The feasibility of batch-wise polyester degradation and recycling using recombinant Escherichia coli expressing PHB depolymerase (PhaZ).

This study investigates the feasibility of PhaZ (PHB depolymerase derived from Caldimonas manganoxidans) in developing the PHB degradation and recycling process. PhaZ can be efficiently expressed and secreted at an OD of 0.5 and using 0.

Effects of Transcutaneous Electrical Acupoint Stimulation on the Incidence of Hypoxia in Elderly Patients Undergoing Painless Gastrointestinal Endoscopy: A Randomized Controlled Trial.

Hypoxia is not uncommon in elderly patients during painless gastrointestinal endoscopy. This study aimed to determine the effectiveness of transcutaneous electrical acupoint stimulation (TEAS) in reducing the occurrence of hypoxia symptoms in elderly patients. Patients were randomly and equally grouped into sham control ( = 109) or TEAS group ( = 109) by using the random number table method.

A preliminary study of further attempt at the development, testing and application of an auxiliary equipment for electronic colonoscopy.

Colonoscopy is a valuable tool for colorectal cancer screening and health checkups, with increasing utilization annually. Assisted entry is a standard procedure during electronic colonoscopy. In China, most clinically assisted colonoscopy procedures involve a nurse directly applying abdominal pressure to the patient's abdomen.

Latent profile analysis of spousal information concealment in patients with cancer: A cross-sectional study.

Objective: This study aimed to investigate spousal information concealment in patients with cancer and analyse its influencing factors.

Methods: Between April and July 2024, 371 spouses of patients with cancer were surveyed using the Demographic and Clinical Characteristics Questionnaire, the Information Concealment Scale for Caregivers, Fear of Progression Questionnaires for Partners, and the Medical Coping Modes Questionnaire. Influencing factors were analysed using latent profile analysis.

Bacteriocin Mining in PCZ4 with Broad-Spectrum Antibacterial Activity and Its Biopreservative Effects on Snakehead Fish.

Some lactic acid bacteria (LAB) produce antibacterial substances such as bacteriocins, making them promising candidates for food preservation. In our study, PCZ4-a strain with broad-spectrum antibacterial activity-was isolated from traditional fermented kimchi in Sichuan. Whole-genome sequencing of PCZ4 revealed one chromosome and three plasmids.

Effectiveness of nasogastric tube decompression in mitigating delayed bleeding following endoscopic submucosal dissection for early gastric cancers.

Background And Aims: Nasogastric tube decompression (NGTD) has been a standard practice following endoscopic submucosal dissection (ESD) for early gastric cancers (EGCs) in clinical settings for several years. Nevertheless, there remains a dearth of research assessing the efficacy of NGTD in preventing delayed bleeding after ESD.

Methods: From January 2021 to December 2022, eligible patients who underwent gastric ESD for EGCs were categorized into two groups: NGTD group and no NGTD group.

[Electroacupuncture preconditioning alleviates podocyte injury via PTEN/PI3K pathway in type 2 diabetes rats].

Objectives: To observe the effect of electroacupuncture (EA) preconditioning on podocytes and phosphatase and tension homologous protein (PTEN)/phosphoinositide-3-kinase (PI3K) signaling pathway in rats with type 2 diabetic kidney injury, so as to explore its potential mechanisms.

Methods: Fifty male Wistar rats were randomly divided into control, model, EA, inhibitor, and sham EA groups, with 10 rats in each group. Diabetes model was established by high-fat and high-sugar diet and intraperitoneal injection of streptozotocin (40 mg/kg).

[C(NH)]SO: A SBBO-Like Dithionate Crystal with Large Optical Anisotropy.

Investigating ultraviolet (UV) birefringent crystals is a focal point of research in recent years. The development of superior birefringent materials faces substantial challenges, primarily due to the need to pinpoint optimal fundamental building blocks and to perfect their geometric arrangement within the crystal lattice. By selecting a planar π-conjugated [C(NH)] group and a staggered [SO] group, we have successfully synthesized an organic-inorganic hybrid crystal, [C(NH)]SO.

Summary of the best evidence for risk stratification of exercise rehabilitation in patients with a cardiac implantable electronic device.

Background: Hierarchical management of sports risk is highly critical to ensure the safety of sports rehabilitation. Early identification, timely prevention and control of sports-related risk factors, and enhanced supervision and guidance can provide a basis for the formulation of sports programmes and the setting of sports monitoring levels.

Objective: This study aimed to retrieve, evaluate, and integrate evidence for the stratified management of motor risk in patients with a cardiac implantable electronic device (CIED).

Toxicological interactions of cosmetic and personal care additives mixtures: An update based on measurement and simulation.

The toxicity of chemical mixtures may be misestimated, as the assessment of individual chemicals may not adequately reflect their combined toxic effects. However, numerous combinations of chemicals and various interactions make it impossible to measure all possible mixtures. Computational toxicology can help to mitigate this issue, particularly with new methodologies that rely upon alternatives to animal testing.

Advances of M1 macrophages-derived extracellular vesicles in tumor therapy.

Extracellular vesicles derived from classically activated M1 macrophages (M1 EVs) have shown great potential in both tumor treatment and drug delivery. M1 EVs inherit specific biological messengers from their parent cells and possess the capability to activate immune cells residing in close or distant tumor tissues for antitumor therapy. Moreover, M1 EVs are commonly used as an attractive drug delivery system due to their tumor-targeting ability, biocompatibility, and non-toxic.

A pilot clinical trial of exercise program for elderly patients with cirrhosis and frailty: comprehensive exercise rehabilitation intervention.

Background And Aim: Comprehensive exercise rehabilitation has the potential to increase muscle mass and performance by stimulating protein synthesis and accelerating muscle catabolism. We developed the comprehensive exercise rehabilitation intervention (CERI) for elderly patients with cirrhosis, and we aimed to evaluate the safety and efficacy of CERI.

Methods: Eligible were elderly patients with cirrhosis and frailty.

Effusol ameliorates ischemic stroke by targeting NLRP3 protein to regulate NLRP3 inflammasome-mediated pyroptosis.

Background: The significance of pyroptosis as an inflammatory mode of death in ischemic stroke (IS) has attracted much attention in recent years. Effusol is a dihydrophenanthrene component extracted from Juncus effusus L..

Dynamics of Synovial Fluid Markers Following Single-Stage Exchange and Debridement, Antibiotics, and Implant Retention Procedure With Topical Antibiotic Infusion in Treating Periprosthetic Joint Infection.

Background: Periprosthetic joint infection (PJI) is a severe complication following total joint arthroplasty. This study aimed to investigate the dynamics of synovial fluid markers following single-stage exchange arthroplasty or debridement, combined with antibiotics and implant retention with topical antibiotic infusion for PJI.

Methods: This retrospective study analyzed patient records at a tertiary hospital from March 1, 2018, to May 1, 2023.

Protective Role of Indole-3-Acetic Acid Against Typhimurium: Inflammation Moderation and Intestinal Microbiota Restoration.

Indole-3-acetic acid (IAA), a metabolite derived from microbial tryptophan metabolism, plays a crucial role in regulating intestinal homeostasis. However, the influence and potential applications of IAA in the context of animal pathogen infections remain underexplored. This study investigates the prophylactic effects of IAA pretreatment against typhimurium (ST) SL1344 infection, focusing on its ability to attenuate inflammatory responses, enhance intestinal barrier integrity, inhibit bacterial colonization, and restore colonic microbiota dysbiosis.

Progress in gasless endoscopic thyroidectomy.

Gasless endoscopic thyroidectomy obviates the necessity for carbon dioxide insufflation to establish a surgical workspace, thus mitigating the potential complications associated with this practice. This technique presents several benefits, such as the maintenance of neck functionality, minimal scarring, and enhanced visibility of the surgical field, which contribute to its extensive adoption in clinical settings. The objective of this study is to synthesize the current methodologies of gasless endoscopic thyroidectomy and to evaluate the advantages and disadvantages inherent to each technique.

miR-155 mediated regulation of PKG1 and its implications on cell invasion, migration, and apoptosis in preeclampsia through NF-κB pathway.

Background: Preeclampsia (PE) is a severe pregnancy complication characterized by complex molecular interactions. Understanding these interactions is crucial for developing effective therapeutic strategies.

Methods: This study applies a pharmacometabolomics approach to explore the roles of miR-155 and PKG1 in PE, focusing on the regulatory influence of the NF-κB signaling pathway.

Quasiperiodic Moiré Reconstruction and Modulation of Electronic Properties in Twisted Bilayer Graphene Aligned with Hexagonal Boron Nitride.

Twisted van der Waals systems have emerged as intriguing arenas for exploring exotic strongly correlated and topological physics, with structural reconstruction and strain playing essential roles in determining their electronic properties. In twisted bilayer graphene aligned with hexagonal boron nitride (TBG/h-BN), the interplay between the two sets of moiré patterns from graphene-graphene (G-G) and graphene-h-BN (G-h-BN) interfaces can trigger notable moiré pattern reconstruction (MPR). Here, we present the quasiperiodic MPR in the TBG/h-BN with two similar moiré wavelengths, wherein the MPR results from the incommensurate mismatch between the wavelengths of the G-G and G-h-BN moiré patterns.

Atomic-Scale Insights into Surface Instability in Halide Perovskites.

The pathway of surface structure evolution plays a vital role in determining the stability of the halide perovskites. Understanding the mechanism of surface instability and how external stimuli interact with the surface is essential for developing strategies to mitigate the degradation of halide perovskites. Here, we directly observed structural evolutions on the surface of CsPbBr via an integrated differential phase contrast scanning transmission electron microscope.

How hand-made affects consumers' perceived nutritional quality of organic food.

This study analyzed the impact of handmade in depth on consumers' perceived nutritional quality of organic food through three experiments. Experiment 1 found that handmade significantly enhanced consumers' positive perceptions of nutritional quality of organic food. Experiment 2 revealed the mediating role of handicraft cultural identity between handmade and perceived nutritional quality.

Multi-omics joint analysis reveals the mechanism underlying Chinese herbal Yougui Pill in the treatment of knee osteoarthritis.

Ethnopharmacological Relevance: Yougui Pill (YGP), originating from Jingyue Quanshu, comprises 10 traditional Chinese medicines (TCMs). This classic prescription is renowned for its ability to tonify the kidney, warm the kidney, promote yang, warm the interior, and dispel cold. YGP has proven effective in treating degenerative knee arthritis, osteoporosis, delayed fracture healing, and other orthopedic conditions, making it a widely used clinical prescription for knee osteoarthritis (KOA).

Eye of the future: Unlocking the potential utilization of hydrogels in intraocular lenses.

Hydrogels are distinguished by their exceptional ability to absorb and retain large volumes of water within their complex three-dimensional polymer networks, which is advantageous for the development of intraocular lenses (IOLs). Their innate hydrophilicity offers an optimal substrate for the fabrication of IOLs that simulate the natural lens' accommodation, thereby reducing irritation and facilitating healing after surgery. The swelling and water retention characteristics of hydrogels contribute to their notable biocompatibility and versatile mechanical properties.