[Dynamics of mental and physical capacities of Moscow's schoolchildren administered vitamins].

Mental and physical capacities were studied in schoolchildren aged 6-10, 11-13 and 14-17 years in the autumn-spring period during vitamin administration of "Undevitum". The six-month vitamin administration produced a positive effect on the functional state of the schoolchildren that was expressed in the improvement of their mental and physical capacity parameters.

[Oxidative modification of cytochrome P-450 during its function. II. Study of the mechanism of cytochrome P-450 LM2 inactivation in a soluble reconstructed monooxygenase system].

Inactivation of cytochrome P-450 LM2 induced by hydrogen peroxide formed in the active site of the enzyme was studied. Catalase did not protect cytochrome P-450 LM2 from inactivation during its operation in a soluble reconstituted system. The hemoprotein inactivation in this system was found to depend on the ratio of hemo- to flavoproteins.

[Reconstruction of a membrane monooxygenase cytochrome P 450-containing system in the liver using detergents in solution].

Emulgen 913, Triton N-101 and sodium cholate were compared for their reconstituting action on the dimethylaniline N-demethylation system containing cytochrome P-450 and NADPH-cytochrome P-450 reductase. The comparison showed that emulgen 913 is the most efficient detergent. The optimum molar ratio of the proteins and emulgen appeared to be equal to 1:1:600.

[Change in the activity of the microsomal system of liver oxidation in rabbits in experimental atherosclerosis].

Oxidizing ability of rabbit liver tissue was studied in experimental atheromatosis, while the functional activity of microsomal enzymatic system was evaluated by content of cytochromes P-450 and b5 as well by estimation of the reductase activity and the rates of oxidation of aniline, dimethylalanine, p-nitroanisole and amidopyrine with NADN and NADPH as cosubstrates. In rabbits with atheromatous impairment of aorta all the patterns studied were decreased by 30-70%. Severity of the aorta impairment correlated directly with the liver tissue deterioration and the rate of alteration in activity of the microsomal system oxidation constituents.

[Conformational state and functional activity of NADPH-dependent cytochrome P-450 reductase isolated and incorporated into phospholipid bilayers].

It was shown that the alpha-helix content in both isolated and incorporated into phospholipid bilayer NADPH-dependent cytochrome P-450 reductase is 20%. NADPH or dithionite reduction of the flavoprotein is not followed by conformational changes. The incorporation of the NADPH-dependent cytochrome P-450 reductase molecule into the phospholipid bilayer does not affect its catalytic properties.

[Influence of heliotrin on the rat liver microsomal oxidation system].

Content and functional activity of rat liver microsomal haemoproteins were studied after injection of heliotrin (HT). Heliotrin, at a dose of 30 mg/100 g, decreased distinctly cytochrome P-450 concentration in microsomes and increased the rate of inactivation of the enzyme reduced form. Decrease in the NADPH and NADH-dependent flavoprotein activities, decrease in O-dealkilating and p-hydroxylating activities and a distinct increase in NADPH- and ascorbate-dependent peroxidation of membrane lipids were found in rat liver microsomes treated with HT.