[Effect of lomefloxacin on the synthesis of antibodies to vaccine EV fraction I and hemagglutinins].

Multifactor analysis of the lomefloxacin effect on the primary immune response to T-independent bacterial antigen (vaccine EV fraction 1) and to T-dependent cellular antigen (SRBC) in mice was performed with the use of a wide range of the antibiotic concentrations and the dosage time. It was shown that lomefloxacin in the doses corresponding to the average therapeutic ones used for 3-9 days had no effect on the content of the IgG antibodies, was able to increase the level of the IgM antibodies and insignificantly lowered the concentration of the IgA antibodies.

[The effect of norfloxacin on the luminol-dependent chemiluminescence and adhesion of leukocytes].

The in vitro effect of norfloxacin on the luminol-dependent chemiluminescence and adherence of polymorphonuclear leukocytes of guinea pigs was studied with the use of a wide range of the drug concentrations from 0.1 to 100 micrograms/ml under two different conditions, i.e.

[Effect of ciprofloxacin on luminol-dependent chemiluminescence and adhesion of leukocytes].

The in vitro effect of ciprofloxacin on the luminol-dependent chemiluminescence and adherence of polymorphonuclear leukocytes of guinea pigs was studied with the use of a wide variety of the drug concentrations from 0.1 to 1000 micrograms/ml under two different conditions: during incubation of the cells in the presence of the drug different concentrations and after the cell washing to remove ciprofloxacin. In concentrations of 1 to 100 micrograms/ml ciprofloxacin lowered the chemiluminescence at the average by 20 per cent and in a concentration of 1000 micrograms/ml the drug completely inhibited it.

[Multifactor analysis of the action of norfloxacin on the humoral immune response].

Multifactor analysis of the norfloxacin action on the primary immune response to the bacterial antigen (EV vaccine fraction 1) and cellular antigen (sheep erythrocytes) in mice was carried out with using a wide variety of the doses and administration times. In the doses corresponding to the average therapeutic ones norfloxacin was shown to have no effect on the humoral immune response to the bacterial and cellular antigens. In the subtherapeutic doses norfloxacin increased it thus mainly stimulating the synthesis of the IgM antibodies.

[Multifactor analysis of ciprofloxacin action on the content of various classes of antibodies to EV vaccine fraction 1 and hemagglutinins].

The action of ciprofloxacin on the content of antibodies of the IgG, IgM and IgA classes in the blood serum of mice immunized by the EV vaccine fraction 1 as well as on the content of hemagglutinins to sheep erythrocytes was studied in a multifactor analysis with the use of a wide variety of the doses and administration times. In the doses corresponding to the average therapeutic ones ciprofloxacin increased the content of the antibody classes and especially that of IgG. When administered prior to the immunization the antibiotic stimulated the production of the hemagglutinins.

[Multifactor analysis of the effect of pefloxacin on the dynamics of antibody synthesis to EV vaccine fraction 1 and hemagglutinins].

Multifactor analysis of the effect of pefloxacin on the primary immune response to T-independent bacterial antigen (EV vaccine fraction 1) and to T-dependent cellular antigen (sheep erythrocytes) in mice was carried out with the use of a wide variety of the doses and administration times. It was shown that in the doses corresponding to the average therapeutic ones pefloxacin had no affect on the humoral immune response to the EV vaccine fraction 1 antigens while with the use of the subtherapeutic doses the effect was stimulating. When the doses were higher than the therapeutic ones pefloxacin retarded the antibody formation.

[Effect of pefloxacin on luminol-dependent chemiluminescence and leukocyte adhesion].

The in vitro action of pefloxacin on the luminole-dependent chemiluminescence and adherence of polymorphonuclear leukocytes of guinea pigs was studied within a wide range of the concentrations from 0.1 to 100 micrograms/ml. Pefloxacin did not influence the peak values of the chemiluminescence while in concentrations of 0.

[Immunomodulating action of peptidoglycan of microbial origin].

The action of a high molecular weight peptidoglycan produced by Agrobacter radiobacter sp. on the functional activity parameters in leukocytes and macrophages i. e.

[Effect of rifampicin on the synthesis of antibodies to fraction I of vaccine EV].

Multifactorial analysis was used to study the influence of rifampicin on the dynamics of synthesis of antibodies belonging to IgM and IgG classes in mice immunized by fraction I of the vaccinal strain EV. Equations and quadric surfaces describing individual dynamics of antibody formation within a wide range of antibiotic doses and time of antibody content determination were developed by the experimental data. It was shown that within the range of the doses corresponding to the therapeutic ones in man rifampicin stimulated antibody formation.

[Effects of rifampicin and doxycycline on the production of hydrogen peroxide by macrophages].

The influence of rifampicin and doxycycline on oxidative metabolism of macrophages was estimated in vitro by production of hydrogen peroxide. It was shown that low concentrations of rifampicin and doxycycline stimulated production of hydrogen peroxide by macrophages of guinea pigs. In concentrations of 1 to 10 micrograms/ml corresponding to the mean therapeutic ones doxycycline increased both the spontaneous and zymosan-induced production of hydrogen peroxide by the macrophages.

[Effect of doxycycline on immune response in a multifactorial experiment].

Multifactorial analysis was applied to studies on the effect of doxycycline on the primary immune response to sheep erythrocytes. The experimental factors were the following: the antibiotic dose, the antigen dose and the time of the onset of the antibiotic therapy with respect to the antigen action. Polynomial statistic models describing the delayed hypersensitivity and antibody titers within wide ranges of factor values were designed by the experimental data.

[Effect of doxycycline on leukocyte adhesion in a multifactorial experiment].

The multifactorial analysis was applied to study the effect of doxycycline on leukocyte adhesion to a plastic within wide ranges of the values of experimental factors such as doxycycline concentrations and magnesium and calcium ions. The data were computer-processed and second-order equations characterizing the influence of the factors on adhesion were derived. It was shown that the effect of the ions on adhesion was non-single-valued; accurate quantitative models describing the ion effect on adhesion were developed when the concentration of doxycycline was changed.

[Effect of rifampicin and doxycycline on leukocyte chemotaxis].

It was shown experimentally that in a dose corresponding to the average therapeutic one for man rifampicin was able to stimulate in vivo directed migration of neutrophils to the inflammation foci in mice CBA. Doxycycline used in a dose corresponding to the average therapeutic one was able to activate neutrophils and to increase chemotaxis. Intraperitoneal administration of doxycycline to guinea pigs induced liberation of large counts of neutrophils with marked segmentation of the nuclei to the peritoneal cavity.

[Study of general toxic and organotropic properties of clindamycin in long-term experiments].

The action of clindamycin monohydrate on the general state and weight rise, liver and kidney functions, peripheral blood count and pathomorphological state of the viscera was studied on rats in chronic experiments. Clindamycin was administered to laboratory animals orally in doses of 50, 100, 150 and 300 mg/kg. It was shown that some adverse reactions to the drug and in particular disorders in blood coagulation and morphological changes in the intestine did not depend on its dose and were due to duration of the drug use and probable development of dysbacteriosis.

[Study of general toxic and organotropic properties of ampicillin combined with sulbactam].

The effect of sulacillin, a combination of sulbactam and ampicillin (1:2), on the functions of the liver and kidneys, peripheral blood count, cardiovascular and central nervous systems was studied in acute and chronic experiments on animals of various species. The allergenic and local irritating properties of the combination were also studied. It was shown that the combination was low toxic and the interaction of sulbactam and ampicillin by the lethal effect was additive.

[Biologic activity and tolerance of a preparation of polyunsaturated fatty acids, obtained by a microbiological route ["biopolyene"]].

Biopolyene is a mixture of ethyl ethers of polyunsaturated fatty acids isolated from biomass of Entomophthora virulenta, a mycelial fungus. Its acute and chronic toxicity was studied on rats and guinea pigs. After oral administration of the preparation in single doses exceeding 50 g/kg there were no disorders in the general state of the rats.

[Effect of rifampicin and doxycycline on the chemiluminescence of leukocytes].

The effect of rifampicin and doxycycline on spontaneous and zymosan-induced chemiluminescence of polymorphonuclear leukocytes was studied on guinea pigs. The cells were incubated in the presence of the antibiotics, washed and stimulated by zymosan. Under such conditions rifampicin in therapeutic doses of 0.

[Mg2+-dependent doxycycline-induced inhibition of leukocyte adhesion].

The method for estimating in vitro leukocyte adhesion was modified in relation to investigation of the effect of antibiotics and other pharmacological agents on cell activation. The adhesion intensity was estimated in standard 96-cavity microplates from the content of beta-hexosaminidase in the adherent cells. That made it possible to easily automatize the method by using the equipment for enzyme immunoassay.

[General toxic and organotropic properties of cefotaxime in acute and chronic experiments].

The action of cefotaxime on the functions of the liver and kidneys, the peripheral blood count, growth and development of young animals, blood circulation, respiration and the central nervous system was studied in acute and chronic experiments on mice and rats. Allergenic, immunomodulating, embryotoxic and teratogenic properties of the antibiotic were also studied. Cefotaxime was shown to be low toxic.