Effects of sphingosine 1-phosphate on calcium signaling, proliferation and S1P2 receptor expression in PC Cl3 rat thyroid cells.

Sphingosine 1-phosphate (S1P) regulates diverse biological processes, including mitosis, by binding to the S1P family of G-protein coupled receptors. The aim of the study was to determine the pattern of S1P receptor expression and to investigate the effects of S1P on intracellular calcium levels and proliferation in the rat thyroid cell line PC Cl(3). S1P(2) and S1P(3) mRNA and proteins were detected in PC Cl(3) cells, as well as in FRTL-5 rat thyroid cells.