Application of combined Box-Behnken design with response surface methodology and desirability function in optimizing pectin extraction from fruit peels.

Background: This study aimed to extract pectin from various fruit peels, namely apple, orange, green citron, and pomegranate, and to evaluate the effects of various experimental conditions. The extraction was conducted at temperatures of 50-90 °C, with a solid-liquid ratio of 0.03-0.

Response surface methodology combined Box-Behnken design optimized green kinetic spectrophotometric and HPLC methods to quantify angiotensin receptor blocker valsartan in pharmaceutical formulations.

The high-performance liquid chromatographic (HPLC) and kinetic spectrophotometric methods were established to compute valsartan (VAL) in pharmaceutical formulations. The spectrophotometric procedures adopted initial rate, fixed time, and equilibrium strategies to assess VAL. The method was based on the carboxylic acid group of the oxidized VAL with a mixture of potassium iodate (KIO) and potassium iodide (KI) at room temperature, producing a stable, yellow-coloured absorb at 352 nm.

Development and Validation of Chromatographic and Spectrophotometric Methods for the Quantitation of Rufinamide in Pharmaceutical Preparations.

Objectives: Two optimized and validated high performance liquid chromatography (HPLC) and spectrophotometric methods are proposed. The developed methods were quantified with high sensitivity, accuracy, and precision at low concentrations to determine rufinamide (RUF) in active pharmaceutical ingredients (API) and pharmaceutical preparations.

Materials And Methods: HPLC method was developed using a base deactivated silica Hypersil C column and a combination of methanol: acetonitrile: water (15: 10: 75, v/v/v) as the mobile phase and detected at 210 nm.

Optimized Box-Behnken experimental design based response surface methodology and Youden's robustness test to develop and validate methods to determine nateglinide using kinetic spectrophotometry.

Selective and straightforward kinetic spectrophotometric methods were developed to quantify nateglinide (NTG) in pharmaceutical dosage forms. Fixed time (ΔA) and the equilibrium methods utilized the reaction of NTG with 1-chloro-2,4-dinitrobenzene (CDNB) in dimethyl sulfoxide (DMSO) with heating at 80 °C for 25 min to form a stable yellow-coloured Meisenheimer complex, which absorbs maximally at 421 nm. The optimization was achieved by utilizing the Box-Behnken experimental design (BBD) combined with response surface methodology (RSM).

V483A: an emerging mutation hotspot of SARS-CoV-2.

One of the many mutations that have occurred in the viral genome is the V483A mutation, which is a part of the receptor-binding motif present in the S1 domain of the spike protein. V483A mutant virus is popular in North America with 36 cases so far and frequently occurring in recent days. This review compares the wild-type and the V483A mutants to analyze certain factors like the interaction between the virus and host-cell interface, binding affinity, stability, partition energy, hydrophobicity, occurrence rate and transmissibility.

A comprehensive review about SARS-CoV-2.

The coronavirus disease (COVID-19) was first identified in China, December 2019. Since then, it has spread the length and breadth of the world at an unprecedented, alarming rate. Severe acute respiratory syndrome coronavirus (SARS-CoV)-2, which causes COVID-19, has much in common with its closest homologs, SARS-CoV and Middle East respiratory syndrome-CoV.

Simultaneous Determination of Potassium Clavulanate and Amoxicillin Trihydrate in Bulk, Pharmaceutical Formulations and in Human Urine Samples by UV Spectrophotometry.

A simple and sensitive UV spectrophotometric method was developed and validated for the simultaneous determination of Potassium Clavulanate (PC) and Amoxicillin Trihydrate (AT) in bulk, pharmaceutical formulations and in human urine samples. The method was linear in the range of 0.2-8.

A sensitive spectrophotometric method for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples.

A simple and sensitive spectrophotometric method was developed and validated for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples. The method was based on the reaction of drug with the mixture of potassium iodate and potassium iodide. The method was linear in the range of 0.

A novel spectrophotometric method for the determination oxacillin sodium.

A simple and sensitive spectrophotometric method was developed for the determination of Oxacillin sodium. The method was based on charge transfer complexation reaction of the drug with iodine in methanol - dichloromethane medium. The absorbance was measured at 365 nm against the reagent blank.

Development and Validation of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples by UV Spectrophotometry.

A simple and sensitive UV spectrophotometric method was developed and validated for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples. The method was linear in the range of 0.5-5.

A Validated Method without Derivatization for the Determination of Gabapentin in Bulk, Pharmaceutical Formulation and Human Urine Samples.

A rapid, sensitive and accurate high performance liquid chromatography with UV detection method was developed and validated for the quantification of gabapentin in bulk, pharmaceutical formulation and human urine samples. Most of the published methods for analysis of gabapentin used derivatization with reagent. The present paper however describes the analysis of gabapentin without any derivatization.

Development and validation of a new HPLC method for the determination of gabapentin.

A simple HPLC method was developed and validated for quantitation of gabapentin in pure form. The HPLC separation was achieved on a C18 5 μm Waters column (150 mm × 4.6 mm) using a mobile phase of methanol - potassium dihydrogen orthophosphate solution (20:80, v/v) containing 10% NaOH to adjust pH6.

Spectrofluorimetric determination of labetalol hydrochloride in pharmaceutical preparations and urine samples.

Two simple and sensitive spectrofluorimetric methods have been developed for the determination of labetalol (LBT). In method A, the native fluorescence was measured at 432 nm after excitation at 312 nm. The second method (method B) is based on the formation of a ternary complex between zinc (II), eosin and LBT.

Optimized and validated spectrophotometric methods for the determination of enalapril maleate in commercial dosage forms.

Four simple, rapid and sensitive spectrophotometric methods have been proposed for the determination of enalapril maleate in pharmaceutical formulations. The first method is based on the reaction of carboxylic acid group of enalapril maleate with a mixture of potassium iodate (KIO(3)) and iodide (KI) to form yellow colored product in aqueous medium at 25 +/- 1 degrees C. The reaction is followed spectrophotometrically by measuring the absorbance at 352 nm.