Effectiveness of Gamma Rays in Attenuation of Toxoplasma gondii Pathogenicity and Eliciting Immune Response in Mice.

Gamma irradiation was applied to the tachyzoites Toxoplasma gondii virulent strain at doses of 0.25, 0.5, 1, 1.

Attenuation of pathogenesis of Eimeria stiedae sporulated oocysts using Egyptian alginate propolis nanoparticles.

Background: Coccidiosis is a costly and widespread infectious disease that affects mammals and causes huge losses for the global rabbit meat industry. This study evaluated the potency of Egyptian alginate propolis nanoparticles (NPs) in attenuating the infectivity of Eimeria stiedae sporulated oocysts. The gelification method was used to prepare alginate propolis NPs, which were then characterized using a transmission electron microscope and zeta potential analysis.

Evaluation of a vaccine candidate isolated from oocyst in mice.

Background And Aim: Cryptosporidiosis is a leading cause of diarrheal disease worldwide and is an animal and public health burden. This study aimed to evaluate the protective potential of affinity-purified oocyst antigen as a vaccine candidate according to fecal oocyst shedding, humoral and cellular immune responses, histopathological changes, and the number of parasite developmental stages in ileal and hepatic tissues.

Materials And Methods: We isolated oocysts from naturally infected buffalo calves and identified them molecularly as isolates (GenBank: ON730707 and ON730708) by targeting the oocyst wall protein gene.

Assessment of larval and adult somatic antigens in sero-diagnosis of haemonchosis in naturally infected sheep and goats.

The current work was carried out to evaluate the potency of larval and adult somatic () antigens in detection of haemonchosis among sheep and goats using ELISA. Two hundred and forty-three fecal and blood samples were randomly collected from small ruminants (107 sheep and 136 goats) in Beni-Suef Governorate, Egypt, during the period from June to August 2018. The fecal analysis exhibited that 26.

Immunomodulatory effects of leaves aqueous extract in sheep naturally co-infected with and .

The current study was designed to evaluate the in vivo fasciolicidal activity of () leaf aqueous extract oral administration as well as its antibacterial activity against () sheep naturally co-infected with fascioliasis and . Sheep naturally infected with fascioliasis were divided into 3 groups, heavily infected treated group, lightly infected treated group and mixed infection control (non-treated) group. Treatment groups were orally administered leaves aqueous extract at a dose of 150 mg/kg every 48 h for 21 days.

Cellular immune response and scanning electron microscopy in the evaluation of Moringa leaves aqueous extract effect on in buffalo intestinal tissue explants.

is an apicomplexan parasite of human and animals and is considered as an important co-factor in neonatal diarrhea. In this study, an explant culture was used as an in vitro model of buffalo intestine to evaluate the effect of Moringa leaves extract on () oocysts using light and scanning electron microscopy and measuring IFN-γ, IL-12 and IL-14 in the culture supernatants. oocysts were collected from naturally-infected calf feces, isolated, excysted and then co-inoculated with ileal tissue explants culture medium.

cuticle glycoproteins in the diagnosis of calves' toxocariasis.

Aim: The current study was designed to isolate and characterize glycoprotein antigens and then to evaluate its potency in accurate diagnosis of toxocariasis.

Materials And Methods: glycoprotein fractions were isolated using Con-A affinity chromatography. The fractions characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and immunoblot assay.

Comparative ovicidal activity of leaf extracts on eggs.

Background: Fasciolosis is an important zoonotic disease affecting the productive performance of farm animals in Egypt.

Aim: The aim of the present study was comparing the ovicidal effect of different extracts as an alcoholic (Methanolic and Ethanolic) and aqueous leaf extracts on non-embryonated and developed eggs.

Materials And Methods: Tested concentrations of extracts ranged from 12.

Botanical study of Meryta denhamii Seem. and its antihelminthic activity against Fasciola gigantica.

The anthelmintic activity of the ethanolic extracts of stems and leaves of Meryta denhamii Seem. against adult liver flukes "Fasciola gigantica" was studied in vitro. Although leaves extract was inactive, stems extract exhibited anthelmintic activity and recorded LC50 and LC90 values, 16 and 26 gm/l respectively.

Molluscicidal saponins from leaves of Hedera canariensis.

Activity-guided fractionation of the methanol extract of Hedera canariensis (var. Gloire de Maringo) Wild leaves afforded five saponins 1-5. Chemical and spectral methods (MS, 1HNMR, 13CNMR) showed that they are glycosides of hederagenin and oleanolic acid.