Target Bioconjugation of Protein Through Chemical, Molecular Dynamics, and Artificial Intelligence Approaches.

Covalent modification of proteins at specific, predetermined sites is essential for advancing biological and biopharmaceutical applications. Site-selective labeling techniques for protein modification allow us to effectively track biological function, intracellular dynamics, and localization. Despite numerous reports on modifying target proteins with functional chemical probes, unique organic reactions that achieve site-selective integration without compromising native functional properties remain a significant challenge.

Design, Synthesis, and Characterization of Polyoxotungstate-Decorated Ionic Liquid-Based Hybrid Material, [BmIm][SiWO] toward Rapid Adsorption of Dye and Antibacterial Activities.

A multifunctional polyoxometalate-ionic liquid (POM-IL)-based hybrid material comprising silicotungstic acid, [BmIm][SiWO], has been synthesized and demonstrated its efficiency toward methylene blue removal and as an antibacterial agent. Single-crystal XRD analysis confirms that the material crystallizes in monoclinic symmetry (SG: ), with lattice parameters = 13.1396(5) Å, = 16.

Plant-based natural products in cancer therapeutics.

Various cells in our body regularly divide to replace old cells and dead cells. For a living cell to be growing, cell division and differentiation is highly essential. Cancer is characterised by uncontrollable cell division and invasion of other tissues due to dysregulation in the cell cycle.

CuAsO: Design, Hydrothermal Synthesis, Crystal Structure, Photocatalytic Dye Degradation, Hydrogen Evolution Reaction, Knoevenagel Condensation Reaction, and Thermal Studies.

CuAsO has been explored as a heterogeneous catalyst in the fields of photocatalysis, electrocatalysis, and solvent-free organic transformation reactions. The homogeneity has been successfully attained for the first time by designing a pH-assisted hydrothermal synthesis technique. Single-crystal X-ray diffraction studies reveal that no phase transition has been observed by lowering the temperature up to 103 K with no existence of satellite reflections.

Topological reorganization and functional alteration of distinct genomic components in gallbladder cancer.

Altered three-dimensional architecture of chromatin influences various genomic regulators and subsequent gene expression in human cancer. However, knowledge of the topological rearrangement of genomic hierarchical layers in cancer is largely limited. Here, by taking advantage of in situ Hi-C, RNA-sequencing, and chromatin immunoprecipitation sequencing (ChIP-seq), we investigated structural reorganization and functional changes in chromosomal compartments, topologically associated domains (TADs), and CCCTC binding factor (CTCF)-mediated loops in gallbladder cancer (GBC) tissues and cell lines.

Oligonucleotide nanoassemblies with allyl bromide scaffold-based small molecules.

The development of oligonucleotide nanoassemblies with small molecules has shown great potential in bio-medical applications. However, the interaction of negatively charged oligonucleotides with halogenated small molecules represents a scientific challenge. Here, we introduced a distinct allyl bromide halogenated scaffold, which exhibits specific interaction with adenine nucleic bases of the oligonucleotides, thus leading to the formation of self-assembled nanostructures.

ZnSbOF and KI-Doped ZnSbOF: A Metal Oxyfluoride System for Photocatalytic Activity, Knoevenagel Condensation, and Bacterial Disinfection.

ZnSbOF crystallites were synthesized by a pH-regulated hydrothermal synthetic approach, while doping on ZnSbOF by KI was performed by the "incipient wetness impregnation technique." The effect of KI in ZnSbOF is found with the changes in morphology in the doped compound, i.e.

An Overview of Epigenetic Methylation in Pancreatic Cancer Progression.

Over the past decades, the aberrant epigenetic modification, apart from genetic alteration, has emerged as dispensable events mediating the transformation of pancreatic cancer (PC). However, the understanding of molecular mechanisms of methylation modifications, the most abundant epigenetic modifications, remains superficial. In this review, we focused on the mechanistic insights of DNA, histone, and RNA methylation that regulate the progression of PC.

Molecular Engineering of Aptamer Self-Assemblies Increases Stability and Targeted Recognition.

Functionally modified aptamer conjugates are promising tools for targeted imaging or treatment of various diseases. However, broad applications of aptamer molecules are limited by their instability. To overcome this challenge, current strategies mostly rely on covalent chemical modification of aptamers, a complicated process that requires case-by-case sequence design, multiple-step synthesis, and purification.

Assessment of Likelihood Ratio for Four Contact Dermatitis Symptoms of Vinca Minor.

Background: Contact dermatitis (CD) is a frequently occurring medical condition, for which (VM) is one of the recommended homeopathic medicines. However, the symptoms indicating this medicine have not yet been assessed systematically. Likelihood ratio (LR), based on Bayesian statistics, may yield better estimation of a medicine's indication than the existing method of entry of symptoms into materia medica and repertories.