Publications by authors named "Smaran Sivashankar"
Nucleic Acids Res
October 2024
Article Synopsis
- Cas9 is a powerful genome-editing tool used in biomedical research, but its interaction with mammalian cells and regulatory mechanisms is not well understood.
- Researchers identified Keap1 as an E3 ligase in mammals that targets Cas9 and its variants for degradation through a specific signal, impacting their effectiveness.
- By creating Cas9 mutants that avoid Keap1 recognition, the study shows improved gene editing capabilities and less disruption of Keap1's normal function, offering potential for more efficient gene regulation.
