Traditional vs novel out-of-office method for collecting cytology and HPV DNA - a comparative study.

Objectives: The integration of high-risk human papillomavirus (HPV) detection into cervical cancer screening in Poland aims to improve early detection, yet challenges in coverage and adherence persist. Innovative approaches, like sampling for HPV testing and cytology outside medical settings, have been proposed. This study assesses the feasibility and agreement of results between traditional and novel sampling methods.

Numerical simulation for a neurotransmitter transport model in the axon terminal of a presynaptic neuron.

Neurotransmitters in the terminal bouton of a presynaptic neuron are stored in vesicles, which diffuse in the cytoplasm and, after a stimulation signal is received, fuse with the membrane and release its contents into the synaptic cleft. It is commonly assumed that vesicles belong to three pools whose content is gradually exploited during the stimulation. This article presents a model that relies on the assumption that the release ability is associated with the vesicle location in the bouton.

Silver nanoparticle monolayers on poly(ethylene imine) covered mica produced by colloidal self-assembly.

Monodisperse silver particles were synthesized according to the method of Creighton et al. by reduction of AgNO(3) solutions with NaBH(4) in the presence of polyvinyl alcohol as the stabilizing agent. Bulk characteristics of silver nanoparticles in aqueous solutions were carried out by measuring their extinction spectrum, fluorescence, diffusion coefficients using the PCS method and the electrophoretic mobilities.

Deposition of particles in the impinging-jet cell for the high coverage regime.

Particle deposition in the radial impinging-jet cell for the high coverage regime was studied theoretically and experimentally. A detailed description of the flow distribution in the cell was attained by solving the governing Navier-Stokes equation numerically. The macroscopic flow pattern was decomposed into simpler local flows.

Surface clusters of colloid particles produced by deposition on sites.

The possibility of producing surface clusters of well-defined structure formed by colloid particles was analyzed theoretically and experimentally. Theoretical results were derived by performing Monte Carlo-type simulations according to the generalized random sequential adsorption (RSA) mechanism. In these simulations, the jamming coverage of particles adsorbing irreversibly on spherical sites was determined as a function of the particle-to-site size ratio lambda.

Irreversible adsorption of particles on heterogeneous surfaces.

Methods of theoretical and experimental evaluation of irreversible adsorption of particles, e.g., colloids and globular proteins at heterogeneous surfaces were reviewed.

Irreversible adsorption of particles at random-site surfaces.

Irreversible adsorption of negatively charged polystyrene latex particles (averaged diameter 0.9 microm) at heterogeneous surfaces was studied experimentally. The substrate bearing a controlled number of adsorption sites was produced by precovering mica sheets by positively charged polystyrene latex (averaged diameter of 0.

Kinetics of particle deposition in the oblique impinging jet cell.

A new oblique impinging-jet (OBIJ) cell was developed, suitable for colloid deposition studies at various interfaces. In contrast to previously used orthogonal cells, the OBIJ construction makes possible direct microscope observations of particle deposition on nontransparent substrates. The cell performance was tested by studying kinetics of polystyrene latex particle deposition on mica.

[The evaluation of nitrates and nitrites in daily food intake of adults].

During the research work the contents of nitrates and nitrites in whole day's food of adults were examined. The study were carried out in February 1996. In the evaluation of the degree of exposure highest permissible daily intake was considered of nitrites (0.

[The evaluation of nitrate and nitrite contents in diets of preschool children].

The contents of nitrites and nitrates in whole day's food of children at preschool age have been examined. Meals consisted of three dishes were taken from one of the day nurseries in Lódź in January 1996. In the evaluation of the degree of exposure the highest permissible daily intake was considered of nitrites (0.

Total serum sialic acid concentration as a supporting marker of malignancy in ovarian neoplasia.

The present study was undertaken to establish the diagnostic utility of total sialic acid (SA) determination in the serum of patient with ovarian neoplasia. In a group of 29 patients with histopathologically confirmed ovarian neoplasia, concentrations of total SA was determined. In addition, the two tumor markers: CA125 antigen level and lactic dehydrogenase (LDH) activity were determined.

Production and regulation of interleukin-11 by breast cancer cells.

We have studied the production of interleukin-11 (Il-11) in 13 breast cancer cell (BCC) lines. Two of these cell lines (MDA-MB-231 and Hs578T) expressed the cytokine at both the protein and mRNA levels. Il-11 did not modulate the growth of five BCC lines examined, including the two cytokine-producing BCC lines.

Establishment and characterization of three new breast-cancer cell lines.

We have established and characterized 3 new breast-cancer cell lines from pleural effusions of patients with advanced breast cancer. All 3 cell lines, designated IBEP-1, IBEP-2 and IBEP-3, showed typical ultrastructural characteristics of epithelial mammary tumor cells. Electron microscopy showed, among other characteristics, the presence of numerous microvilli, desmosomal junctions, intracytoplasmic duct-like vacuoles, well-developed endoplasmic reticulum and large nuclei.

Breast cancer cell response to calcitonin: modulation by growth-regulating agents.

Calcitonin may induce cyclic AMP production by breast cancer cells and inhibit their growth. The molecular complex leading to cyclic AMP production in response to calcitonin is made of the calcitonin receptor coupled to the adenylate cyclase by at least one guanine nucleotide-binding protein (G-protein, of the Gs type). Our aim was to determine whether and how the responses of cells to calcitonin were modulated by growth-regulating agents not directly acting through the cyclic AMP pathway.

NOTE.

The adsorption kinetics of negatively charged polystyrene latex at mica surface precovered with smaller sized (submicrometer) latex particles was investigated experimentally. The direct microscope observation method combined with the impinging jet technique was used in this study. Experimental results were presented concerning the initial flux and adsorption kinetics of larger particles at surfaces partially covered with smaller sized latex particles.

Secretory products of breast cancer cells specifically affect human osteoblastic cells: partial characterization of active factors.

The pathogenesis of tumor-induced osteolysis (TIO) following breast cancer metastases in bone remains unclear. We postulated that osteoblasts could be target cells for the secretory products of breast cancer cells. We previously showed that serum-free conditioned medium (CM) of the breast cancer cell line MCF-7 inhibits DNA synthesis by 75% of control values in osteoblast-like cells SaOS-2 and that this effect is only in a minor part due to transforming growth factor beta secretion.

Characterization of non pigmented B16 melanoma cell-derived cytotoxic factors.

We analyzed and tried to characterize substance(s) responsible for cytotoxic activities detected in culture media conditioned by non pigmented B16 melanoma cells (NPB16). The different cytological tests used showed that ultrafiltrated conditioned media (CM U1 fraction) contained several cytotoxic factors with a Mw lower than 1000 Da. These factors seemed to act either directly or indirectly on cell membranes, mitochondria, on the cell cycle and on protein and DNA synthesis.

Influence of Polydispersity on Random Sequential Adsorption of Spherical Particles.

Random sequential adsorption (RSA) of polydisperse mixtures of hard and interacting spherical particles was analyzed. Theoretical results were derived by performing numerical MC simulations both for Gaussian and for continuous distributions of particle sizes characterized by standard deviations below 20%. Adsorption kinetics of these mixtures was determined for a broad range of times showing that for tau < 5 the influence of polydispersity was rather minor for both Gauss and continuous particle size distributions.

Effects of secretory products of breast cancer cells on osteoblast-like cells.

The pathogenesis of breast cancer-induced osteolysis remains largely unknown. To evaluate the potential role of osteoblasts as target cells during this process, we incubated SaOS-2 human osteoblast-like cells (OBL) with culture media conditioned by proliferative (PM, 'Proliferation Media') or confluent (CfM, 'Confluence Media') MCF-7 human breast cancer cells. CfM decreased the growth of OBL by 26% (P < 0.

[Studies on the contents of nitrates and nitrites in selected fresh and heat processed vegetables].

The levels of nitrates and nitrites were determined in fresh vegetables and the same products subjected to culinary processing such as boiling. Nitrates were reduced on a cadmium column to nitrites, where upon they were determined colorimetrically using sulfanilic acid and N-1-naphthyl-ethylenediamine. Thermal processing of these vegetables reduced the level of nitrates by about 50% and the nitrites loss reached even 100%.

[Content estimation of nitrates and nitrites in vegetables from the province of Lodz].

The contents of nitrates and nitrites in selected vegetables reaped in summer and autumn in 1993 were investigated. The samples of vegetables were collected directly from the producers from area of Lódź and from outside of the city. Nitrates were reduced on a cadmium column to nitrites, where-upon they were determined colorimetrically using sulfanilic acid and N-1-Naphthylethylenediamine.

Characterization and tumorigenicity of spheroids composed of pigmented or non pigmented B16 melanoma cells.

A parental line of mouse B16 melanoma cells (B16) and two derived cloned lines, either pigmented (B16P) or non pigmented (B16NP), were cultured in vitro as spheroids. After 48 hrs, the pigmented cells (B16, B16P) formed smaller and looser aggregates, with higher rates of cell proliferation and lower amounts of extracellular matrix as compared to B16NP spheroids. The three lines were more tumorigenic when inoculated subcutaneously as spheroids than as isolated cells.

Effect of selenium compounds on murine B16 melanoma cells and pigmented cloned pB16 cells.

The effects of selenium compounds such as sodium selenite, sodium selenate, seleno-DL-cystine and seleno-DL-methionine (100 microM and 10 microM) on B16 and pigmented cloned pB16 murine melanoma cells were investigated in vitro. At the tested concentrations, B16 cells showed a greater sensitivity to the toxic effects of sodium selenite and seleno-DL-cystine than pB16 cells, whereas no decrease of B16 and pB16 cell number was observed after incubation with sodium selenate or seleno-DL-methionine. Glutathione (GSH) percentages were strongly decreased only by selenite and seleno-DL-cystine; it was marked more in B16 than in pB16 cells.

[A case of uterine foreign body present for many years].

A clinical case of a perennial presence of fragments of fetus skull is presented in the article. Patient was treated symptomatically for 5 years for irregular menstrual cycles. Only USG examination and a probationary biopsy explained the exciting cause.

Effects of trace metals on mouse B16 melanoma cells in culture.

The effects of fourteen metal ions (As3+, As5+, Cd2+, Co2+, Cr3+, Cr6+, Hg2+, Li+, Mg2+, Mn2+, Ni2+, Se4+, V5+, VO2+) on the proliferation and differentiation in mouse B16 melanoma cells cultivated in vitro were analyzed. Cell number assays, melanin, and protein measurements, a 3(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide reduction test (MTT survival test), and a clonal growth assay were performed. At 10(-4)M, metal ions such as As3+, As5+, Cd2+, Cr6+, Se4+, V5+, VO2+, and, to a minor extent, Li+, Hg2+, and Co2+ significantly reduced the number of the B16 melanoma cells.