Fucosylated Chondroitin Sulfates with Rare Disaccharide Branches from the Sea Cucumbers and : Structures and Influence on Hematopoiesis.

Two fucosylated chondroitin sulfates were isolated from the sea cucumbers and using a conventional extraction procedure in the presence of papain, followed by anion-exchange chromatography on DEAE-Sephacel. Their composition was characterized in terms of quantitative monosaccharide and sulfate content, and structures were mainly elucidated using 1D- and 2D-NMR spectroscopy. As revealed by the data of the NMR spectra, both polysaccharides along with the usual fucosyl branches contained rare disaccharide branches α-D-GalNAc46-(1→2)-α-L-Fuc34 → attached to -3 of the GlcA of the backbone ( = H or SO).

Glycosaminoglycans from the Starfish : Structures and Influence on Hematopoiesis.

Crude anionic polysaccharides extracted from the Pacific starfish were purified by anion-exchange chromatography. The main fraction having MW 14.5 kDa and dispersity 1.

Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers and : Structures and Anticoagulant Activity.

Fucosylated chondroitin sulfates (FCSs) and , as well as fucan sulfates (FSs) and were isolated from the sea cucumbers and , respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural characterization of polysaccharides was performed in terms of monosaccharide and sulfate content, as well as using a series of non-destructive NMR spectroscopic methods.