Publications by authors named "So Yun Han"
Obstructive Sleep Apnea-induced Endothelial Dysfunction Is Mediated by miR-210.
Am J Respir Crit Care Med
February 2023
Obstructive sleep apnea (OSA)-induced endothelial cell (EC) dysfunction contributes to OSA-related cardiovascular sequelae. The mechanistic basis of endothelial impairment by OSA is unclear. The goals of this study were to identify the mechanism of OSA-induced EC dysfunction and explore the potential therapies for OSA-accelerated cardiovascular disease.
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- The study addresses the challenge of isolating cells based on their secreted proteins by using a high-throughput method that integrates heterofunctional particles and fluorescence-activated cell sorting (FACS).
- The authors successfully isolate antibody-secreting cells (ASCs) that are specific for the H1 protein of the influenza virus from B cells by capturing their secreted immunoglobulins with these particles.
- The method shows high sensitivity (96%) and specificity (98%) for targeted hybridoma cells, ultimately demonstrating a significant enrichment of H1-specific ASCs through the use of optimized particle designs.
Bead reagents are used in a large number of assays in bioscience and biotechnology to collect and purify antibodies by immobilization. Bead-based immunoassays offer high-throughput analysis of multiple antibodies in a single sample. Although a variety of antibody-binding moieties on the collection beads have been studied, the physical and material properties of collection beads have not been optimized to isolate specific antibodies over a broad range of concentrations from complex environments containing cells.
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