The Molecular Cloning and Functional Analysis of the Gene in L.

Seabuckthorn ( Linn.) is a commonly utilized medicinal crop with various applications in the treatment of different diseases. Two particularly noteworthy nutrients in seabuckthorn fruit are seabuckthorn oil and flavonoids.

Human Acellular Amniotic Membrane as Skin Substitute and Biological Scaffold: A Review of Its Preparation, Preclinical Research, and Clinical Application.

Human acellular amniotic membrane (HAAM) has emerged as a promising tool in the field of regenerative medicine, particularly for wound healing and tissue regeneration. HAAM provides a natural biological scaffold with low immunogenicity and good anti-infective and anti-scarring results. Despite its potential, the clinic application of HAAM faces challenges, particularly with respect to the preparation methods and its low mechanical strength.

The single and combined effects of sulfamethazine and cadmium on soil nitrification and ammonia-oxidizing microorganisms.

The coexistence of antibiotics and heavy metals in soil has attracted increasing attention due to their negative effects on microorganisms. However, how antibiotics and heavy metals affect functional microorganisms related to nitrogen cycle remains unclear. The goals of this work were to explore the individual and combined effects of sulfamethazine (SMT) and cadmium (Cd), selected as target pollutants in soil, on potential nitrification rates (PNR) and ammonia oxidizers (ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB)) structure and diversity by 56-day cultivation experiment.

Microbial nutrient limitations limit carbon sequestration but promote nitrogen and phosphorus cycling: A case study in an agroecosystem with long-term straw return.

Soil fertility can be increased by returning crop residues to fields due to the cooperative regulation of microbial metabolism of carbon (C) and nutrients. However, the dose-effect of straw on the soil C and nutrient retention and its underlying coupled microbial metabolic processes of C and nutrients remain poorly understood. Here, we conducted a comprehensive study on soil nutrients and stoichiometry, crop nutrient uptake and production, microbial metabolic characteristics and functional attributes using a long-term straw input field experiment.

Self-Supplied Electron Photoelectrochemical Biosensor with PTB7-Th as a Photoelectric Material and Biotin as an Efficient Quencher.

In this work, a self-supplied electron photoelectrochemical (PEC) biosensor for sensitive determination of Pb was established by utilizing donor-acceptor (D-A)-type PTB7-Th (poly{4,8-bis[5-(2-ethylhexyl) thiophen-2-yl]benzo[1,2-,4,5-']dithiophene-2,6-diyl-alt-3-fluoro-2-[(2-ethylhexyl)carbonyl] thieno[3,4-]-thiophene-4,6-diyl}) as a photoelectric material coupled with biotin as an efficient signal quencher. Impressively, compared with the traditional PEC signal quenchers, biotin was first applied as a PEC signal quencher in this work and it effectively avoided a cumbersome preparation process, complex DNA sequence design, and extra reagent assistance and greatly simplified experimental steps, which could achieve an efficient PEC signal quenching toward PTB7-Th. In addition, the execution of a DNAzyme-assisted Pb recycling amplification reaction could release the quencher biotin, leading to the recovery of the PEC signal, thereby realizing the quantitative detection of Pb.

Structural characterization, antioxidant activity and anti-inflammatory of the phosphorylated polysaccharide from .

In this study, a novel polysaccharide (SPN) was extracted by high-temperature pressure method and purified by a DEAE-52 column and a Sephadx G-100 gel column. PPN was obtained after phosphorylation of SPN. The differences of structural features, antioxidant activity, and anti-inflammatory effect of the two polysaccharides were investigated by chemical methods and RAW 264.

Au NP-Decorated g-CN-Based Photoelectochemical Biosensor for Sensitive Mercury Ions Analysis.

Herein, an efficient and feasible photoelectrochemical (PEC) biosensor based on gold nanoparticle-decorated graphitic-like carbon nitride (Au NPs@g-CN) with excellent photoelectric performance was designed for the highly sensitive detection of mercury ions (Hg) . The proposed Au NPs@g-CN was first modified on the surface of the electrode, which possessed a remarkable photocurrent conversion efficiency and could produce a strong initial photocurrent. Then, the thymine-rich DNA (S1) was immobilized on the surface of the modified electrode via Au-N bonds.

Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From .

polysaccharides (GLP) exhibited excellent immunomodulatory activity. Unfortunately, the structure and immunomodulatory activity of GLP are still unclear. GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.

Photoelectrochemical monitoring of miRNA based on Au NPs@g-CN coupled with exonuclease-involved target cycle amplification.

Herein, a sensitive photoelectrochemical (PEC) biosensing platform was designed for quantitative monitoring of microRNA-141 (miRNA-141) based on Au nanoparticles@graphitic-like carbon nitride (Au NPs@g-CN) as the signal generator accompanying with T7 exonuclease (T7 Exo)-involved target cycle amplification process. Initially, the prepared Au NPs@g-CN as the signal generator was coated on the electrode surface, which could produce a strong PEC signal due to the unique optical and electronic properties of g-CN and the surface plasmonic resonance (SPR) enhanced effect of Au NPs. Meanwhile, the modified Au NPs@g-CN was also considered as the fixed platform for immobilization of S1-S2 through Au-N bond.

Extraction kinetics, physicochemical properties and immunomodulatory activity of the novel continuous phase transition extraction of polysaccharides from .

polysaccharides (GLP) possess remarkable bioactivity and have been studied widely. However, the application of new technologies in the polysaccharide extraction has not been investigated. Herein, a novel continuous phase transition extraction (CPTE) technology was applied for the extraction of polysaccharides from .

Influence of microplastics occurrence on the adsorption of 17β-estradiol in soil.

High levels of steroid estrogens are continuously detected in the soil environment, and even the concentration in vegetables and fruits has reached levels that have an impact on children's health, which has attracted growing attention. Moreover, microplastics (MPs) in the soil system are also of increasing concern worldwide. The effects of MPs on the adsorption of organic pollutants in soil systems, however, remain largely unexplored.

Uptake, accumulation, and translocation mechanisms of steroid estrogens in plants.

Applying sewage sludge or animal manure onto agricultural land can result in estrogen pollution, which increases the risk of human exposure to steroid estrogens (SEs) via the food chain. However, the uptake and accumulation mechanism of SEs by plants is still unclear. In this study, the uptake, accumulation, and translocation of 17β-E2, a representative SE, were investigated through a series of wheat hydroponic experiments.

Comparison of 17β-estradiol adsorption on soil organic components and soil remediation agent-biochar.

Steroid estrogen residues (SEs) in the soil have attracted growing attention because of their potential for endocrine disruption. Soil organic matter (SOM) and soil remediation agent-biochar, both have important influences on the fate of SEs in the soil environment. This study compared the adsorption of 17β-estradiol (E2) on wheat straw biochar (W-BC) and cow manure biochar (C-BC) with main SOM components including biomacromolecules (cellulose, collagen and lignin) and humic acids (HA).

Effects of freeze-thaw cycles on distribution and speciation of heavy metals in pig manure.

To understand the potential environmental influence of animal manure under freeze-thaw cycles, pig manure was used to conduct a simulation experiment to explore the effects of freeze-thaw cycles on heavy metal distribution and form transformation. Thirty cycles of freezing and thawing were performed alternately by freezing at - 18 ± 2 °C for 24 h and thawing at 20 ± 2 °C for 24 h. By a serial wet sieving procedure, manure samples were separated into different sizes of 1000, 250, 75, 38, and < 38 μm.

Influence of Ligneous Bulking Agents on the Complexation of Heavy Metals to Humic Substances Formed in Pig Manure Composting.

In order to investigate the influence of different lignin sources on humic substance formation during composting, this study selected two lignin sources, including wood sawdust and maize straw, to be co-composted with pig manure. Humic substances (HS) were characterized based on their fluorescence characteristics and complexing behaviors with heavy metals. The results showed that lignin sources, especially wood sawdust, were more conducive in promoting the formation of humic acids (HAs) than inorganic matter.