Studies on the Efficiency of Iron Release from Fe(III)-EDTA and Fe(III)-Cit and the Suitability of These Compounds for Tetracycline Degradation.

Iron ions can be used to degrade tetracycline dispersed in nature. Studies of absorption and fluorescence spectra and quantum chemistry calculations showed that iron is more readily released from Fe(III)-citrate than from Fe(III)-EDTA, so Fe(III)-citrate (Fe(III)-Cit) is more suitable for tetracycline (TC) degradation. At 30 °C, a severe degradation of TC by Fe(III)-Cit occurred as early as after 3 days of incubation in the light, and after 5 days in the dark.

The effect of polyunsaturated fatty acids on cardiovascular risk factors and urinary mercury in workers employed at electrolytic production of chlorine.

Objectives: While inorganic mercury is being gradually withdrawn from industry, environmental exposure to mercury is recognized as one of the greatest present toxicological problems. The aim of this study was to evaluate the effect of polyunsaturated fatty acids (PUFAs) supplementation on selected cardiovascular risk factors and the urinary mercury (Hg-U) concentration in workers occupationally exposed to mercury vapor.

Material And Methods: Overall, 38 workers of an electrolyzer hall (Hg-U: 46.

Spectroscopic Evidence for Photooxidation of Tocopherols in n-Hexane.

This paper presents the results of an investigation into the photooxidation of tocopherols (Tocs) dissolved in argonated and non-argonated n-hexane. During irradiation, steady-state absorption and fluorescence spectra as well as lifetimes were measured. In all experiments, the photoreactions were of the first order type.

Excited-State Proton Transfer in 8-Azapurines I: A Kinetic Analysis of 8-Azaxanthine Fluorescence.

A super-continuum white laser with a half-pulse width of ~75 ps was used to observe the kinetics of a postulated excited-state proton transfer in 8-azaxanthine and its 8-methyl derivative. Both compounds exhibited dual emissions in weakly acidified alcoholic media, but only one band was present in aqueous solutions, exhibiting an abnormal Stokes shift (>12,000 cm). It was shown that long-wavelength emissions were delayed relative to the excitation pulse within alcoholic media.

The relationship between PNP, GSTO-1, AS3MT and ADRB3 gene polymorphisms and urinary arsenic concentration among copper smelter and refinery employers.

Introduction: The aim of this study was to assess the relationship between polymorphisms of genes encoding enzymes involved in arsenic metabolism and urinary arsenic concentration in people occupationally exposed to arsenic.

Materials And Methods: The data from 113 employers directly exposed to lead, cadmium, and arsenic in copper smelter in Legnica and Glogow were collected. Urinary arsenic concentration was measured.

The Relationship Between Selected CNR1, MC4R, LEP, FTO and VDR Gene Polymorphisms and Several Basic Toxicological Parameters Among Persons Occupationally Exposed to Arsenic, Cadmium and Lead.

The purpose of this work was to assess the influence of selected CNR1, MC4R, LEP, FTO and VDR FOKI gene polymorphisms on blood and urine concentration markers of lead, cadmium and arsenic in a population directly exposed to these metals. Eighty-five people exposed to lead, arsenic and cadmium were qualified to take part in the study. Standard urine samples and 25mL of venous blood from each worker were collected to assay basic laboratory and toxicological markers as well as selected single nucleotide polymorphisms (SNPs) within CNR1-cannabinoid receptor 1 gene (), MC4R-melanocortin 4 receptor gene (), LEP-leptin promoter gene (), FTO-alpha-ketoglutarate-dependent dioxygenase gene () and VDR-vitamin D receptor () genes.

Spectroscopic demonstration of sinapic acid methyl ester complexes with serum albumins.

The methyl ester of sinapic acid (MESA) is a molecule with confirmed antioxidant properties. It is important to establish whether it can be transported across humans and animals. Therefore, we investigated MESA interactions with serum albumins, namely, human serum albumin (HSA), bovine serum albumin (BSA), rabbit serum albumin (RSA), and sheep serum albumin (SSA).

Influence of light and Fe(III) ions on tetracycline degradation.

Tetracycline (TC) is an antibiotic produced on the largest scale in the world and used for the treatment of both humans and animals. Its removal from the circulation chain between the natural environment and animals is still a serious problem. Fe(III) ions can be used to break this chain.

A new approach to the old problem: Inner filter effect type I and II in fluorescence.

The fluorescence technique is very popular and has been used in many fields of research. It is simple in its assumptions but not very easy to use. One of the main problems is the inner filter effect (IF) I and II which takes place in the cuvette.

Spectroscopic Properties and Conformational Analysis of Methyl Ester of Sinapic Acid in Various Environments.

A methyl ester of sinapic acid (MESA) has recently attracted attention due to its antioxidant action. This article presents results of a study on the spectral and physicochemical properties of MESA, using quantum chemistry (QC), steady-state (absorption and fluorescence), and time-resolved fluorescence techniques (TCSPC). The pK of the phenol group in the ground state was determined (8.

Unsaturated fatty acids supplementation reduces blood lead level in rats.

Some dietary factors could inhibit lead toxicity. The aim of this study was to evaluate the effect of dietary compounds rich in unsaturated fatty acids (FA) on blood lead level, lipid metabolism, and vascular reactivity in rats. Serum metallothionein and organs' lead level were evaluated with the aim of assessing the possible mechanism of unsaturated FA impact on blood lead level.

The relationship between selected VDR, HFE and ALAD gene polymorphisms and several basic toxicological parameters among persons occupationally exposed to lead.

Unlabelled: The aim of this study was to find a relationship between polymorphisms of ALAD rs1805313, rs222808, rs1139488, VDR FokI and HFE C282Y and H63D and basic toxicological parameters (lead and ZnPP blood concentration) in people occupationally exposed to lead. We collected data of 101 workers (age 25-63 years) directly exposed to lead. The toxicological lab tests included blood lead, cadmium and ZnPP concentration measurement and arsenic urine concentration measurement.

Singlet oxygen autoxidation of vegetable oils: evidences for lack of synergy between β-carotene and tocopherols.

The synergy between β-carotene and tocopherols--antioxidants protecting oils from oxidation, was analyzed in a model system. The model used stripped borage and evening primrose oils. A chlorophyll extract, β-carotene and one of the tocopherols were added together or separately to the oils.

Steady-state and time-resolved fluorescence studies of stripped Borage oil.

In this study we explored the spectroscopic properties of Borage oil, particularly the use of fluorescence techniques to investigate the presence of conjugated fatty acids (CFAs). This research has important health and dietary applications. The absorption and fluorescence spectra of different CFAs and Borage oil in ethanol were measured.

[The influence of occupational exposure to arsenic and heavy metals on the activity of catepsins and their inhibitors in blood serum of copper smelters].

Background: Exposure to arsenic, lead, and cadmium poses the risk of cancer. Cathepsins (CP) and their natural inhibitors (CPI) in blood serum are markers of carcinogensis. The aim of this work was to verify the existence of relationships between the exposure to the airesaid elements and the activity of CP and CPL.

Influence of UV radiation and nitrosamines on the induction of mycotoxins synthesis by nontoxigenic moulds isolated from feed samples.

The effects of UV radiation and nitrosamines on the induction of mycotoxin biosynthesis by some nontoxigenic moulds isolated from feed samples collected from Egypt and Poland was investigated. Nontoxigenic strains of Aspergillus flavus P-63, A. niger EN-200 and A.

Spectroscopic manifestations of flavomononucleotide dimers in polyvinyl alcohol films.

Absorption and fluorescence spectra of flavomononucleotide (FMN) in polyvinyl alcohol films (PVA) over a very wide concentration range are investigated. The dimerization constant as well as the pure monomer and dimer spectra are calculated and the structural parameters of FMN dimer are established. Excitation wavelength and temperature dependencies of FMN/PVA fluorescence spectra for different FMN concentrations were carried out.

Environmental risk factors of cancer and their primary prevention.

The evaluation of the influence of different environmental carcinogenic factors requires interdisciplinary cooperation. Related studies include epidemiological surveys and air, water and soil, chemical, toxicological, and microbiological analyses, supplemented by experimental verification of suspected ecological pathogens and cofactors. A balance of carcinogens and protective agents in the external environment and in the human body is recommended for an ecologically oriented prevention.

Effect of dicumarol on the growth of some soil microorganisms.

The effect of dicumarol on growth of selected soil bacteria: Azotobacter chroococcum, Arthrobacter globiformis, A. citreus and Bacillus megaterium was studied. The following minimum concentrations were inhibitory in vitro: Arthrobacter citreus--20 mug/ml.

Selenium effect on the growth of carcinogenic fungi and cytotoxic action of aflatoxin B1 on lymphocyte culture and on embryonal development of Xenopus laevis.

The authors report the results of investigations on the protective effect of selenium against teratogenic and cytotoxic action of aflatoxin B1 and the inhibitory action of this element on the growth of fungi producing carcinogenic aflatoxins. Sodium selenate inhibited the growth of cultures of such fungi belonging to the class Fungi imperfecti as Aspergillus flavus, Aspergillus fumigatus, Penicillium meleagrinum and Penicillium rougolosum. Added to cell cultures of lymphocytes sodium selenate inhibited the cytotoxic action of aflatoxin B1 evidenced by inhibition of PHA-stimulated blastic transformation of these cells and reduction in the number of mitoses.