Assessment of the suitability of phytotoxkit plastic vertical containers compared with petri dishes for standard seedling growth tests.

This study compares two cultivation methods (Petri dishes vs. Phytotoxkit containers; tap water vs. Knop's solution) for standard seedling growth tests.

[The effect of peripheral cholinergic blockers on the cocarcinogenic action of an experimental influenza infection].

The cocarcinogenic effect of chronic influenza infection was shown to be mediated by M-cholinergic structures. Administration of a M-anticholinergic agent in low dose at the key stages of chronic influenza infection development was followed by a nearly complete suppression of its cocarcinogenic action. N-anticholinergic agents failed to exert such effect.

[The effect of emotional-pain stress syndrome on the biochemical characteristics of the stress-realizing system and metastasis of Lewis lung carcinoma in mice].

The emotional-pain stress (EPS) in C57BL mice with Lewis carcinoma was studied for its influence on serotoninergic, noradrenergic, dopaminergic, glutamatergic, aspartatergic, glycinergic, taurinergic, GABA-ergic and cholinergic mediator mechanisms of the hypothalamus, the level of corticotropin, free and bound 11-corticosteroids, insulin, thyroxin and testosterone in blood plasma as well as on the content of catecholamines, their precursors and catabolites in urine. EPS contributed to a long-term activation of stress-realizing systems resulting in generalization of decompensation in them. The stimulating effect of EPS on the incidence and growth of metastases is established depending on the terms of its application.

[Changes in neurohumoral factors of various tissues in patients with breast cancer].

Such neurohumoral factors of antitumor resistance regulation as catecholamines, corticosteroids and cholinergic mechanisms were studied in tissues of breast cancer patients. Tissue adjacent to tumor revealed elevated concentrations of 11-hydroxycorticosteroids and adrenaline matched by an increased release of norepinephrine which greatly depended on weakened cholinergic influences. These disturbances were pronounced in stages I and II tumors and, particularly, in infiltrating scirrhous cancer.

[Neurohormonal changes during the growth of metastatic Lewis carcinoma in C57Bl mice].

Functions of adrenal gland cortex, mediator system of brain and of sympathoadrenal system, including hormone formation in medullar layer of adrenal glands, were studied in mice of C57BL strain with growing metastatic Lewis carcinoma. The neurohormonal activity was distinctly inhibited under conditions of stress within the period of adaptation to the tumor development (I-14 days after the carcinoma inoculation) as well as in development of metastases (21 day of the tumor growth). Excessive stimulation of the systems studied as well as their exhaustion, might cause a distinct augmenting effect on the tumor growth and development of metastases.

[Effect of modification of the lipid bilayer on the functioning of the electron transport chain of endoplasmic reticulum membranes in nitrosodiethylamine-induced hepatic carcinogenesis].

The kinetics of cytochrome P-450 content in endoplasmic reticulum membranes at different stages of hepatocarcinogenesis was studied under the influence of unsaturated fatty acids by the EPR method. At early stages of carcinogenesis the preparations containing oleic, linoleic and arachidonic acids prevent changes in the cytochrome P-450 content usually observed in case of nitrosodiethylamine introduction. The diet containing unsaturated fatty acids has a stabilizing effect revealed by the similar changes in the content of cytochrome P-450, phosphatidyl choline and fatty acids composition and also in the level of organization of the endoplasmic reticulum membrane lipid bilayer.

[Metastasis and stress reactions in the surgical resection of tumors].

Complex investigations of brain mediator systems, catecholamine metabolism, II-oxycorticosteroid level in blood plasma, histochemical study of hormonogenesis in adrenal medulla and adrenal cortex in C57N1 mice with metastatic Lewis (3LL) carcinoma showed that metastatic stimulation initiated by the removal of a tumour caused an intensive prolonged stress reaction which differed from the reaction to a surgical trauma without removing a tumour. It is supposed that neuroendocrinal stress reactions could to a considerable extent determine this metastatic stimulation.

[Changes in the metastasis of experimental tumors and in the antimetastatic effect of cytostatics in their pharmacological action on adrenergic processes].

Different pharmacologic drugs (isadrin, obsidan, phentolamine) changing adrenergic processes are shown to have different effect on the growth and development of lung metastases. An increase in the antimetastatic effect of some cytostatics is also observed in mice with Lewis carcinoma and melanoma B-16 under the influence of isadrin, a stimulator of beta-adrenoreceptors. In authors' opinion, isadrin changes microcirculation in lungs and prevents inhibition of the immune reactivity in animals with metastatic tumours, thus contributing to inhibition of the development and growth of metastases.