Human TAF-Iα promotes oncogenic transformation via enhancement of cell proliferation and suppression of apoptosis.

Template activating factor-I (TAF-I) is a multifunctional protein involved in various biological processes including the inhibition of histone acetylation, DNA replication, cell cycle regulation, and oncogenesis. Two main TAF-I isoforms with different N-termini, TAF-Iα and TAF-Iβ (SET), are expressed in cells. There are numerous data about functional properties of TAF-Iβ, whereas the effects of TAF-Iα remain largely unexplored.

[Dynamics of the Incubation Medium Redox Potential during Early Development Stages of Misgurnus fossilis Loach].

The analysis of kinetic curve of redox potential changes (ORP) of aqueous medium in the process of loach embryo population development at 17°C was conducted. The system of critical points defining periodization of the major stages of embryo development was identified on the ORP curve in the time interval up to τ(0) using novel methods for processing of nonlinear signals. It was shown that τ(0) was a natural periodization parameter of main morphological changes in an embryo known in the developmental biology.

[The effect of regulatory sequences of alphaS1-casein gene on the expression of the lacZ-gene in loach Misgurnus fossilis L. transgenic embryos].

Transient expression of recombinant plasmids carrying the lacZ gene under the control of either bovine alphaS1-casein gene tissue-specific promoter-enhancer region or highly homologous goat alphaS1-casein gene promoter-enhancer region with supplementary regulatory sequences of the goat gene were studied in Misgurnus fossilis L. loach embryos. It has been shown previously that the expression of the constructs carrying these regulatory elements in transgenic mice occurred primarily in the mammary glands.

[Development of loach eggs after experimental increase of cell mass in the dorsal and ventral areas of blastoderm].

The fertilized loach eggs were injected, before the beginning of cleavage, with the nuclear dye Hoechst 33258 and left to develop until the late blastula stage. Some cells of the dorsal area of stained blastoderm were transplanted in the analogous area of intact embryos of the same age, which led to an earlier and more pronounced development of head and trunk structures in recipients. A relationship was established between specific features of the development of recipients and localization of descendants of the transplanted cells.

[Loach spermatozoa transfer foreign DNA, which expression is discovered in the early development stages].

The transfer of plasmid pcDNA3-lacZ by electrotrasfected sperm cells into loach (Misgurnus fossilis L.) ova has been studied. The lacZ gene has been found to express in 3- to 5-day-old prelarvae.

[The loach--the object of experimental embryologic research in the Department of Embryology].

This paper provides a history of research at the Department of Embryology on the teleostean loach fish Misgurnus fossilis L. as a model. The use of the loach in education is briefly described.

[A cytofluorimetric study of the glycogen content and of the enzymatic activity of its metabolism in human and animal hepatocytes in liver cirrhosis and during rehabilitation].

Reversibility of hepatocyte functional activity is shown by cytofluorometric and microbiochemical methods in human and rat liver during postcirrhosis rehabilitation. Contents of the total glycogen and its fractions in liver cells were defined on smears of isolated hepatocytes obtained from the live puncture liver biopsies. A double increase of glycogen level is shown, in average, in hepatocytes during experimental liver cirrhosis in rats.

[Cytofluorimetric research on the content of glycogen and its fractions in the hepatocytes of patients with liver cirrhosis of different etiologies].

By cytofluorometric method, a study was made of the total glycogen and its two fractions in liver parenchymal cells both in the donors (20 men) and in patients with cirrhosis of different etiology (39 men). The examination was performed on preparations--smears of isolated hepatocytes, obtained from the live functional liver biopsies. The quantitative analysis has shown an increase in the total glycogen content in hepatocytes of patients with cirrhosis by 3 times compared to the norm, and this increase is independent on the etiology of liver cirrhosis.

[Integration and expression of the human somatotropic hormone gene in Teleostei].

Plasmid DNA containing human growth hormone gene was microinjected into cytoplasm of loach (Misgurnus fossilis L.) fertilized eggs. After plasmid injection, more than 50% of embryos reached the hatching stage.

[Cytotoxic properties of the splenocytes of C3HA mice following treatment with exogenous RNA].

The influence of fractions of exogenous RNA, isolated from spleens of C3HA mice and of rats, both intact (control--cRNA) and immunized with homogenate of normal syngenic, allogenic and xenogenic tissues (immune--immRNA), on the cytotoxic properties of splenocytes of C3HA intact mice was compared in the in vitro cytotoxic experiments. The splenocytes treated with different RNA fractions were used as effector-cells. In vitro cultivated MGXXIIa cells of strain specific C3HA mice hepatoma, and K562 cells of human erythroleukemia, both labeled with 3H-uridine, served as target cells.

[Chronology of the embryonic development of the common frog].

The tables of embryonic development of the common frog (Dabagyan, Sleptsova, 1975) have been made more precise: more precise timing of successive developmental stages (in the number of tau 0) from fertilization till hatching was provided and new drawings of the embryos during gastrulation were given.

[Intercellular information transfer in the process of immunogenesis. IX. The induction of the synthesis of antiphage antibodies in lymphosarcoma cells of rats as affected by different fractions of immune nuclear RNA].

Various fractions of the immune nuclear RNA were isolated from spleens of phage T2 immunized rats. The fractions were compared for their ability to induce anti-phage T2 antibody synthesis in transplantable lymphosarcoma cells. The most active proved to be the nuclear sap RNA and its subfraction with sedimentation constant of 10 S.

Effect of exogenous immunogenic RNA on the antibody synthesis in rat transplantable lymphosarcoma cells.

Cytoplasmic and nuclear RNA were isolated from sheep red cells immunized rat and mouse spleens. Rat spleen nuclear RNA was fractionated in the following steps: 1. thermal fractionation according to Georgiev, 2.

[Intercellular information transfer in the immunogenesis process. VIII. Analytical separation on agar and polyacrylamide gel of RNA fractions from the spleens of immunized and intact rats].

RNA isolated from the spleens of intact rats and from rats with immunized sheep red cells was fractionated through three steps: 1 - extraction from phenol nuclei at 50-55 degrees and 65-75 degrees C, 2 - calcium-phosphate chromatography, 3 - agar electrophoresis. Eight agar fractions were obtained of the spleens of immunized rats, an increased RNA content was manifested in at least three agar fractions: the first (4 S), the third (21 S) and the eighth (26 S) ones. The first and the eighth immune RNA fractions, as it was shown earlier, induce the synthesis of antibodies in the rat transplantable lymphosarcoma cell.

[[Intercellular information flow in the process of immunogenesis. VII. The effect of low- and high-polymer immune nuclear RNA on antibody synthesis by the cells of rat transplantable]U].

Rats were immunized with sheep red cells. From their spleen tissue 4S and 26S electrophoretically homogenous RNA fractions were extracted. Effects of these RNA fractions on the hemolysin synthesis were studied in cells of rat transplantable lymphosarcoma in the presense of actinomycin D (AD).

[Intercellular transmission of information in the process of immunogenesis. V. Study of the role of low-molecular "immune" nuclear RNA in the synthesis of antibodies by rat transplantable lymphosarcoma cells].

The karyotype of the Ararat cochineal has been studied. The cochineal chromosomes are holokinetic, i.e.

[Study of development of ciliary folds and Zinn's zonules in Rana temporaria L. by means of scanning electron microscopy].

The development of ciliary folds and zonul of Zinn has been studied in the eyes of the common frog Rana temporaria L. by means of scanning electron microscopy. The development of ciliary folds begins at the stage 45 by the flexure of the external layer in the ciliary zone.