binding due to increased binding sites created by the composite of bentonite and DOM, despite a reduction in binding efficiency at higher DOM concentrations.
- * Advanced techniques like XPS and FTIR confirm that modifications in the clay’s surface properties, particularly oxygen functionalities, play a crucial role in the enhanced uptake of mercury, suggesting implications for managing mercury in environments rich in organic matter.
AIEE active sensors for fluorescence enhancement based detection of Ni in living cells: Mechanofluorochromic and photochromic properties with reversible sensing of acid and base.
Mohammed A Assiri Hafiz Muhammad Junaid Muhammad Tahir Waseem Asad Hamad Sajid Hussain Shah Sohail Anjum Shahzad
Anal Chim Acta
November 2022
Article Synopsis
- Two advanced sensors, QI 1 and QI 2, were created to detect nickel ions (Ni) using various methods, including color change and fluorescence, showcasing unique properties such as mechanofluorochromism and photochromism.
- The sensors achieved low detection limits of 12 nM and 17 nM for Ni using a mechanism called chelation enhanced fluorescence (CHEF), which suppresses intramolecular proton transfer.
- The effectiveness of these sensors was demonstrated in live cells and industrial waste, and they also contributed to the development of a logic gate system for electronic analysis involving nickel and EDTA.
Design, Synthesis, and Biological Evaluation of Novel Dihydropyridine and Pyridine Analogs as Potent Human Tissue Nonspecific Alkaline Phosphatase Inhibitors with Anticancer Activity: ROS and DNA Damage-Induced Apoptosis.
Nazeer Ahmad Khan Faisal Rashid Muhammad Siraj Khan Jadoon Saquib Jalil Zulfiqar Ali Khan Sohail Anjum Shahzad
Molecules
September 2022
Small molecules with nitrogen-containing scaffolds have gained much attention due to their biological importance in the development of new anticancer agents. The present paper reports the synthesis of a library of new dihydropyridine and pyridine analogs with diverse pharmacophores. All compounds were tested against the human tissue nonspecific alkaline phosphatase (-TNAP) enzyme.
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- * This research focuses on synthesizing isoxazole derivatives using a specific method involving sonication and a catalyst, and the products were thoroughly characterized.
- * Testing showed that two of the synthesized compounds, AC2 and AC3, are strong inhibitors of the carbonic anhydrase enzyme, with AC2 demonstrating the most impressive activity, suggesting they could be valuable for developing new enzyme inhibitors.
The search for novel heterocyclic compounds with a natural product skeleton as potent enzyme inhibitors against clinical hits is our prime concern in this study. Here, a simple and facile two-step strategy has been designed to synthesize a series of novel coumarin-based dihydropyranochromenes (-) in a basic moiety. The synthesized compounds were thus characterized through spectroscopic techniques and screened for inhibition potency against the cytosolic hCA II isoform and β-glucuronidase.
View Article and Find Full Text PDF(1) Background: belongs to a highly reputed family of medicinal plants, with plant extract being used as medicine in indigenous system. However, limited data is available regarding the exploitation of the medicinal potential of isolated pure compounds from this family; (2) Methods: A whole plant extract was partitioned into fractions and on the basis of biological activity, an ethyl acetate fraction was selected for isolation of pure compounds. Isolated compounds were characterized using different spectroscopic techniques.
View Article and Find Full Text PDFApart from environmental implications, the extreme toxicity of cyanide can lead to sudden human death upon prolonged exposure to it. Hence, rapid and low-level on-site detection of cyanide has earned paramount significance in the present era. Therefore, an AIEE active and piezofluorochromic Schiff base (probe 2) was synthesized which exhibited highly selective fluorescence enhancement based nanoscale (LOD; 6.
View Article and Find Full Text PDFRational modification of molecular structure by incorporating electron donating groups can play a potential role for designing aggregation induced emission (AIE) active fluorescent probes. Based on this principle, fluorescent probes (1a-c) were synthesized, and they displayed excellent aggregation induced emission (AIE) behavior in a HO/DMF (4 : 1, /) mixture due to restrictions in intramolecular charge transfer (ICT). As a comparison, probe 1d was synthesized by installing an electron withdrawing (-NO) group that surprisingly quenched the aggregation behaviour.
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