Basing target enzyme study the enantioselective bioactivity action mechanism of flusulfinam, a novel HPPD inhibitor herbicide.

Flusulfinam is a novel chiral amide herbicide widely used for controlling annual weeds in rice paddies. However, the mechanism underlying their enantioselective herbicidal activity remain unclear. Herein, it was found that flusulfinam enantiomers, similar to typical HPPD inhibitor mesotrione, reduced chlorophyll and carotenoid levels, decreased HPPD enzyme activity, and upregulated gene expression.

Comprehensive study of flusulfinam in paddy water-sediment microcosms: Enantioselective fate, degradation pathways, and toxicity assessment.

Flusulfinam, a novel chiral herbicide, demonstrates effective weed control in paddy fields. Nevertheless, a comprehensive investigation into its environmental fate in paddy systems, particularly at the enantiomeric level, remains deficient. Herein, paddy water-sediment microcosms were constructed across four sites to explore the enantiomeric behavior of flusulfinam.

Polarized pectin accumulation regulates differential hypocotyl elongation at the dark-to-light transition.

As one of the most influential environmental factors, light fundamentally shapes plant physiology and growth traits. The hypocotyl is critical for the morphological establishment of the seedling, and its length displays remarkable plasticity upon perception of changes in the light conditions. Although remodeling of the primary cell walls is well-documented to play an important role in hypocotyl growth, how the hypocotyl elongation rate is swiftly repressed at the dark-to-light transition remains elusive.

Heterogeneity in Mechanical Properties of Plant Cell Walls.

The acquisition and utilization of cell walls have fundamentally shaped the plant lifestyle. While the walls provide mechanical strength and enable plants to grow and occupy a three-dimensional space, successful sessile life also requires the walls to undergo dynamic modifications to accommodate size and shape changes accurately. Plant cell walls exhibit substantial mechanical heterogeneity due to the diverse polysaccharide composition and different development stages.

Bacterial-host adhesion dominated by collagen subtypes remodelled by osmotic pressure.

Environmental osmolarity plays a crucial role in regulating the functions and behaviors of both host cells and pathogens. However, it remains unclear whether and how environmental osmotic stimuli modulate bacterial‒host interfacial adhesion. Using single-cell force spectroscopy, we revealed that the interfacial adhesion force depended nonlinearly on the osmotic prestimulation of host cells but not bacteria.

Comprehensive study of chiral herbicide flusulfinam uptake, translocation, degradation, and subcellular distribution in rice (Oryza sativa L.).

The biological behavior of flusulfinam, a potential commercial chiral herbicide for rice, has not been well explored. Herein, the uptake of chiral flusulfinam by rice and its transport, degradation, and subcellular distribution in rice (Oryza sativa L.) were investigated.

Geometric constraint-triggered collagen expression mediates bacterial-host adhesion.

Cells living in geometrically confined microenvironments are ubiquitous in various physiological processes, e.g., wound closure.

SCAN: Spatiotemporal Cloud Atlas for Neural cells.

The nervous system is one of the most complicated and enigmatic systems within the animal kingdom. Recently, the emergence and development of spatial transcriptomics (ST) and single-cell RNA sequencing (scRNA-seq) technologies have provided an unprecedented ability to systematically decipher the cellular heterogeneity and spatial locations of the nervous system from multiple unbiased aspects. However, efficiently integrating, presenting and analyzing massive multiomic data remains a huge challenge.

SCAR: Single-cell and Spatially-resolved Cancer Resources.

Advances in sequencing and imaging technologies offer a unique opportunity to unravel cell heterogeneity and develop new immunotherapy strategies for cancer research. There is an urgent need for a resource that effectively integrates a vast amount of transcriptomic profiling data to comprehensively explore cancer tissue heterogeneity and the tumor microenvironment. In this context, we developed the Single-cell and Spatially-resolved Cancer Resources (SCAR) database, a combined tumor spatial and single-cell transcriptomic platform, which is freely accessible at http://8.

LRP1-Mediated Endocytosis May Be the Main Reason for the Difference in Cytotoxicity of Curcin and Curcin C on U2OS Osteosarcoma Cells.

Curcin and Curcin C, both of the ribosome-inactivating proteins of , have apparent inhibitory effects on the proliferation of osteosarcoma cell line U20S. However, the inhibitory effect of the latter is 13-fold higher than that of Curcin. The mechanism responsible for the difference has not been studied.

Modulation of MagR magnetic properties via iron-sulfur cluster binding.

Iron-sulfur clusters are essential cofactors found in all kingdoms of life and play essential roles in fundamental processes, including but not limited to respiration, photosynthesis, and nitrogen fixation. The chemistry of iron-sulfur clusters makes them ideal for sensing various redox environmental signals, while the physics of iron-sulfur clusters and its host proteins have been long overlooked. One such protein, MagR, has been proposed as a putative animal magnetoreceptor.

Curcin C inhibit osteosarcoma cell line U2OS proliferation by ROS induced apoptosis, autophagy and cell cycle arrest through activating JNK signal pathway.

Osteosarcoma is a kind of primary bone malignant tumors. Its cure rate has been stagnant in the past decade years. Curcin C belongs to type I ribosome inactivating proteins, extracted from the cotyledons of post-germinated Jatropha curcas seeds.

Stable FeO submicrospheres with SiO coating for heterogeneous Fenton-like reaction at alkaline condition.

In the traditional Fenton process, the efficient generation of hydroxyl radical (HO) strongly relies on an acidic circumstance and the iron ions would precipitate and form large amounts of hazardous iron-containing sludge at alkaline pH. To realize stable heterogeneous Fenton-like catalytic degradation at alkaline condition, FeO submicrospheres with SiO coating were successfully synthesized by using water glass as the silica sources via a facile ultrasound assisted method. The as-obtained FeO@SiO spheres were further used as catalysts for the Fenton-like degradation of tetracycline hydrochloride (TC).

NLRP6 self-assembles into a linear molecular platform following LPS binding and ATP stimulation.

NOD-like receptors (NLRs) localize in the cytosol to recognize intracellular pathogen products and initialize the innate immune response. However, the ligands and ligand specificity of many NLRs remain unclear. One such NLR, NLRP6, plays an important role in maintaining intestinal homeostasis and protecting against various intestinal diseases such as colitis and intestinal tumorigenesis.

Reversible rearrangement of magnetic nanoparticles in solution studied using time-resolved SAXS method.

Superparamagnetic nanoparticles have broad applications in biology and medicines. Quantitative measurements of magnetic beads in solution are essential in gaining comprehensive understanding of their dynamics and developing applications. Here, using synchrotron X-ray sources combined with well controlled magnetic fields, the results from small-angle X-ray scattering (SAXS) experiments on superparamagnetic particles in solution under the influence of external magnetic fields are reported.

Synthesis and structural characteristics of high surface area TiO aerogels by ultrasonic-assisted sol-gel method.

TiO aerogel is a unique three-dimensional porous nano-particle material with the characteristics of high specific surface area and good light transmittance. In this paper, a novel method involving ultrasonic-assisted sol-gel, solvent exchange, and vacuum drying was successfully developed to synthesis the TiO aerogel. The morphology and properties of the prepared TiO aerogels were characterized by the Brunauer-Emmett-Teller theory (BET), x-ray diffraction, field-emission scanning electron microscopy, energy dispersive spectroscopy, thermogravimetric analysis-differential thermal analysis, Fourier transform infrared spectroscopy, and Raman spectroscopy.

A magnetic protein biocompass.

The notion that animals can detect the Earth's magnetic field was once ridiculed, but is now well established. Yet the biological nature of such magnetosensing phenomenon remains unknown. Here, we report a putative magnetic receptor (Drosophila CG8198, here named MagR) and a multimeric magnetosensing rod-like protein complex, identified by theoretical postulation and genome-wide screening, and validated with cellular, biochemical, structural and biophysical methods.