[Aspects of the use of medical devices for remote patient monitoring].

In the Russian Federation, it is legally determined that telemedicine technologies are considered as any actions with any data obtained during a remote communication between doctors (councils, consultations), remote medical patient monitoring, including using medical devices. Medical devices with a function of remote transmission of the measurement data are used to detect diseases in some workers that impede their duty performance. There are hardly any publications on the experience of using such devices in other aspects of medical care.

Tryptophan Hydroxylase-2-Mediated Serotonin Biosynthesis Suppresses Cell Reprogramming into Pluripotent State.

The monoamine neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has important functions both in the neural system and during embryonic development in mammals. In this study, we set out to investigate whether and how endogenous serotonin affects reprogramming to pluripotency. As serotonin is synthesized from tryptophan by the rate limiting enzymes tryptophan hydroxylase-1 and -2 (TPH1 and TPH2), we have assessed the reprogramming of TPH1- and/or TPH2-deficient mouse embryonic fibroblasts (MEFs) to induced pluripotent stem cells (iPSCs).

Dual Mode of Mitochondrial ROS Action during Reprogramming to Pluripotency.

Essential changes in cell metabolism and redox signaling occur during the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). In this paper, using genetic and pharmacological approaches, we have investigated the role of electron transport chain (ETC) complex-I (CI) of mitochondria in the process of cell reprogramming to pluripotency. Knockdown of NADH-ubiquinone oxidoreductase core subunits S1 (Ndufs1) or subunit B10 (Ndufb10) of the CI or inhibition of this complex with rotenone during mouse embryonic fibroblast (MEF) reprogramming resulted in a significantly decreased number of induced pluripotent stem cells (iPSCs).

HDAC Inhibitor Sodium Butyrate Attenuates the DNA Repair in Transformed but Not in Normal Fibroblasts.

Many cancer therapy strategies cause DNA damage leading to the death of tumor cells. The DNA damage response (DDR) modulators are considered as promising candidates for use in combination therapy to enhance the efficacy of DNA-damage-mediated cancer treatment. The inhibitors of histone deacetylases (HDACis) exhibit selective antiproliferative effects against transformed and tumor cells and could enhance tumor cell sensitivity to genotoxic agents, which is partly attributed to their ability to interfere with DDR.

[The adolescents, Internet and drug abuse].

According to results of research study, in 2016-2017, the number of Internet users among schoolchildren was 96% of boys and 97% of girls and at that 44% of boys and 60% of girls spend 3 hours or more on the Internet daily. Quite often excessive infatuation for Internet is combined with psychoactive substances abuse. The purpose of the study is to identify features of abuse of drug and other toxicomanic substances among adolescents who used the Internet continuously on daily basis.

Chondrogenic differentiation followed IGFBP3 loss in human endometrial mesenchymal stem cells.

Insulin-like growth factor binding protein 3 (IGFBP3) is a multifunctional protein, able either to stimulate the cell growth or to promote apoptosis. In particular, IGFBP3 plays significant role in propagation of stress-induced senescence in human endometrium-derived mesenchymal stem cells (MESCs) (Vassilieva et al., 2020).

[The Russian rural school children of adolescent age as Internet users].

In Russia, the society shares the opinion that children, adolescents and the youth are the main users of the Internet. However, the information impact of the Internet on the emerging consciousness, intelligence, life values of younger generation is ambiguous and requires comprehensive and in-depth study. Materials and methods.

Human Alphoid Artificial Chromosome as a Gene Therapy Vector for the Developing Hemophilia A Model in Mice.

Human artificial chromosomes (HACs), including the de novo synthesized alphoid-HAC, are a powerful tool for introducing genes of interest into eukaryotic cells. HACs are mitotically stable, non-integrative episomal units that have a large transgene insertion capacity and allow efficient and stable transgene expression. Previously, we have shown that the alphoid-HAC vector does not interfere with the pluripotent state and provides stable transgene expression in human induced pluripotent cells (iPSCs) and mouse embryonic stem cells (ESCs).

Paracrine senescence of human endometrial mesenchymal stem cells: a role for the insulin-like growth factor binding protein 3.

Stress-induced premature cell senescence is well recognized to be accompanied by emerging the senescence-associated secretory phenotype (SASP). Secreted SASP factors can promote the senescence of normal neighboring cells through autocrine/paracrine pathways and regulate the senescence response, as well. Regarding human endometrium-derived mesenchymal stem cells (MESCs), the SASP regulation mechanisms as well as paracrine activity of senescent cells have not been studied yet.

Genetic tool for fate mapping of Oct4 (Pou5f1)-expressing cells and their progeny past the pluripotency stage.

Background: Methods based on site-specific recombinases are widely used in studying gene activities in vivo and in vitro. In these studies, constitutively active or inducible variants of these recombinases are expressed under the control of either lineage-specific or ubiquitous promoters. However, there is a need for more advanced schemes that combine these features with possibilities to choose a time point from which lineage tracing starts in an autonomous fashion.

Transfer of Synthetic Human Chromosome into Human Induced Pluripotent Stem Cells for Biomedical Applications.

Alphoid-type human artificial chromosome (HAC) has been recently synthetized as a novel class of gene delivery vectors for induced pluripotent stem cell (iPSC)-based tissue replacement therapeutic approach. This HAC vector was designed to deliver copies of genes into patients with genetic diseases caused by the loss of a particular gene function. The alphoid-HAC vector has been successfully transferred into murine embryonic stem cells (ESCs) and maintained stably as an independent chromosome during the proliferation and differentiation of these cells.

Immortalized murine fibroblast cell lines are refractory to reprogramming to pluripotent state.

To date different cell types of various mammalian species have been reprogrammed to induced pluripotent stem cells (iPSCs) using Yamanaka's cocktail of transcription factors (Oct4, Klf4, Sox2, and cMyc). It has been shown that several primary human cancer cell lines could be reprogrammed to iPSCs. We sought if immortalized mouse fibroblast cell lines could also be reprogrammed to iPSCs.

Role of peptidases of the intestinal microflora and prey in temperature adaptations of the digestive system in planktivorous and benthivorous fish.

Many fish enzymatic systems possess limited adaptations to low temperature; however, little data are available to judge whether enzymes of fish prey and intestinal microbiota can mitigate this deficiency. In this study, the activity of serine peptidases (casein-lytic, mainly trypsin and hemoglobin-lytic, mainly chymotrypsin) of intestinal mucosa, chyme and intestinal microflora in four species of planktivorous (blue bream) and benthivorous (roach, crucian carp, perch) was investigated across a wide temperature range (0-70 °C) to identify adaptations to low temperature. At 0 °C, the relative activity of peptidases of intestinal mucosa (<13%) and usually intestinal microflora (5-12.

Universal spatial correlation functions for describing and reconstructing soil microstructure.

Structural features of porous materials such as soil define the majority of its physical properties, including water infiltration and redistribution, multi-phase flow (e.g. simultaneous water/air flow, or gas exchange between biologically active soil root zone and atmosphere) and solute transport.

[Effect of temperature on proteinase activities of enteral microbiota and intestinal mucosa of fish of different ecological group].

Effect of temperature on proteinases activities of enteral microbiota and of intestinal mucosa was studied in five fish species (roach Rutilus rutilus, crucian carp Carassius carassius, common perch Perca fluviatilis, pike-perch Zander lucioperca, and pike Esox lucius) belonging by the nutrition type to different ecological groups. Essential differences of temperature characteristics of proteinases of intestinal mucosa and of enteral microbiota are revealed in fish belonging by the nutrition type to different ecologic groups. The character of the t0-function of proteinases of intestinal mucosa and enteral microbiota by casein and hemoglobin as a rule is different.

[The secondary structure of peptides derived from the third intracellular loop of the serpentine type receptors and its interrelation with their biological activity].

We and other authors have shown that synthetic peptides corresponding to regions of the third intracellular loop (ICL-3) of receptors of the serpentine type are capable of activating G-protein signaling cascades and trigger them in the absence of hormone. To create on the basis of these peptides the selective regulators of hormonal signaling systems the relationship between their biological activity and secondary structure are studied. It is assumed that most suitable is a helical conformation, which allows the peptide effectively interact with signaling proteins.

Influence of pH upon the activity of glycosidases and proteinases of intestinal mucosa, chyme and microbiota in fish.

It is shown that amylolytic and proteolytic activity of the intestinal mucosa, the chyme and the intestinal flora in the fishes, zander Zander lucioperca (L.), perch Perca fluviatilis L., bream Abramis brama (L.

[Sanitary and hygienic expert examination of consumer goods: toxicological aspects].

The paper presents the results of toxicological and sanitary-chemical studies of domestic and imported products subject to sanitary-and-epidemiological assessment. It shows the groups of products that are currently the greatest potential danger to health in terms of toxicological and hygienic parameters. Recommendations are given on programs for manufacturing control over the observance of sanitary rules and the antiepidemic (preventive) actions at the enterprises engaged in the production and turnover of social consumer goods.

[Experience in toxicological monitoring in Moscow].

A technology for the toxicological monitoring of chemical poisoning cases in Moscow is described. Its results over 2002-2006 are presented. The distribution of poisonings by causes, gender, and age, pattern, and occurrence circumstances is analyzed.

[Effect of temperature on proteinase activities in the intestinal chyme and the mucosa of various ecological fish groups].

Effect of temperature on activities of proteinases in intestinal chyme and mucosa was studied in three fish species (pike-perch, zope, roach) belonging to different ecological groups by their type of feeding. There was revealed a significant difference of dependence of enzyme activities in chyme on temperature in the benthophage roach (a higher level of relative activity in the zone of lower temperatures and a larger zone of temperature optimum) as well as of values of apparent energy of activation of the protein hydrolysis process as compared with that in plankto- and ichthyophages--zope and pike-perch, which indicates a significant effect of the enteral microbiota proteinases and of nutrition objects on characteristics of hydrolases functioning in fish intestine.

[Prevalence of smokers in Moscow Region].

The results of monitoring of bad habits (smoking) among senior schoolchildren in 23 towns of Moscow Region are dynamically described in the paper for 2000-2003. Smoking-related specific features are analyzed with respect to the age and sex differences of teenagers. A database, comprising above 25,000 entries, was created on the prevalence of smokers among city schoolchildren.

[Features of drug addiction among adolescent schoolchildren in the Russian Federation].

Prevalence of smoking, alcohol and drug addiction among school adolescents living in 21 towns of Russian Federation has been obtained in the anonymous questionnaire survey performed in 1991-1992. The investigated cities were divided into groups according to the nearness of the prevalences by the Average Linkage Cluster Analysis. There is dependence of strain acuteness regarding individual substance use components (alcohol, cigarette, narcotic use) in the cities on the spread of loyal attitude of the social environment to the relevant negative habits.

[Substantiation of MAC of crotonaldehyde in environmental air].

The reflex and resorptive effects of crotonaldehyde was studied. The maximum acceptable concentration (MAC) of crotonaldehyde was established to be 0.025 mg/m3, the mean daily MAC recommended to be 0.