Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.

Single-molecule super-resolution imaging is instrumental in investigating cellular architecture and organization at the nanoscale. Achieving precise 3D nanometric localization when imaging structures throughout mammalian cells, which can be multiple microns thick, requires careful selection of the illumination scheme in order to optimize the fluorescence signal to background ratio (SBR). Thus, an optical platform that combines different wide-field illumination schemes for target-specific SBR optimization would facilitate more precise 3D nanoscale studies of a wide range of cellular structures.

Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.

Single-molecule super-resolution imaging is instrumental for investigating cellular architecture and organization at the nanoscale. Achieving precise 3D nanometric localization when imaging structures throughout mammalian cells, which can be multiple microns thick, requires careful selection of the illumination scheme in order to optimize the fluorescence signal to background ratio (SBR). Thus, an optical platform that combines different wide-field illumination schemes for target-specific SBR optimization would facilitate more precise, 3D nanoscale studies of a wide range of cellular structures.

A Generic Strategy to Stabilize Wide Bandgap Perovskites for Efficient Tandem Solar Cells.

Efficient wide bandgap (WBG) perovskite solar cells (PSCs) are essential for fully maximizing the potential of tandem solar cells. However, these cells currently face challenges such as high photovoltage losses and the presence of phase segregation, which impede the attainment of their expected efficiency and stability. Herein, the root cause of halide segregation is investigated, uncovering a close association with the presence of locally aggregated lead iodide (PbI ), particularly at the perovskite/C interface.

Targeting GPX4-mediated Ferroptosis Alleviates Liver Steatosis in a Rat Model of Total Parenteral Nutrition.

Background: Parenteral nutrition-associated liver disease (PNALD) is a common hepatobiliary complication resulting from long-term parenteral nutrition (PN) that is associated with significant morbidity and mortality. Ferroptosis plays a significant role in the pathogenesis of various liver diseases. This study aims to explore the role of ferroptosis in PNALD and to uncover its underlying mechanisms.

Attenuates Liver Steatosis and Bile Acid Dysregulation in Parenteral Nutrition-Fed Rats.

Parenteral nutrition (PN), a vital therapy for patients with intestinal failure, can lead to the development of parenteral nutrition-associated liver disease (PNALD). In this study, we aimed to investigate the role of () in a rat model of PNALD. Total parenteral nutrition (TPN)-fed rats were used to assess the role of in liver steatosis, bile acid metabolism, gut microbiota, and hepatocyte apoptosis.

The gut microbiome and intestinal failure-associated liver disease.

Intestinal failure-associated liver disease (IFALD) is a common hepatobiliary complication resulting from long-term parenteral nutrition (PN) in patients with intestinal failure. The spectrum of IFALD ranges from cholestasis, steatosis, portal fibrosis, to cirrhosis. Development of IFALD is a multifactorial process, in which gut dysbiosis plays a critical role in its initiation and progression in conjunction with increased intestinal permeability, activation of hepatic immune responses, and administration of lipid emulsion.

Targeted metabolomics unravels altered phenylalanine levels in piglets receiving total parenteral nutrition.

Parenteral nutrition, received by many patients with intestinal failure, can induce hepatobiliary complications, which is termed as parenteral nutrition-associated liver disease (PNALD). The spectrum of PNALD ranges from cholestasis and steatosis to fibrosis and cirrhosis. Although many factors contribute to the pathogenesis of PNALD, the underlying mechanisms remain unclear.

Periodic Acid Modification of Chemical-Bath Deposited SnO Electron Transport Layers for Perovskite Solar Cells and Mini Modules.

Chemical bath deposition (CBD) has been demonstrated as a remarkable technology to fabricate high-quality SnO electron transport layer (ETL) for large-area perovskite solar cells (PSCs). However, surface defects always exist on the SnO film coated by the CBD process, impairing the devices' performance. Here, a facile periodic acid post-treatment (PAPT) method is developed to modify the SnO layer.

MAX-DOAS Measurements of Tropospheric NO and HCHO Vertical Profiles at the Longfengshan Regional Background Station in Northeastern China.

The vertical profiles of nitrogen dioxide (NO) and formaldehyde (HCHO) in the troposphere at the Longfengshan (LFS) regional atmospheric background station (127°36' E, 44°44' N, 330.5 m above sea level) from 24 October 2020 to 13 October 2021 were retrieved from solar scattering spectra by multi-axis differential optical absorption spectroscopy (MAX-DOAS). We analyzed the temporal variations of NO and HCHO as well as the sensitivity of ozone (O) production to the concentration ratio of HCHO to NO.

Emerging role of regulated cell death in intestinal failure-associated liver disease.

Intestinal failure-associated liver disease (IFALD) is a common complication of long-term parenteral nutrition that is associated with significant morbidity and mortality. It is mainly characterized by cholestasis in children and steatohepatitis in adults. Unfortunately, there is no effective approach to prevent or reverse the disease.

RNA-sequencing identifies novel transcriptomic signatures in intestinal failure-associated liver disease.

Background: Total parenteral nutrition (TPN) dependence leads to development of intestinal failure-associated liver disease (IFALD). The spectrum of diseases ranges from cholestasis, steatosis, fibrosis, and cirrhosis that causes significant morbidity. Understanding the disease at molecular level helps us to develop therapeutic targets.

Recent Progress in Intelligent Wearable Sensors for Health Monitoring and Wound Healing Based on Biofluids.

The intelligent wearable sensors promote the transformation of the health care from a traditional hospital-centered model to a personal portable device-centered model. There is an urgent need of real-time, multi-functional, and personalized monitoring of various biochemical target substances and signals based on the intelligent wearable sensors for health monitoring, especially wound healing. Under this background, this review article first reviews the outstanding progress in the development of intelligent, wearable sensors designed for continuous, real-time analysis, and monitoring of sweat, blood, interstitial fluid, tears, wound fluid, etc.

miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis.

Background: miRNAs regulate a multitude of cellular processes and their aberrant regulation is linked to human cancer. However, the role of miR-425-5p in lung cancer (LCa) is still largely unclear. Here, we explored the role of miR-425-5p during LCa tumorigenesis.

Functional DNA-based hydrogel intelligent materials for biomedical applications.

Deoxyribonucleic acid (DNA) nanotechnology is a relevant research field of nano-biotechnology, which has developed rapidly in recent years. Researchers have studied DNA far more than they have studied its genetic characteristics, and now it has evolved into the field of nanomedical materials. A variety of articles based on DNA nanostructures can be obtained by rational design and controllable preparation.

Tropospheric NO vertical column densities retrieved from ground-based MAX-DOAS measurements at Shangdianzi regional atmospheric background station in China.

Ground-basedMulti-AXis Differential Optical Absorption Spectroscopy (MAX-DOAS) measurements were performed at Shangdianzi (SDZ) regional atmospheric background station in northern China from March 2009 to February 2011. The tropospheric NO vertical column densities (VCDs) were retrieved to investigate the background condition of the Beijing-Tianjin-Hebei developed economic circle in China. The seasonal variation of mean NO tropospheric VCDs (VCD) at SDZ is apparent, with the maximum (1.

Ratios of greenhouse gas emissions observed over the Yellow Sea and the East China Sea.

During a cruise of the survey vessel Dongfanghong II on the Yellow Sea and the East China Sea in the spring of 2017 we performed accurate measurements of the mole fractions of carbon dioxide (CO), methane (CH), carbon monoxide (CO) and nitrous oxide (NO) using two types of Cavity Ring-Down Spectrometers (CRDS). The spatial variations of the mole fraction of the four trace gases were very similar. The emission sources of these gases were divided into several regions by using the NOAA HYSPLIT model.

Pathologically assessed grade of Hirschsprung-associated enterocolitis in resected colon in children with Hirschsprung's disease predicts postoperative bowel function.

Purpose: The aim of this study was to investigate the relationship between the grade of enterocolitis on pathological assessment of resected colon and postoperative bowel function in children with Hirschsprung's disease (HD).

Methods: Children with HD who were seen at a large tertiary center from January 2012 to December 2013 were enrolled into this study. Resected colon was assessed using the histopathologic grade scoring system for Hirschsprung-associated enterocolitis (HAEC), and the relationship of these scores to postoperative bowel function was assessed.

[Remote sensing of seasonal variation in column concentration of atmospheric CO2 and CH4 in Hefei].

In order to observe two kinds of greenhouse gases, CO2 and CH4, making the biggest contribution to global warming, a ground-based Fourier transform near-infrared spectral remote sensing system was developed to record the perpendicular incidence sun spectra from February 2012 to April 2013 in Hefei continuously. The measured total transmittances in the atmosphere were obtained from perpendicular incidence sun spectra. Methods of line-by-line and low-order polynomial approximation were used to model the total atmospheric transmittances in forward model.

[Quantitative analysis of nitrate in atmospheric particulates PM2.5 with Fourier transform infrared spectroscopy].

Airborne fine particulate matter PM2.5 as one of composite core pollutants of air pollution is concerned and NO as one of the main components of water-soluble ions has an important impact on precipitation and human health, so searching a method of rapid and reliable detection is an important work. According to advantages of the Fourier transform infrared spectroscopy technology, the infrared spectrum of NO3- in NH4NO3 was compared with PM2.

[Optical properties research of Bacillus subtilis spores by Fourier transform infrared spectroscopy].

The authors measured IR transmission spectra of two different concentrations of Bacillus subtilis spores by using Fourier transform infrared spectroscopy (FTIR) technology. The mass extinction cross section k of Bacillus subtilis spores was calculated according to Lambert-Beer law and the imaginary part n(i) of the complex refractive index was also calculated through k. The real part n(r) of the complex refractive index was derived from the KK (Kramers-Kronig) relationship and the experimental results were also analyzed and discussed with the study of measurement and analysis method of the complex refractive index on Bacillus subtilis spores, it is of great significance to further research the absorption and scattering characteristics, and to broaden the measurement and remote sensing technology method of the biological aerosols.

[Monitoring and analysis of urban ozone using open path Fourier transform infrared spectrometry].

An ozone monitoring system was developed by the method of open path Fourier transform infrared (OP-FTIR) spectrometry based on our FTIR spectrometer. In order to improve measurement precision and detection limit, the quantitative analysis was completed to get ozone concentration by combining synthetic background spectrum method which uses information from HITRAN database and instrumental line shape, and nonlinear least squares (NLLSQ) method. The measurement methods for system detection limit were discussed and the result is 1.

[Ozone concentration inversion based on multi-reflected cell FTIR spectra and correlation analysis].

The stratosphere ozone plays the protective action role for human and the ground-level ozone is harmful to human health. Monitoring ozone with different ways and methods took an active part in understanding distribution and transformation of ozone, which was useful to controlling pollution emission. Spectra were got by multi-reflected white cell Fourier transform infrared (FTIR) spectrometer, inversed with nonlinear least squares (NLLSQ) method and then the concentrations of ozone were got exactly.

[Concentration inversion of high temperature air from FTIR spectra and analyzing residual error structure].

The spectral line widths of theory and experiment are analyzed with different temperatures; the line strengths under room temperature in HITRAN database are corrected to measured temperature, and then synthetic spectra are calculated. With the nonlinear least squares fit between measured spectra and calibration spectra, standard gas concentrations of CO at different temperatures are obtained. The inversion concentration error of this algorithm at room temperature is less than 5% with high precision.