Polyethylene (PE) is the second most commonly used plastic worldwide, mainly used to produce single-use items such as bags and bottles. Its significant resistance to natural biodegradation results in the accumulation of PE in landfills, leading to various ecological and toxicological consequences. Despite extensive research on the microbial degradation of PE, achieving complete biodegradation remains a challenge.
View Article and Find Full Text PDFLignin, a vital plant component, is key in providing structural integrity and is the second most abundant biopolymer in nature. The growing interest in sustainable and efficient biocatalysis has driven the exploration of lignin nanoparticles (LNPs) as a promising platform for enzyme immobilization. Given lignin's abundance and structural role in plants, converting it into nanoparticles offers a potential eco-friendly alternative to traditional supports.
View Article and Find Full Text PDFThe invasive Asian clam species, Corbicula fluminea, has significant ecological and societal implications at both local and international levels due to its nutritional aspects. C. fluminea from four urban rivers in Bangladesh exhibited negative allometric growth and degree of contamination with potentially toxic elements (PTEs), which posed a concern to human health based on the AAS and USEPA risk models.
View Article and Find Full Text PDFChemotaxis, the directed movement of microbes in response to chemical gradients, plays a crucial role in the biodegradation of xenobiotics, such as pesticides, industrial chemicals, and pharmaceuticals, which pose significant environmental and health risks. Emerging trends in genomics, proteomics, and synthetic biology have advanced our understanding and control of these processes, thereby enabling the development of engineered microorganisms with tailored chemotactic responses and degradation capabilities. This process plays an essential physiological role in processes, such as surface sensing, biofilm formation, quorum detection, pathogenicity, colonization, symbiotic interactions with the host system, and plant growth promotion.
View Article and Find Full Text PDFAcne is a persistent infectious skin condition primarily caused by Propionibacterium acne that affects 80 % of teenagers. The rise of antibiotic resistance in P. acnes has led to an increasing interest in exploring alternative antimicrobial agents.
View Article and Find Full Text PDFSite-directed protein immobilization allows the homogeneous orientation of proteins while maintaining high activity, which is advantageous for various applications. In this study, the use of SpyCatcher/SpyTag technology and magnetic nickel ferrite (NiFeO NPs) nanoparticles were used to prepare a site-directed immobilization of BsUGT2m from Bacillus subtilis and AtSUSm from Arabidopsis thaliana for enhancing curcumin glucoside production with UDP-glucose regeneration from sucrose and UDP. The immobilization of self-assembled multienzyme complex (MESAs) enzymes were characterized for immobilization parameters and stability, including thermal, pH, storage stability, and reusability.
View Article and Find Full Text PDFIndustrialization and population growth are leading to the production of significant amounts of sewage containing hazardous xenobiotic compounds. These compounds pose a threat to human and animal health, as well as the overall ecosystem. To combat this issue, chemical, physical, and biological techniques have been used to remove these contaminants from water bodies affected by human activity.
View Article and Find Full Text PDFThis review delves into the mesmerizing technology of nano-agrochemicals, specifically pesticides and herbicides, and their potential to aid in the achievement of UN SDG 17, which aims to reduce hunger and poverty globally. The global market for conventional pesticides and herbicides is expected to reach USD 82.9 billion by 2027, growing 2.
View Article and Find Full Text PDFEpigenetic modifications, mainly aberrant DNA methylation, have been shown to silence the expression of genes involved in epigenetic diseases, including cancer suppression genes. Almost all conventional cancer therapeutic agents, such as the DNA hypomethylation drug 5-aza-2-deoxycytidine, have insurmountable side effects. To investigate the role of the well-known DNA protectant (ectoine) in skin cell DNA methylation and cancer cell proliferation, comprehensive methylome sequence analysis, 5-methyl cytosine (5mC) analysis, proliferation and tumorigenicity assays, and DNA epigenetic modifications-related gene analysis were performed.
View Article and Find Full Text PDFAlthough dye-decolourising peroxidases (DyPs) are well-known for lignin degradation, a comprehensive understanding of their mechanism remains unclear. Therefore, studying the mechanism of lignin degradation by DyPs is necessary for industrial applications and enzyme engineering. In this study, a dye-decolourising peroxidase (CsDyP) gene from C.
View Article and Find Full Text PDFThe consumption of contaminated finfish from the polluted river channel of Turag-Tongi-Balu, Kamarpara site, Dhaka poses significant health hazards to humans. We used mass spectrometry on chemically digested liquid samples from five fish species from Turag-Tongi-Balu to estimate the concentrations of 10 elements (Cr, Mn, Ni, Cu, Zn, As, Se, Cd, Fe, and Pb). Except M.
View Article and Find Full Text PDFGlycosyltransferases (GTs) catalyze the transfer of active monosaccharide donors to carbohydrates to create a wide range of oligosaccharide structures. GTs display strong regioselectivity and stereoselectivity in producing glycosidic bonds, making them extremely valuable in the in vitro synthesis of oligosaccharides. The synthesis of oligosaccharides by GTs often gives high yields; however, the enzyme activity may experience product inhibition.
View Article and Find Full Text PDFLaccases are powerful multi-copper oxidoreductases that have wide applicability as "green" biocatalysts in biotechnological, bioremediation, and industrial applications. Sustainable production of large amounts of functional laccases from original sources is limited by low yields, difficulties in purification, slow growth of the organisms, and high cost of production. Harnessing the full potential of these versatile biocatalysts will require the development of efficient heterologous systems that allow high-yield, scalable, and cost-effective production.
View Article and Find Full Text PDFPolylactic acid (PLA) has been used in fused deposition method (FDM) based 3D printing for many years. Alkali lignin is an undervalued industrial by-product that could upgrade PLA's poor mechanical properties. This work presents a biotechnological approach consisting of a partial degradation of alkali lignin using Bacillus ligniniphilus laccase (Lacc) L1 for its use as a nucleating agent in a polylactic acid/thermoplastic polyurethane (PLA/TPU) blend.
View Article and Find Full Text PDFLignin is essential for plant growth, structural integrity, biotic/abiotic stress resistance, and water transport. Besides, lignin constitutes 10-30% of lignocellulosic biomass and is difficult to utilize for biofuel production. Over the past few decades, extensive research has uncovered numerous metabolic pathways and genes involved in lignin biosynthesis, several of which have been highlighted as the primary targets for genetic manipulation.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
June 2022
Microbial communities composed of bacteria, archaea and fungi play a pivotal role in driving the biogeochemical cycles in the marine ecosystem. Despite the vastness of the South Indian Ocean, only a few studies reported the simultaneous analysis of bacterial, archaeal and fungal diversity therein, particularly archaeal and fungal communities in deep-sea environments received less attention previously. In this study, microbial diversity, community composition and dynamics in microbial community structure in eight deep-sea sediment samples collected from different sites at varying depths of the South Indian Ocean were explored using Next-Generation Sequencing.
View Article and Find Full Text PDFAs an abundant aromatic biopolymer, lignin has the potential to produce various chemicals, biofuels of interest through biorefinery activities and is expected to benefit the future circular economy. However, lignin valorization is hindered by a series of constraints such as heterogeneous polymeric nature, intrinsic recalcitrance, strong smell, dark colour, challenges in lignocelluloses fractionation and the presence of high bond dissociation enthalpies in its functional groups etc. Nowadays, industrial lignin is mostly combusted for electricity production and the recycling of inorganic compounds involved in the pulping process.
View Article and Find Full Text PDFThe recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role in biomass degradation, nutrient recycling and ecosystem functioning. Therefore, the use of microbes is an attractive way to transform biomass to produce clean energy and high-value compounds.
View Article and Find Full Text PDFmSystems
November 2020
has been reported as a multidrug-resistant bacterium due to biofilms and antimicrobial resistance mechanisms. Hence, novel therapeutic strategies are necessary to overcome infections. This study revealed that citral at 200 μg/ml attenuated biofilms by up to 90% without affecting viability.
View Article and Find Full Text PDFIndole and its derivatives have been shown to interfere with the quorum sensing (QS) systems of a wide range of bacterial pathogens. While indole has been previously shown to inhibit QS in , the effects of various indole derivatives on QS, biofilm formation, and virulence of remain unexplored. Hence, in the present study, we investigated the effects of 51 indole derivatives on biofilm formation, QS, and virulence factor production.
View Article and Find Full Text PDFThis study determined the antibiofilm and antivirulence potential of 5-Dodecanolide (DD) against an exclusive human pathogen Streptococcus pyogenes. Biofilm quantification assay showed antibiofilm efficacy of DD with maximum biofilm inhibition of 85% at 225 μg/mL concentration. Efficacy of antibacterial property of DD (225 μg/mL) was confirmed by CFU analysis and Alamar blue assay.
View Article and Find Full Text PDFThe biorefining technology for biofuels and chemicals from lignocellulosic biomass has made great progress in the world. However, mobilization of laboratory research toward industrial setup needs to meet a series of criteria, including the selection of appropriate pretreatment technology, breakthrough in enzyme screening, pathway optimization, and production technology, etc. Extremophiles play an important role in biorefinery by providing novel metabolic pathways and catalytically stable/robust enzymes that are able to act as biocatalysts under harsh industrial conditions on their own.
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