Publications by authors named "Sivan Tzadok"
Visualizing nuclear RNAi activity in single living human cells.
Proc Natl Acad Sci U S A
October 2017
Nuclear RNA interference (RNAi) is mediated by the canonical RNAi machinery and can lead to transcriptional silencing, transcriptional activation, or modulation of alternative splicing patterns. These effects transpire through changes in histone and DNA modifications via RNAi-mediated recruitment of chromatin-modifying enzymes. To prove that nuclear RNAi occurs and modulates transcription in human cells, we used live-cell imaging to detect and track nuclear RNAi transcriptional repression in single living human cells.
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- * Researchers are using RT-PCR to detect long noncoding RNAs (lncRNAs) that are associated with these gene silencing events, but they've encountered issues with false detection due to unexpected RT priming.
- * By improving experimental methods, such as using higher temperatures and specific treatments, the study successfully confirmed the presence and direction of lncRNAs linked to notable genes like BRMS1, RAR-β2, and CST6 in breast cancer cell lines.
Glioblastoma multiforme (GBM) is a highly aggressive malignant brain tumor. Despite some recent improvement in the treatment of this malignancy, life expectancy of GBM patients remains extremely low. Therefore, continuous efforts to develop new treatment modalities are mandatory.
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