A robust and simple non-enzymatic electrochemical sensor based on carbon dots-metal oxide composite for the detection of metronidazole traces in food products.

A facile and simple electrochemical composite sensor, CDs-Ag@CuO-GA, prepared from carbon dots stabilized silver nanoparticles and copper oxide, was used as an electrocatalyst and signal amplifier for the non-enzymatic detection of antibiotic traces in food products. The prepared composite demonstrated excellent stability, sensitivity, and cost-effectiveness. The sensor was constructed by modifying a glassy carbon electrode (GCE) with CDs-Ag@CuO-GA, and the electroanalytical response was determined for the precise determination of metronidazole (MTZ) drug traces in milk.

Biochemical properties of novel Carbon nanodot-stabilized silver nanoparticles enriched calcium hydroxide endodontic sealer.

Calcium Hydroxide-based endodontic sealer loaded with antimicrobial agents have been commonly employed in conventional root canal treatment. These sealers are not effective against E. faecalis due to the persistent nature of this bacterium and its ability to evade the antibacterial action of calcium hydroxide.

Analysis of the action mechanisms and targets of herbal anticonvulsants highlights opportunities for therapeutic engagement with refractory epilepsy.

Epilepsy is a neurological disorder characterized by spontaneous and recurring seizures. It poses significant therapeutic challenges due to diverse etiology, pathobiology, and pharmacotherapy-resistant variants. The anticonvulsive effects of herbal leads with biocompatibility and toxicity considerations have attracted much interest, inspiring mechanistic analysis with the view of their use for engagement of new targets and combination with antiseizure pharmacotherapies.

Eco-friendly synthesis of mesoporous bioactive glass ceramics and functionalization for drug delivery and hard tissue engineering applications.

Hard tissue regenerative mesoporous bioactive glass (MBG) has traditionally been synthesized using costly and toxic alkoxysilane agents and harsh conditions. In this study, MBG was synthesized using the cheaper reagent SiOby using a co-precipitation approach. The surface properties of MBG ceramic were tailored by functionalizing with amino and carboxylic groups, aiming to develop an efficient drug delivery system for treating bone infections occurring during or after reconstruction surgeries.

Photoswitchable nonlinear optical properties of azobenzene-based supramolecular complexes: insights from density functional theory.

Density functional theory (DFT) calculations were performed for a series of supramolecular assemblies containing azobenzene (Azo-X where X = I, Br and H) and alkoxystilbazole subunits to evaluate their electronic, linear and nonlinear optical properties. These assemblies are derivatives of azobenzene, obtained by the substitution of electron-withdrawing and electron-donating groups onto the molecular skeleton. The interaction energies () of all the designed supramolecular complexes (IA-IF, IIA-IIF and IIIA-IIIF) range from -1.

In Vitro Analysis of Cytotoxic Activities of Targeting / Genes in Breast Cancer Cells.

Breast cancer (BC) is known to be the most common malignancy among women throughout the world. Plant-derived natural products have been recognized as a great source of anticancer drugs. In this study, the efficacy and anticancer potential of the methanolic extract of leaves using human breast cancer cells targeting / signaling was evaluated.

Therapeutic Potential of Selenium Nanoparticles on Letrozole-Induced Polycystic Ovarian Syndrome in Female Wistar Rats.

Polycystic ovarian syndrome (PCOS) is considered the most frequent gynecological endocrine disorder that causes anovulatory infertility. The current study aimed to investigate the potential significance of selenium nanoparticles (SeNPs), an IL-1 inhibitor, in the treatment of letrozole-induced PCOS in rats that satisfied the metabolic and endocrine parameters found in PCOS patients. Letrozole (2 ppm, per orally, p.

Efficacy of Phytochemicals Derived from Roots of as Antioxidant, Antiulcer, Diuretic, Skin Brightening and Hemolytic Agents-A Comprehensive Biochemical and In Silico Study.

Roots of are a rich source of phytochemicals with high antioxidant potential and thus may possess health benefits. This study used the LC-MS technique to identify phytoconstituents in roots extract/fractions. Results revealed that -butanol fraction and ethanolic extract contained total phenolic and flavonoid contents with values of 155.

Mixed superalkalis are a better choice than pure superalkalis for BN nanocages to design high-performance nonlinear optical materials.

Mixed superalkali clusters are a source of excess electrons, as their vertical ionization energies (2.81-3.36 eV) are much lower than those of alkali metals (even cesium (∼3.

Phytochemical Profiling, Antioxidant, Anti-Inflammatory, Thrombolytic, Hemolytic Activity In Vitro and In Silico Potential of .

The use of complementary herbal medicines has recently increased in an attempt to find effective alternative therapies that reduce the adverse effects of chemical drugs. is a rich source of phytochemicals with high antioxidant activity, and thus may possess health benefits. This study used the latest developments in GC-MS coupling with molecular docking techniques to identify and quantify the phytoconstituents in tissue extracts.

Neuroprotective Effect of Against PTZ-Induced Mice Model of Epilepsy by Attenuated Expression of p-NFκB and TNF-α.

Epilepsy is a chronic neurological disorder characterized by recurrent unprovoked Currently available antiepileptic drugs have severe side effects and do not offer complete cure. Herbal remedies have been used for centuries to treat many neurodegenerative disorders. L.

Variations in kinase gladiators and risk of differentiated thyroid carcinoma.

Thyroid carcinoma (TC) accounts for ~2.1% of newly diagnosed cancer cases. Mutations in , , and are primary participants in the development and progression of various types of malignancy, including differentiated TC (DTC).

N-Acetyl Cysteine, Selenium, and Ascorbic Acid Rescue Diabetic Cardiac Hypertrophy via Mitochondrial-Associated Redox Regulators.

Metabolic disorders often lead to cardiac complications. Metabolic deregulations during diabetic conditions are linked to mitochondrial dysfunctions, which are the key contributing factors in cardiac hypertrophy. However, the underlying mechanisms involved in diabetes-induced cardiac hypertrophy are poorly understood.

Asporychalasin, a bioactive cytochalasan with an unprecedented 6/6/11 skeleton from the Red Sea sediment Aspergillus oryzae.

The cytochalasan asporychalasin (1) was obtained from the marine fungus Aspergillus oryzae, isolated from the Red Sea sediments collected off Jeddah, Saudi Arabia. The chemical structure of 1 was elucidated by extensive spectroscopic analysis and quantum-mechanical calculations of C NMR resonances and ECD to possess an unprecedented 6/6/11-fused tricyclic skeleton, including an isoquinolindione ring in place of the typical isoindolone. Asporychalasin exhibited moderate antiproliferative activity against three human cancer cell lines, lung carcinoma (A549), liver carcinoma (HepG2), and breast carcinoma (MCF7), and no toxicity on zebrafish embryos.

Discrepancies of RET gene and risk of differentiated thyroid carcinoma.

Background: Somatic variations in rearranged during transfection (RET) proto-oncogene acts to influence Thyroid cancer (TC) in a low penetrance manner, but their effects tend to vary between different populations.

Objective: This case-control study was aimed to evaluate effect of RET G691S, S904S and L769L single nucleotide polymorphisms (SNPs) on the risk for differentiated thyroid carcinoma (DTC).

Methods: A total of 180 patients and 220 controls were genotyped by Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP).

Antiproliferative Illudalane Sesquiterpenes from the Marine Sediment Ascomycete .

The new asperorlactone (), along with the known illudalane sesquiterpene echinolactone D (), two known pyrones, 4-(hydroxymethyl)-5-hydroxy-2-pyran-2-one () and its acetate , and 4-hydroxybenzaldehyde (), were isolated from a culture of , collected from Red Sea marine sediments. The structure of asperorlactone () was elucidated by HR-ESIMS, 1D, and 2D NMR, and a comparison between experimental and DFT calculated electronic circular dichroism (ECD) spectra. This is the first report of illudalane sesquiterpenoids from fungi and, more in general, from ascomycetes.

Phytochemical Analysis of Leaves: Identification of Garcinamines F-H and Their Antiproliferative Activities.

is a medicinal plant used in the Arab region for intestinal diseases, lung and liver diseases, digestive problems, and as an antidiabetic agent. Repeated chromatographic purifications of leaves led to the isolation of three undescribed guaiane sesquiterpene derivatives, named garcinamines F-H, characterized by the presence of an amino acid unit, along with five known sesquiterpene lactones (garcinamines B-E and 9β-hydroxyparthenolide). The structures of the new compounds were established using spectroscopic (1D and 2D NMR) and spectrometric methods (ESIMS).

Mitotic nuclei analysis in breast cancer histopathology images using deep ensemble classifier.

Mitotic nuclei estimation in breast tumour samples has a prognostic significance in analysing tumour aggressiveness and grading system. The automated assessment of mitotic nuclei is challenging because of their high similarity with non-mitotic nuclei and heteromorphic appearance. In this work, we have proposed a new Deep Convolutional Neural Network (CNN) based Heterogeneous Ensemble technique "DHE-Mit-Classifier" for analysis of mitotic nuclei in breast histopathology images.

Oral versus Vaginal Micronized Progesterone for the Treatment of Threatened Miscarriage.

Objectives: This study was planned with an aim to find out the effectiveness of oral versus vaginal micronized progesterone for the treatment of threatened miscarriage.

Methods: This randomized controlled trial was conducted at The Department of Obstetrics and Gynaecology, Nishtar Hospital Multan, from August 2019 to January 2020. A total of 136 pregnant women, aged 18 to 45 years having vaginal bleeding were included and divided into two groups (68 women in each group).

Radioprotective Potential of Nutraceuticals and their Underlying Mechanism of Action.

Radiations are an efficient treatment modality in cancer therapy. Besides the treatment effects of radiations, the ionizing radiations interact with biological systems and generate reactive oxygen species that interfere with the normal cellular process. Previous investigations have been conducted only on few synthetic radioprotectors, mainly owing to some limiting effects.

VE1 immunohistochemistry is an adjunct tool for detection of BRAF mutation: Validation in thyroid cancer patients.

Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy among other endocrine tumors, and BRAF is a frequent genetic mutation occurring in the disease. Although different molecular techniques, most importantly sequencing has been widely recognized as a gold standard but molecular diagnosis remains an expensive, laborious, and time-intensive process. Recently, immunohistochemistry (IHC) with anti-BRAF V600E (VE1) antibody has increased practical utility and implemented clinically for the detection of BRAF mutation.