Isolation, characterization, and genome sequencing analysis of a novel phage HBW-1 of Salmonella.

Salmonella presents a significant threat to the health of animals and humans, especially with the rise of strains resistant to multiple drugs. This highlights the necessity for creating sustainable and efficient practical approaches to managing salmonellosis. The most recent and safest approach to combat antimicrobial resistance-associated infections is lytic bacteriophages.

Natural Polysaccharide-Based Hydrogels for Accelerating Hemostasis.

Most of the existing hydrogel dressings have inadequacies in mechanical performance, biological activities, compatibility, or versatility, which results in the development of rapid, green, and cost-effective approaches for hydrogels in biochemical and biomedical applications becoming a top-priority task. Herein, inspired by the inherent bioactivity, water retention properties, and biocompatibility of natural polysaccharide hydrogels, we have prepared self-healing gels. Using polysaccharide (BSP), carboxymethyl chitosan (CMCS), and borax via borate ester linkages, we created hemostatic and self-healing Chinese herbal medicine hydrogels in varying concentrations (2.

Long-Term Stable and Multifeature Microfluidic Impedance Flow Cytometry Based on a Constricted Channel for Single-Cell Mechanical Phenotyping.

The microfluidic impedance flow cytometer (m-IFC) using constricted microchannels is an appealing choice for the high-throughput measurement of single-cell mechanical properties. However, channels smaller than the cells are susceptible to irreversible blockage, extremely affecting the stability of the system and the throughput. Meanwhile, the common practice of extracting a single quantitative index, i.

Effect of plant species on wastewater treatment performance of a subsurface vertical-flow constructed wetland with step-feeding at low temperature.

To improve the treatment performance of constructed wetlands under low-temperature conditions, this study investigated the effects of plant species on wastewater treatment performance at low temperature and the associated microbiological characteristics in a subsurface vertical-flow constructed wetland (VFCW) with step-feeding. The results showed that the redox microenvironment in the VFCW filter with step-feeding could be restored and optimized by planting appropriate species that can tolerate low temperature, ensuring a high nitrification performance for the system. Correspondingly, the abundance and activity of three functional microbes (namely nitrifiers, denitrifiers, and anammox bacteria) increased to different degrees in the system, eventually ensuring ideal nitrogen removal by the VFCW.

gen. nov., sp. nov. and gen. nov., sp. nov., two bacteria isolated from coral , and proposal of fam. nov.

The is one of the dominant bacterial phyla in corals. However, the exact taxa of those coral bacteria under the are still unclear. Two aerobic, Gram-stain-negative, non-motile rods, designated strains BMA10 and BMA12, were isolated from stony coral collected from Weizhou Island, PR China.

Characteristics of DOM and bacterial community in rural black and odorous water bodies under different dimensions.

Analysis of dissolved organic matter (DOM) composition and microbial characteristics is crucial for tracing the sources of rural black and odorous water bodies (BOWB). The aim of this study was to explore the DOM and microbial diversity and identify the primary environmental factors in BOWB from various pollution sources during different periods using EEMs-PARAFAC and Illumina sequencing. It was found that the physicochemical properties vary widely across different pollution types of BOWB, with higher overall content during the high-water period compared to the normal-water period.

A genome-wide CRISPR screening uncovers that TOB1 acts as a key host factor for FMDV infection via both IFN and EGFR mediated pathways.

The interaction between foot-and-mouth disease virus (FMDV) and the host is extremely important for virus infection, but there are few researches on it, which is not conducive to vaccine development and FMD control. In this study, we designed a porcine genome-scale CRISPR/Cas9 knockout library containing 93,859 single guide RNAs targeting 16,886 protein-coding genes, 25 long ncRNAs, and 463 microRNAs. Using this library, several previously unreported genes required for FMDV infection are highly enriched post-FMDV selection in IBRS-2 cells.

The tolerance of two marine diatoms to diurnal pH fluctuation under dynamic light condition and ocean acidification scenario.

Coastal waters undergo dynamic changes in seawater carbonate chemistry due to natural and anthropogenic factors. Despite this, our current understanding of how coastal phytoplankton respond to fluctuating pH is limited. In the present study, we investigated the physiological responses of two coastal diatoms Thalassiosira pseudonana and Thalassiosira weissflogii to seawater acidification and diurnally fluctuating pH under natural solar irradiance.

Research on the effectiveness and safety of bronchial thermoplasty in patients with chronic obstructive pulmonary disease.

Objectives: To investigate the clinical efficacy and safety of bronchial thermoplasty (BT) in treating patients with chronic obstructive pulmonary disease (COPD).

Methods: Clinical data of 57 COPD patients were randomized into the control (n = 29, conventional inhalation therapy) or intervention group (n = 28, conventional inhalation therapy plus BT). Primary outcomes were differences in clinical symptom changes, pulmonary function-related indicators, modified Medical Research Council (mMRC), 6-min walk test (6MWT), COPD assessment test (CAT) score and acute exacerbation incidence from baseline to an average of 3 and 12 months.

Rapid weaning from mechanical ventilation and bronchial thermoplasty for near-fatal asthma: A case report.

Background: The action mechanism of bronchial thermoplasty (BT) is poorly understood. Generally, patients with severe asthma who are in desperate need of treatment have relatively low baseline values. In this paper, we describe the case of an asthmatic patient who was saved by a combination of therapy and bronchial thermoplasty.

Effect of rapeseed meal degraded by enzymolysis and fermentation on the growth performance, nutrient digestibility and health status of broilers.

The purpose of this study is to investigate the nutritional changes of degraded rapeseed meal and its effects on growth performance, nutrient digestibility and health status of broilers. Raw rapeseed meal (CON), degraded by enzymolysis (protease, ERM), fermentation (, FRM) or both (DRM) were included in diets at 25% and fed to 480 yellow-feathered broilers at 22-63 d of age. Results showed that rapeseed peptide contents (≤1 kDa) were increased ( < 0.

A high-throughput microfluidic device inspired by the Wheatstone bridge principle for characterizing the mechanical properties of single cells.

The mechanical properties of single cells have been recognized as biomarkers for identifying individual cells and diagnosing human diseases. Microfluidic devices based on the flow cytometry principle, which are not limited by the vision field of a microscope and can achieve a very high throughput, have been extensively adopted to measure the mechanical properties of single cells. However, these kinds of microfluidic devices usually required pressure-driven pumps with a very low flow rate and high precision.

Formation Optimization, Characterization and Antioxidant Activity of Auricularia auricula-judae Polysaccharide Nanoparticles Obtained via Antisolvent Precipitation.

polysaccharide (AAP)-based nanoparticles (NPs) prepared via an anti-solvent precipitation approach were studied. Response surface methodology (RSM) design was carried out on the basis of single factor experiments, using average size and polydispersity index (PDI) as indicators. The optimal preparation conditions were determined to include an AAP concentration of 1 mg/mL, a pH of 8, and an anti-solvent/solvent volume ratio of 6.

A comparison study of sodium ion- and potassium ion-modified graphitic carbon nitride for photocatalytic hydrogen evolution.

It is well known that modifying graphitic carbon nitride (GCN) is an imperative strategy to improve its photocatalytic activity. In this study, Na-doped and K-doped graphitic carbon nitride (GCN-Na and GCN-K) were prepared the simple thermal polymerization of a mixture of melamine and NaCl or KCl, respectively. The structure characterization showed that both Na and K intercalation could reduce the interlayer distance of GCN and introduce cyano defects in GCN, while K apparently had a stronger influence on the structure variation of GCN.

Correlation of Activation Site and Number with the Clinical Response to Bronchial Thermoplasty.

Objective: To investigate the relation of activation site and number with clinical response to bronchial thermoplasty (BT) in refractory asthma patients.

Methods: This work included 106 consecutive refractory asthma patients completing three BT sessions in our hospital from May 2016 to May 2019. Procedure details included recording delivery sites and those in BT.

Taphonomy of microorganisms and microbial microtextures at sulfidic hydrothermal vents: A case study from the Roman Ruins black smokers, Eastern Manus Basin.

Biological activity at deep-sea hydrothermal chimneys is driven by chemotrophic microorganisms that metabolize chemicals from the venting high-temperature fluids. Understanding taphonomy and microbial microtextures in such environments is a necessity for micropaleontological and palaeoecological research. This study examines fossilized microorganisms and related microtextures in a recent black smoker from the Roman Ruins hydrothermal vent site, Eastern Manus Basin offshore of Papua New Guinea.

Global Research Trends in Pediatric COVID-19: A Bibliometric Analysis.

Background: Coronavirus disease 2019 (COVID-19) emerged in 2019 and has since caused a global pandemic. Since its emergence, COVID-19 has hugely impacted healthcare, including pediatrics. This study aimed to explore the current status and hotspots of pediatric COVID-19 research using bibliometric analysis.

Association of serum lipid peroxidation and glutathione peroxidase 4 levels with clinical outcomes and metabolic abnormalities among patients with gestational diabetes mellitus: a case-control study in the Chinese population.

Purpose: This study investigated the association of serum lipid peroxidation (LPO) and glutathione peroxidase 4 (GPx4) with gestational diabetes mellitus (GDM) and metabolic abnormalities in Chinese pregnant women.

Methods: The present case-control study was matched at a ratio of 1:1, and it recruited 132 pairs of participants at 24-28 gestational weeks. The serum LPO and GPx4 level were determined in each subject by enzyme-linked immunosorbent assay.

Adsorption behaviors and mechanisms of antibiotic norfloxacin on degradable and nondegradable microplastics.

The misuse of both antibiotics and plastics significantly increases the environmental pollution problems associated with these contaminants. Moreover, microplastics can adsorb other pollutants in the environment. However, the mechanisms of antibiotic adsorption by degradable and nondegradable microplastics are not completely understood.

A microfluidic system for precisely reproducing physiological blood pressure and wall shear stress to endothelial cells.

To reproduce hemodynamic stress microenvironments of endothelial cells is of vital significance, by which one could exploit the quantitative impact of hemodynamic stresses on endothelial function and seek innovative approaches to prevent circulatory system diseases. Although microfluidic technology has been regarded as an effective method to create physiological microenvironments, a microfluidic system to precisely reproduce physiological arterial hemodynamic stress microenvironments has not been reported yet. In this paper, a novel microfluidic chip consisting of a cell culture chamber with on-chip afterload components designed by the principle of input impedance to mimic the global hemodynamic behaviors is proposed.

Design and construction strategies to improve covalent organic frameworks photocatalyst's performance for degradation of organic pollutants.

Covalent organic frameworks (COFs) are a class of crystalline porous organic polymers. In recent years, COFs have received extensive attention in the field of photocatalytic degradation due to their large specific surface area, good thermal and solvent stability, and diverse structures. This review studies the progress of COF in the field of photocatalytic degradation, and summarizes the strategies to improve the photocatalytic activity of covalent organic frameworks, including the designs of ligands and structures.

Oxygen Vacancies and Lewis Acid Sites Synergistically Promoted Catalytic Methane Combustion over Perovskite Oxides.

An in-depth understanding of the surface properties-activity relationship could provide a fundamental guidance for the design of highly efficient perovskite-based catalysts for the control of anthropogenic methane emission. Herein, both oxygen vacancies and Co Lewis acid sites were purposely introduced on ordered macroporous LaSrCoO monolithic catalysts by one-step reduction and selective etching in oxalic acid, and their synergistic effect on methane combustion was investigated. Combined with experimental and theoretical investigations, we revealed that the positively charged Co Lewis acid sites and single-electron-trapped oxygen vacancies (Vo·) formed an active pair, which enabled an effective localized electron cloud shift from Vo· to Co.

Interfacial sp C-O-Mo Hybridization Originated High-Current Density Hydrogen Evolution.

High-current density (≥1 A cm) is a critical factor for large-scale industrial application of water-splitting electrocatalysts, especially seawater-splitting. However, it still remains a great challenge to reach high-current density due to the lack of active and stable intrinsic catalytic active sites in catalysts. Herein, we report an original three-dimensional self-supporting graphdiyne/molybdenum oxide (GDY/MoO) material for efficient hydrogen evolution reaction via a rational design of "sp C-O-Mo hybridization" on the interface.

Investigation of the Internal Structure of a Modern Seafloor Hydrothermal Chimney With a Combination of EBSD, EPMA, and XRD.

Samples from the sphalerite-dominated zone of a seafloor massive sulfide chimney, the Satanic Mills Chimney of the PACMANUS hydrothermal field, have been investigated to determine the internal macrostructure and microstructure of this zone, the phases present, and the distribution of metals. A combination of electron probe microanalysis, electron backscattered diffraction, and x-ray diffraction has been used. At the macroscale, this zone of the chimney wall is heavily porous and is comprised primarily of sphalerite, enclosing minor chalcopyrite, pyrite, and wurtzite.