Analysis of Results of Specific IgE in 100 Atopic Dermatitis Patients with the Use of Multiplex Examination ALEX2-Allergy Explorer.

Background And Aim: Progress in laboratory diagnostics of IgE-mediated allergy is the use of component-resolved diagnosis. Our study analyses the results of specific IgE to 295 allergen reagents (117 allergenic extracts and 178 molecular components) in patients suffering from atopic dermatitis (AD) with the use of ALEX2 Allergy Explorer.

Method: The complete dermatological and allergological examination, including the examination of the sensitization to molecular components with ALEX2 Allergy Explorer testing, was performed.

Quality of Life after Reconstructive Surgery for Intestinal Fistulas.

Background: This retrospective clinical study would like to objectively denote a quality of life of persons afflicted by an abdominal catastrophe and managed by an extensive surgery can be almost as well conformable as those of healthy people in a similar age group.

Methods: A set of eighteen patients who were successfully surgically treated and cured enjoyed a relatively good convalescence after their surgery and returned to a satisfactory standard of life from the point of view of organ function and psychosomatic state. Statistical analysis of the data collected over a period of 1 to 6 years after this complex therapy using special questionnaire for QOL assessment SF-36 was performed.

Deproteinization is Necessary for the Accurate Determination of Ammonia Levels by Glutamate Dehydrogenase Assay in Blood Plasma From Subjects With Liver Injury.

Objective: To determine the effect of presence of high concentrations of nicotinamide adenine dinucleotide (NADH)- and nicotinamide adenine dinucleotide phosphate (NADPH)-consuming enzymes on the accuracy of glutamate dehydrogenase (GLDH) assay for ammonia.

Methods: We measured ammonia concentrations using GLDH and NADH or NADPH in blood-plasma specimens and specimens deproteinized by sulfosalicylic acid from CCl4-treated or control rats. The nonspecific oxidation of NADH and NADPH was measured in mixtures without GLDH.

Enhanced Glutamine Availability Exerts Different Effects on Protein and Amino Acid Metabolism in Skeletal Muscle From Healthy and Septic Rats.

Background: Enhanced glutamine (GLN) intake may affect the catabolism of branched-chain amino acids (BCAAs; valine, leucine, and isoleucine), which play a regulatory role in protein turnover. We examined the effects of enhanced GLN availability on leucine oxidation, amino acid concentrations, and protein metabolism in muscles from healthy and septic rats.

Methods: Cecal ligation and puncture were used as a model of sepsis.

Effect of alanyl-glutamine on leucine and protein metabolism in irradiated rats.

The mechanism by which glutamine produces a favorable effect in the treatment of sepsis, injury, burns and abdominal irradiation is not completely understood. The main aim of this study was to evaluate the effect of alanyl-glutamine (AlaGln) administration on the metabolism of proteins in irradiated rats. The rats were exposed to whole-body irradiation (8Gy) and then fed intragastrically with a mixture of glucose and amino acids either with AlaGln or without AlaGln.

Effect of alanyl-glutamine on leucine and protein metabolism in endotoxemic rats.

Background: Branched-chain amino acids (BCAA; valine, leucine, and isoleucine) have a regulatory effect on protein metabolism and are the main donor for synthesis of alanine and glutamine in the skeletal muscle. This study was performed to investigate whether exogenous alanine or glutamine would affect leucine and protein metabolism in intact and endotoxemic rats.

Methods: Rats were injected with endotoxin of Salmonella enteritidis or saline.

Use and evaluation of the Czech version of the SF-36 questionnaire self-reported health status of medical students.

SF-36 questionnaires were completed by 231 medical students of the Faculty of Medicine in Hradec Králové (1997, 1998). Results of measurements of eight health dimensions are presented here. Significantly lower values for bodily pain were found in the group of overweight students.

[Growth of the neurocranium].

The present study demonstrates the results of evaluation of growth changes of the neurocranium in a set of 98 human skulls of immature individuals aged 6-18/20/ years from the XIII.-XVIII. century.

Plasma amino acid levels after carbon tetrachloride induced acute liver damage. A dose-response and time-response study in rats.

The aims of the present study were to assess the changes of individual plasma amino acid levels in relation (1) to the severity of liver damage and (2) to the process of liver recovery. Acute liver injury was induced by an intragastric administration of CCl4 diluted in olive oil in doses of 2, 4 and/or 6 g of CCl4 per kg b.w.

Children pain during dental treatment.

This research work was done on the set of 69 children and adolescents 6-14 years old at the children's department of the dental clinic, university hospital in Hradec Králové. We found their expectancy of dental pain inadequate to reality: 67% children overestimated expected pain, 12% underestimated it. It does not see that children feelings prior to very performance would signalize in advance how much unpleasant or painful the dental procedure is going to be.

Effect of supplementation of University of Wisconsin solution with glycoproteins from psychrophilic strains of yeast on hypothermic liver storage of rats.

Extracellular yeast glycoproteins (YG) produced by Rhodosporidium toruloides have been shown to increase the survival rate of different yeast species after storage in liquid nitrogen. The purpose of this study was to investigate the effect of YG on cold-stored rat livers. Water-soluble YG produced either by Phaffia rhodozyma (G3) or by Leucosporidium antarcticum (G4) were added to a modified University of Wisconsin solution (mUW) and used for cold storage (1 degree C) of isolated livers.

A Czech version of the inventory of learning processes.

A Czech version of the Schmeck's ILP inventory was administered to 1898 university and college students from Bohemia, Moravia and Slovakia. Factor analysis identified six factors which explained 73% of data variance. The Czech version is shorter as compared to the original and includes 49 items; its reliability is satisfactory.

Proportional changes in the facial part of the skull of immature individuals.

The present study demonstrates the results of an evaluation of selected dimensions on the upper part of the splanchnocranium for a set of 98 skulls of immature individuals aged 6-18 (20) years, from the XIII.-XVIII. century.

The value of bone marrow biopsy in the prognosis of hairy cell leukemia (HCL).

Histological findings in the bone marrow and their changes in the course of the disease and therapy were evaluated with respect to the prognosis in a group of 32 patients with hairy cell leukemia (HCL). Two types of bone marrow infiltration by hairy cells (HCs), the diffuse type and the interstitial type, respectively were found. The diffuse type of infiltration and the minimal or absent residual hematopoiesis (RH) at presentation were found with statistical significance to be unfavorable prognostic findings when compared with interstitial infiltration and persisting RH (p < 0.

[Epidemiologic aspects of neoplasms and prognosis of their incidence in the Czech Republic].

"In this article development of the incidence [of] and mortality [from] neoplasms in the Czech Republic during [the] forty year period (1951-1989) has been analyzed." The authors assess the influence of cigarette smoking, high alcohol consumption, and poor nutrition. Projections for the years 1995 and 2000 are included.

[Evaluation of the quality of a decision-making rule in discriminant analysis].

Logistic regression (LR) and concept of the beta-confidence allocation rule were applied to predict the survival of dogs within the radiobiological experiment. The biochemical and haematological investigations in 86 dogs on the 2nd, 5th and 8th day post-external gamma-neutron irradiation (4.8-7.

[Comparison of the quality of diagnostic approaches using the characteristic (ROC) curve test].

The method is described to compare the quality of two diagnostic approaches as based on comparing surfaces under the ROC test curve. Apart from small deviations, this method may be applied on independent samples and paired observations carried on identical individuals. Experimental data are issued from comparing two methods of prediction of the survival in dogs on the radiobiologic experiment.

[Incidence of dental caries and dental calculus in the front dentition].

The attempt had been made to determine interrelations in the incidence of dental caries and dental of frontal dentition in a total of 100 healthy individuals aged 21-40 years. Caries susceptibility was evaluated as averagely higher in the upper frontal denture and lower in lower one. Both supragingival and subgingival forms of dental calculus were of rare occurrence in upper portion, and they occurred massively in lower portion of denture.

[General medication in elderly people].

A total of 460 elderly people (252 residents of home for aged, 137 patients of geriatric clinic and 71 old persons living in one-man households) were analysed for a general and long-term medication. They were ranged as follows: 8.5% without medication, 6.

[Comparison of stomatological findings in geriatric population of both the homes for the aged and one-man households].

In 187 residents of houses for the aged and 80 old persons living in one-man households in Hradec Králové, the authors proceeded by comparing the functional state of dentition, rate of pathological changes on the oral mucosa, level oral hygiene and regularity of stomatological care. Both groups showed no significant difference in numbers of male or female subjects, in age distribution nor in the educational level. Stomatological findings were more favorable in those living in their homes.

[Reasons for extraction of permanent teeth in a health community dental centre].

The authors assessed the diagnostic reasons for extraction of 1170 teeth in 565 patients treated in a health community dental centre. They recorded data on the patient's sex and age, on the extracted tooth and reason for extraction. They found that under the age of 30 years permanent teeth were extracter mostly on account of extensive damage by caries and its sequelae.

[Use of linear logistic regression in classification of observations].

In the present paper the prediction method using the logistic regression is explained, and the range of problems for its use is deliminated. The example is presented of mentioned method's application on how to predict the dog survival in a radiolobiological experiment. The obtained results are compared with the prediction of outcome using the linear discriminant function.

[Classification of multidimensional observations using linear discriminant functions].

In the present paper the role of linear discriminant analysis is delimitated all along with a range of problems which could be solved using this method in appropriate conditions. The analysis is given of possibilities on how to involve the decrease in number of variables in a model, and how to predict probability of incorrect classification. As an example of application, the prediction of dog survival is calculated in radiobiological experiment on the basis of biochemical and hematological values which were obtained by the 2nd day after the irradiation as well as an error probability was determined.

[Comparison of the characteristics of aged people in geriatric institutions in the Eastern Bohemia Region].

The representative trial has been performed in four types of East Bohemian geriatric institutions. Following totals were investigated: 193 patients of the long-term care institutions, 492 subjects residing in homes for aged, 96 in homes for pensionaries and 105 in homes with day-care. Various social and health characteristics have been scrutinized as follows: age, sex, residence, employment, reason for the admission, duration of stay, subjective complaints, vision and hear disorders, speech defects, denture status, incontinency, mobility, self-sufficiency, occurrence of chronical diseases, complexive evaluation of somatic and psychic conditions as well as the dependence on environs.

[Use of multidimensional data analysis in the processing of results from a gerontological study].

Relationship between the examined signs were searched for in a group of 428 persons selected in retirement dormitories in the East-Bohemian Region by means of cluster analysis of variables and factor analysis of chosen quantities. The stage of physical health, self-sufficiency, activity, adaptation, idea of the stay in the dormitory and cooperation with the staff are associated one with another. The age and data concerning social situation prior to entering the pensioners' dormitory (living conditions, education, occupation) have probably no direct effect on main characteristics of persons living there.