[H-C]-NMR-Based Metabolic Kinetics Reveals Brain Neurochemical Alterations in Mice After Retinal Ischemia-Reperfusion Injury.

Retinal ischemia-reperfusion injury (RIRI) is a pathological process that occurs in various blinding eye diseases and is often accompanied by anxiety and depression. However, the underlying metabolic mechanism of mood disorders remains unclear. This study aimed to investigate the metabolic dynamics of the brain after RIRI.

Holistic parameter sensitivity: A new perspective to address the risk brought by multi-source deep uncertainty on watershed management.

Watershed models are widely applied to support watershed management, but their applications are commonly hindered by the presence of multi-source deep uncertainty (MSDU). To mitigate the impact on watershed management, this study categorizes deep uncertainties into three sources and develops a holistic sensitivity analysis to quantitatively characterize changes in parameter sensitivity influenced by MSDU. The method has been applied in Upper Chishui River Basin with (Soil and Water Assessment Tool) SWAT model, and the results indicate that the holistic sensitivity indices (HSI) of parameters show spatial heterogeneity and are significantly affected by MSDU, with the maximum increase and decrease in sensitivity indicators reaching 73.

Chongrenside D from Smilax china L protects against inflammation-induced joint destruction via inhibiting osteoclastogenesis.

Background: Bone-destructive diseases including rheumatoid arthritis (RA), osteoporosis, and bone metastases, are increasingly prevalent and worrisome due to the over-activated of osteoclasts. Chongrenside D (CGD) is a furostanol saponin extracted from Smilax china L, which has been demonstrated to have anti-inflammatory properties in our previous research. However, its effect on rheumatoid arthritis, especially on osteoclast differentiation and bone destruction has not yet been investigated.

Platelet-rich Fibrin Membrane Transplantation for the Treatment of Highly Myopic Macular Hole Retinal Detachment.

Introduction: Highly myopic macular hole retinal detachment (MHRD) is often associated with a poor prognosis, and there is currently no optimal treatment. Platelet-rich fibrin (PRF), an autologous blood product, has been shown to promote tissue regeneration. This prospective, randomized, controlled study investigated the efficacy of conventional internal limiting membrane (ILM) peeling versus PRF membrane transplantation in highly myopic MHRD.

RNA-binding proteins potentially regulate the alternative splicing of cell cycle-associated genes in proliferative diabetic retinopathy.

RNA-binding proteins (RBPs) contribute to the pathogenesis of proliferative diabetic retinopathy (PDR) by regulating gene expression through alternative splicing events (ASEs). However, the RBPs differentially expressed in PDR and the underlying mechanisms remain unclear. Thus, this study aimed to identify the differentially expressed genes in the neovascular membranes (NVM) and retinas of patients with PDR.

ATF3-CBS signaling axis coordinates ferroptosis and tumorigenesis in colorectal cancer.

The induction of ferroptosis is promising for cancer therapy. However, the mechanisms enabling cancer cells to evade ferroptosis, particularly in low-cystine environments, remain elusive. Our study delves into the intricate regulatory mechanisms of Activating transcription factor 3 (ATF3) on Cystathionine β-synthase (CBS) under cystine deprivation stress, conferring resistance to ferroptosis in colorectal cancer (CRC) cells.

METTL17 coordinates ferroptosis and tumorigenesis by regulating mitochondrial translation in colorectal cancer.

Ferroptosis, an iron-dependent lipid peroxidation-induced form of regulated cell death, shows great promise as a cancer therapy strategy. Despite the critical role of mitochondria in ferroptosis regulation, the underlying mechanisms remain elusive. This study reveals that the mitochondrial protein METTL17 governs mitochondrial function in colorectal cancer (CRC) cells through epigenetic modulation.

Taking full advantage of 'low-quality' datasets in watershed modeling and management: From a perspective of parameter calibration.

The quality of calibration datasets is critical for establishing well-calibrated models for reliable decision-making support. However, the analysis of the influence of calibration dataset quality and the discussion on how to use flawed and/or incomplete datasets are still far from sufficient. An evaluation framework for the impact of model calibration data on parameter identifiability, sensitivity, and uncertainty (ISU) was established.

Big Data-Enabled Analysis of Factors Affecting Medical Expenditure in the Cerebral Infarction of a Developing City in Western China.

Purpose: Cerebral infarction (CI) has been one of the leading causes of death in China since 2017, and controlling the medical expenses of this disease is an urgent issue for the Chinese government. This study aims to explore the important factors that affect the hospitalization expenses of CI patients and to provide a scientific basis for establishing a reasonable reimbursement mechanism and hospitalization expense standard for CI patients.

Methods: Data from 109,314 inpatients from the Healthcare Security Administration of Chengdu in western China from January 2016 to December 2018 were utilized.

Asperosaponin VI facilitates the regeneration of skeletal muscle injury by suppressing GSK-3β-mediated cell apoptosis.

Asperosaponin VI (ASA VI) is a bioactive triterpenoid saponin extracted from Diptychus roots, of Diptyl, and has previously shown protective functions in rheumatoid arthritis and sepsis. This study investigates the effects and molecular mechanisms of ASA VI on skeletal muscle regeneration in a cardiotoxin (CTX)-induced skeletal muscle injury mouse model. Mice were subjected to CTX-induced injury in the tibialis anterior and C2C12 myotubes were treated with CTX.

CREG Protects Retinal Ganglion Cells loss and Retinal Function Impairment Against ischemia-reperfusion Injury in mice via Akt Signaling Pathway.

Purpose: The irreversible death of retinal ganglion cells (RGCs) plays an important role in the pathogenesis of glaucoma. Cellular repressor of E1A-stimulated genes (CREG), a secreted glycoprotein involved in cellular proliferation and differentiation, has been shown to protect against myocardial and renal ischemia-reperfusion damage. However, the role of CREG in retinal ischemia-reperfusion injury (RIRI) remains unknown.

Wastewater reuse and energy saving require a more decentralized urban wastewater system? Evidence from multi-objective optimal design at the city scale.

Decentralization is recognized as an emerging solution for a more sustainable urban wastewater system (UWS) for the future. However, the debate of centralization vs. decentralization at the system's planning stage remains unresolved, mainly due to the complexity of the system's spatial structure and the multiple design objectives, such as water reuse and energy conservation.

Evaluation of uncertain signals' impact on deep reinforcement learning-based real-time control strategy of urban drainage systems.

Real-time control (RTC) is a recognized technology to enhance the efficiency of urban drainage systems (UDS). Deep reinforcement learning (DRL) has recently provided a new solution for RTC. However, the practice of DRL-based RTC has been impeded by different sources of uncertainties.

A Novel Bioflocculant Produced by MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae.

A preliminary study was carried out to optimize the culture medium conditions for producing a novel microbial flocculant from the marine bacterial species . The optimal glucose, yeast extract, and glutamate contents were 30, 10, and 2 g/l, respectively, while the optimal initial pH of the culture medium was determined to be 8. Following response surface optimization, the maximum bioflocculant production level of 1.

What Promotes Medical Overuse: Perspective on Evolutionary Game between Administration and Medical Institutions.

Medical overuse is the leading cause of high expenditure among healthcare systems worldwide, with the degree varying from region to region. There is increasing evidence to indicate that in China, National Healthcare Security Administration (NHSA) supervision plays the most crucial role in decreasing medical overuse. For medical overuse, traditional studies focus on empirical researches and qualitative analysis, most of which ignore how the two important participants, i.

The structure design of integrated urban drainage systems: A view of robust optimization.

Rapid urbanization puts a lot of pressure on urban water pollution from point and non-point sources, calling for the practical, specific, and integrated management of urban drainage systems (UDS). The structural design of an integrated UDS is essential for highly complex and uncertain urban water management. In this study, we developed a multi-objective robust optimization model to explore the optimal structures of UDS considering system uncertainty.

Exploring the discharge characteristics of personal care behaviors for high precision estimation of microplastic emission.

Microplastics (MPs) from personal care and cosmetic products (PCCPs) pose a threat to aquatic environment due to the small size and the complexity of composition. Although the habits of personal care behaviors (PCBs) vary from individuals affecting MPs emissions, quantitative research is not sufficient to support high-precision emission estimates and targeted management decisions. Based on a questionnaire survey about PCBs and laboratory experiments on corresponding PCCPs utilization, this study proposed a bottom-up micro-simulation method to quantify MPs emissions combining multinomial logit model and categorized emission coefficients, and identified the impact of individual attributes on the emissions.

Asperosaponin VI protects mice from sepsis by regulating Hippo and Rho signaling pathway.

Objective: To explore the novel protective effect of Asperosaponin VI (AVI) on sepsis and its potential mechanism.

Methods: In in vitro experiments, bone marrow mononuclear cells and THP-1-derived cells were used to evaluate the viability of AVI treatment. Besides, the quantitative real-time PCR and Western blot were adopted to explore the protective effect of AVI on LPS-induced inflammation.

Application of Platelet-Rich Fibrin Transplantation for Large Macular Hole.

Large idiopathic macular hole (MH) has a lower closure rate and poor visual prognosis. To increase the closure rate and the visual outcome in patients with large MH, we evaluate a technique of platelet-rich fibrin (PRF) membrane transplantation for the treatment of large MH. This was a prospective interventional study.

Machine learning approaches for the prediction of postoperative complication risk in liver resection patients.

Background: For liver cancer patients, the occurrence of postoperative complications increases the difficulty of perioperative nursing, prolongs the hospitalization time of patients, and leads to large increases in hospitalization costs. The ability to identify influencing factors and to predict the risk of complications in patients with liver cancer after surgery could assist doctors to make better clinical decisions.

Objective: The aim of the study was to develop a postoperative complication risk prediction model based on machine learning algorithms, which utilizes variables obtained before or during the liver cancer surgery, to predict when complications present with clinical symptoms and the ways of reducing the risk of complications.

Synthesis of Bitopic Ligands as Potent Dopamine D Receptor Agonists.

In this study, we designed and synthesized twelve bitopic ligands as dopamine D receptor (D R) agonists. The forskolin-induced cAMP accumulation assay revealed that all the finial compounds are able to activate D R. Furthermore, bitopic ligand N-((trans)-4-(((2,3-dihydro-1H-inden-2-yl)(propyl)amino)methyl)cyclo-hexyl)-1H-pyrrolo[2,3-b]pyridine-2-carboxamide (11 b) showed 21-fold higher potency than lead compound propyl aminoindane (2) and 17-fold higher subtype selectivity for D R over D R, indicating that the optimal length of spacer affects the D R functionality.

Cost Control of Treatment for Cerebrovascular Patients Using a Machine Learning Model in Western China.

Background: Cerebrovascular disease has been the leading cause of death in China since 2017, and the control of medical expenses for these diseases is an urgent issue. Diagnosis-related groups (DRG) are increasingly being used to decrease the costs of healthcare worldwide. However, the classification variables and rules used vary from region to region.

Risk factors associated with postoperative complications after liver cancer resection surgery in western China.

Objectives: Postoperative complications increase the workload of nursing staff as well as the financial and mental distress suffered by patients. The objective of this study is to identify clinical factors associated with postoperative complications after liver cancer resection surgery.

Methods: Data from liver cancer resections occurring between January 1st, 2019 to December 31st, 2019 was collected from the Department of Liver Surgery in West China Hospital of Sichuan University.