Effect of Diets with Different Lipid's Sources On Serum and Brain Fatty Acids Profile: Experimental Model.

Background: The importance of diet in health is widely accepted and recognized. Diet lipid profile is important to prevent chronic diseases and improve the quality of an individual's life.

Objective: The objective of this report is to analyze the effect of different sources of dietary lipids with standard and high concentration on growing rats.

Nutritional Status and Lipid Profile in HIV-Infected Adults.

In the last decades, there have been many reports of HIV infection and abnormalities in lipid metabolism and cardiovascular disease (CVD). This study aims at describing the nutritional status of HIV-infected adults and its relation to lipid profile through traditional [total cholesterol (TC), HDL cholesterol (HDL), triglycerides (TG), non-HDL cholesterol and LDL cholesterol (LDL)] and other parameters [Apolipoprotein B (ApoB), fibrinogen, and high sensitive-C-reactive protein (hs-CRP)]. A cross-sectional descriptive study was performed.

Dietary modulation of thymic enzymes.

Malnutrition is a complex syndrome caused by an inadequate intake of energy, protein, minerals and vitamins which affects the immune system. Nutritional imbalances, present in children with energy-protein malnutrition and infections, make defining the specific effects of each of them on the thymus difficult. For this reason, it is necessary to design an experimental model in animals that could define a single variable.

Fluoxetine potentiation of omega-3 fatty acid antidepressant effect: evaluating pharmacokinetic and brain fatty acid-related aspects in rodents.

We previously reported that combined fluoxetine administration at antidepressant doses renders additive antidepressant effects, whereas non-antidepressant doses potentiate the omega-3 fatty acid antidepressant effect. In the present study, we aimed to evaluate putative pharmacokinetic and brain omega-3 fatty acid-related aspects for fluoxetine potentiation of omega-3 fatty acid antidepressant effect in rats. Coadministration of omega-3 fatty acids with a non-antidepressant dose of fluoxetine (1 mg/kg day) failed to affect both brain fluoxetine concentration and norfluoxetine plasma concentration profile.

Effects of high-fat diets from different sources on serum and thymus lipid profile: study in an experimental model.

Background: A balanced diet is important to maintain an optimal health status and to prevent noncommunicable chronic diseases. The principal objective of this study was to analyze the effect of diets containing high fat levels from different sources, on serum and thymus lipid profile, in adult rats.

Methods: Experimental diets contained 50% kcal of fat, provided by butter (B) or sunflower oil (S); control diet (C) was isocaloric, with 15 kcal of fat per 100 total kcal, provided by soy oil.

Potentiation of omega-3 fatty acid antidepressant-like effects with low non-antidepressant doses of fluoxetine and mirtazapine.

Despite the advances in psychopharmacology, the treatment of depressive disorders is still not satisfactory. Side effects and resistance to antidepressant drugs are the greatest complications during treatment. Based on recent evidence, omega-3 fatty acids may influence vulnerability and outcome in depressive disorders.

Inflammatory biomarker profile in children with cystic fibrosis: preliminary study.

The aim of this preliminary study was to determine specific proteins, related to inflammation process and nutritional status as well as to total antioxidant capacity, in children suffering from cystic fibrosis (CF). The study was performed on 17 nonhospitalized children (12 boys and 5 girls) with CF aged 3 months to 10 years, who were assisted at the Nutrition Service from Pedro de Elizalde Hospital. Transferrin, transthyretin, ceruloplasmin (Cp), haptoglobin, C-reactive protein (CRP) and fibrinogen were measured by single radial immunodiffusion techniques.

Nutrition disorder and immunologic parameters: study of the intestinal villi in growing rats.

Objective: The aim of the present work was to study how a diet in which cereals were the only protein source would affect B and T lymphocytes and a cell population positive for thymus-expressed chemokine (TECK) in the intestinal villi of growing rats.

Methods: Wistar rats were fed a 6.5% precooked maize protein diet for 18-20 d (M group).

Nutritional status in patients with HIV infection and AIDS.

The aim of this study was to evaluate the nutritional status of adults with HIV infection or with AIDS through the use of biochemical parameters. The study was performed on 43 patients (19 HIV+ and 24 AIDS patients), between 26 and 44 years of age, from low and medium socioeconomic status, with access to health care services; 35 patients were under highly active antiretroviral therapy (HAART) treatment. Body weight and height were determined, and the Body Mass Index calculated (kg/m2).

[Comparative study between two different sources of n-3 poliunsaturated fatty acids and it effect on thymus and lipid profile in rats].

In the present paper we analyzed the effect caused by different recovery diets enriched with n-3 polyunsaturated fatty acids (PUFA n-3) on thymus and serum lipid pattern. Severe depleted weanling Wistar rats (D) were divided in three groups that received during 10 days a 20% casein diet supplemented with EPA+DHA (group Cas), a 20% protein milk diet prepared using a commercial reduced-fat product enriched with linolenic and linoleic acids (group L) and a 20% casein diet as control group C. Cas and L gave each other 24 mg/day of PUFA n-3 being the ratio n-6/n-3 8.

Risk factors for cardiovascular disease in patients with anorexia nervosa.

Background: hypercholesterolemia, hypercortisolemia and low levels of essential fatty acids, oestrogens and antioxidant vitamins are more prevalent in patients with anorexia nervosa (AN) than in the general population.

Objective: This study aims (1) to compare cardiovascular risk parameters in-patients with AN and controls, and (2) to compare the parameters in-patients on admission and at four month follow up.

Methods: Blood samples and anthropometry were taken from patients with AN on admission (N=30) and matched controls (N=30).

Oral tolerance to dextrin mediated by specific suppressor T-cells induced in the intestinal intraepithelium and their systemic migration.

Antigens presented to the immune system through the oral route induce antigen specific secretory IgA and systemic unresponsiveness, termed oral tolerance (OT). We studied the induction of OT towards a diet antigen: dextrin (DEX) in rats that underwent protein deprivation and were further re-fed. Peyer's patches (PP), mesenteric lymph nodes (MLN) and spleen (Sp) cells from protein re-fed (R) rats mediated hyporesponsiveness after transfer into naïve recipient rats.

Apolipoproteins A-I and B as predictors of complications in gallbladder lithiasis surgical patients.

Specific serum proteins have been traditionally used in the assessment of protein-energy malnutrition. Some short half-life serum proteins have been related to the appearance of postoperative complications in surgical patients with low-risk pathologies that, far from undernutrition, showed a trend toward overweight and obesity. Apolipoproteins have been proposed as potential tools to assess protein status and nutritional recovery, so we investigated apolipoproteins A-I and B as new tools with prognostic value to detect postoperative complications.

Total salivary IgA, serum C3c and IgA in obese school children.

Studies of the immunologic function in adult obese humans and experimental models indicate that excess adiposity is associated with impairments in host defense mechanisms. The aim of this work was to analyze the secretory and humoral immune system in obese children (n = 105, 55 boys, 50 girls ), between 6 and 13 years of age. Samples of non-stimulated saliva and whole blood were collected from fasting patients.

Omega3 polyunsaturated fatty acids and maize flour diets.

Objectives: Nutrition disorders caused by a 6.5% maize protein diet (M), unbalanced in its indispensable amino acid pattern, provokes an arrest on cellular proliferation and maturation in the thymus of growing rats. We investigated the effect of diet supplementation with different amounts of omega3 polyunsaturated fatty acid (M = 0 mg/d, M1 = 12 mg/d, M2 = 24 mg/d, respectively) on thymus, plasma lipid concentrations, and hepatic tissue.

Nutrition disorders and immunologic parameters: study of the thymus in growing rats.

Objectives: We studied the effect of a low-quality dietary protein on cellular proliferation and maturation in the thymus of growing rats over time.

Methods: After weaning Wistar rats were fed a diet containing 6.5 g/100 g of corn flour for 6, 10, 18, and 45 d (M groups).

Activities of adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) on undernourished and renourished rats' thymus.

We studied the effect of administration of a low quality dietary protein, from weaning onwards, on the thymus of undernourished rats and the posterior effect of refeeding with a high quality dietary protein. Changes in thymus weight and the activity of Adenosine Deaminase (ADA) and Purine Nucleoside Phosphorylase (PNP) on thymus, were determined. Wistar rats were suckled in groups of 14-16 per dam since birth to weaning (23 days) to obtain undernutrition.

Potential markers of the nutritional status in an experimental model.

The activity of adenine deaminase (ADA) and purine nucleoside phosphorylase (PNP) as potential nutritional markers was analyzed in an experimental model. Weanling Wistar rats were fed a protein-free diet ad libitum to obtain a severe degree of wasting. An age-matched control group received a stock diet.

[Importance of n-3 polyunsaturated fatty acids (n-3 PUFA) in nutritional recovery].

Severe protein deprivation at weaning, may cause a stop in cellular proliferation and absolute number of T cells W3/13+ in the thymus of growing rats. The administration of a 20 g/100 casein recovery diet supplemented with 24 mg of n-3 PUFA during 9 days, counteracted this effect suggesting for these essential nutrients a dose-dependent response. No changes were observed in hemostastic factors such as protrombine time (QUICK) and partial active tromboplastine time (KPTT).

[Nutritional status of surgical patients without apparent nutritional compromise].

Nutritional status of 80 preoperative patients from programmed surgeries of hernias and lithiasis was studied by anthropometric and biochemical parameters. Nutritional deficiencies related to pathology were not expected in these patients. Results were as follows: 77% of the population showed overweight, being 15% obese.

Immunoglobulin M serum levels in females and pups of southern elephant seal (Mirounga leonina) during the suckling period.

This paper reports Immunoglobulin M (IgM) levels in serum samples from eight female-pup pairs of southern elephant seals (Mirounga leonina), at King George Island, Antarctica. IgM levels were determined on sera obtained from sequential sampling throughout the suckling period (approximately 23 days). The IgM concentration in southern elephant seal serum was measured by single radial immunodiffusion on agarose plates.

Effect of RN-301 immunomodulator on bronchus-associated lymphoid tissue (BALT) in protein depleted rats at weaning.

It has been previously demonstrated in Wistar rats that severe protein deprivation at weaning, even after refeeding with a 20% casein diet for 21 days, provokes alterations in IgA+ B cell and T cell populations from gut and GALT (gut associated lymphoid tissue) that are reverted by immunomodulator IM-104. In the present report, we investigate the influence of RN-301 (quite similar to IM-104) given by the oral or subcutaneous route during the protein deprivation period, in the seeding of BALT with IgA+ B and CD5+ T cells. The immunomodulator RN-301 contains LPS from E.

[Effect of n-3 PUFA polyunsaturated fatty acids on the thymus of rats with severe protein depletion].

Severe protein deprivation at weaning provokes an arrest of cellular proliferation and maturation, and an increase of ADA and PNP activities in the thymus of growing rats. A 20% casein diet fed during 9 days was enough to reverse the effect on ADA and PNP. The supplementation with 24 mg/d of n-3 PUFA, was able to recover thymus' cellular proliferation and maturation.