Comparisons between ingestion, rejection, and egestion of microbeads by burrowing clams, Meretrix meretrix and Paphia undulata: Implications for health risk of shellfish consumption.

Two burrowing clam species, namely Meretrix meretrix and Paphia undulata, were offered two sizes (small: 45-53 μm, and large: 106-125 μm) of fluorescent red polyethylene microbeads, and the ingestion (number of MPs in the body tissue and faeces) and rejection (number of MPs in pseudofaeces) of MPs investigated. Overall, MP beads ingested were 36 % more than those rejected. There was also a significant interaction between the size and fate of MPs.

The influences of spatial-temporal variability and ecological drivers on microplastic in marine fish in Hong Kong.

This study examined microplastic (MP) occurrence and abundance in marine fish collected from the western and eastern waters of Hong Kong during the wet and dry seasons. Over half (57.1%) of the fish had MP in their gastrointestinal (GI) tracts, with overall MP abundance ranging from not detected to 44.

Adverse impacts of high-density microplastics on juvenile growth and behaviour of the endangered tri-spine horseshoe crab Tachypleus tridentatus.

The impacts of high-density microplastics, namely polyamine 6,6 (nylon), polymethyl methacrylate (PMMA) and polyethylene terephthalate (PET), on growth and behaviour of the endangered tri-spine horseshoe crab Tachypleus tridentatus were investigated for 100 days. Negative changes in wet weight and prosomal width of the juveniles were observed in all treatments of microplastics, but significant difference was only detected in prosomal width between control and PMMA. T.

A systematic study of microplastic occurrence in urban water networks of a metropolis.

The release of microplastics from sewage treatment works (STWs) into the oceans around coastal cities is well documented. However, there are fewer studies on the microplastic abundance in stormwater drains and their emissions into the coastal marine environment via sewage and stormwater drainage networks. Here, we comprehensively investigated microplastic abundance in 66 sewage and 18 sludge samples collected from different process stages at three typical STWs and 36 water samples taken from six major stormwater drains during the dry and wet seasons in Hong Kong, which is a metropolitan city in south China.

Macroalgal morphology mediates microplastic accumulation on thallus and in sediments.

The accumulation process of microplastics (MPs) is a key to understanding their fate in the environment. However, there is limited information about the short-term accumulation of MPs on macrophytes. The ability of macrophyte to attenuate wave and reduce current velocity is potentially facilitating MPs deposition.

Fate and Effects of Macro- and Microplastics in Coastal Wetlands.

Coastal wetlands trap plastics from terrestrial and marine sources, but the stocks of plastics and their impacts on coastal wetlands are poorly known. We evaluated the stocks, fate, and biological and biogeochemical effects of plastics in coastal wetlands with plastic abundance data from 112 studies. The representative abundance of plastics that occurs in coastal wetland sediments and is ingested by marine animals reaches 156.

The significance of trophic transfer in the uptake of microplastics by carnivorous gastropod Reishia clavigera.

The present study compared the relative significance of prey consumption and respiration as routes of microplastic (MP) intake in a carnivorous muricid gastropod, Reishia clavigera. The time-dependent accumulation of MPs within 14-day exposure and their removal through depuration were also investigated for two forms of MPs (fibre, fragment) at an environmentally relevant concentration (10 items L) and two higher concentrations (100 and 1000 items L). At 1000 items L, the number of MPs in R.

Determination of microplastics in the edible green-lipped mussel Perna viridis using an automated mapping technique of Raman microspectroscopy.

Microplastics are prevalent in marine environments and seafood and thus can easily end up in human diets. This has raised serious concerns worldwide, particularly in Hong Kong where the seafood consumption per capita can be three times higher than the global average. This study focused on the green-lipped mussel Perna viridis, a popular seafood species which is subject to a high risk of contamination by microplastics due to its filter-feeding nature.

Bisphenol A and its analogues in sedimentary microplastics of Hong Kong.

The occurrence and spatial distribution of bisphenol A (BPA) and analogues bisphenol B (BPB), bisphenol F (BPF) and bisphenol S (BPS) were investigated in microplastic on 11 beaches in Hong Kong. At 10 sites, BPA was the only detected chemical with concentrations ranged from 82.4-989 ng g microplastic.

Horseshoe crab genomes reveal the evolution of genes and microRNAs after three rounds of whole genome duplication.

Whole genome duplication (WGD) has occurred in relatively few sexually reproducing invertebrates. Consequently, the WGD that occurred in the common ancestor of horseshoe crabs ~135 million years ago provides a rare opportunity to decipher the evolutionary consequences of a duplicated invertebrate genome. Here, we present a high-quality genome assembly for the mangrove horseshoe crab Carcinoscorpius rotundicauda (1.

How mangrove plants affect microplastic distribution in sediments of coastal wetlands: Case study in Shenzhen Bay, South China.

Microplastic pollution is common in marine and coastal ecosystems, especially in mangrove wetlands. However, factors affecting the distribution of microplastics, such as plants, have not been sufficiently studied. We investigated the effect of different plant species on the distribution of sediment microplastics in two Nature Reserves in South China, viz.

Enhanced remediation of BDE-209 in contaminated mangrove sediment by planting and aquaculture effluent.

Toxic and persistent flame retardant (BDE-209) and aquaculture effluent (AE) are ubiquitous in coastal environments, but how their co-existence influences their fate is not yet investigated. This study investigated AE effects on remediation and uptake of BDE-209 by Kandelia obovata (Ko) and Avicennia marina (Am), true and dominant mangrove species. After 12-months, a significant removal of BDE-209 was achieved in planted mangrove sediment and the removal was significantly enhanced by AE addition, possibly due to the enhancement of nitrogen (N) and phosphorous (P) content in sediment.

Effects of rubble zones from oyster cultivation on habitat utilization and foraging behaviour of the endangered tri-spine horseshoe crab: An implication for intertidal oyster cultivation practices.

Estuarine structured habitats, including benthic cultivation structure, are widely cited as substantial nursery and feeding grounds for fish and macroinvertebrates. Activities such as locomotion and foraging behaviour of sediment-dwelling species, however, may be hindered in the presence of the obstacles. In this study, collected field evidence demonstrated that habitat utilization of the juvenile tri-spine horseshoe crab Tachypleus tridentatus, an iconic and endangered macroinvertebrate, was adversely affected by high-density intertidal oyster rubble zones.

Characterisation of an unexplored group of microplastics from the South China Sea: Can they be caused by macrofaunal fragmentation?

Research on plastics fragmentation is important for the estimation of amount of microplastics but the biological causes for fragmentation have not been acknowledged. From microplastics collected in the beaches of Hong Kong, we revealed an abnormal type of fragment which has not been reported before. These fragments, composing about 6% of the microplastics (pellet, foam, bead, fragment) collected, were interestingly triangular in shape with at least two of the three sides being characteristically straight and resembling a cut made by compression.

Changes of substrate microbial biomass and community composition in a constructed mangrove wetland for municipal wastewater treatment during 10-years operation.

Constructed wetlands (CWs) have been used for wastewater treatment for decades, but research on microorganisms involved, especially long-term changes, is still limited. In this study, we evaluated changes in the substrate microbial community in a pilot-scale horizontal subsurface-flow constructed mangrove wetland during 10-years operation. In the 3rd year of operation, microbial biomass carbon and phospholipid fatty acids (PLFAs) reached peak values in two vegetated belts planted with Aegiceras corniculatum (Ac) and Kandelia obovata (Ko), respectively, then stabilized or declined in the 9th and 10th years of operation.

Heavy metals contamination of sedimentary microplastics in Hong Kong.

This is the first study of heavy metals (As, Zn, Cd, Ni, Fe, Mn, Cu) contamination of microplastics on sandy beaches in Hong Kong. Three study sites are located in the eastern waters (Pak Lap Wan, Stanley Bay, Tung Lung Chau) and the other three in the western waters (Tai Pai Tsui, Ha Pak Nai, Shui Hau Wan). The three most abundant types of microplastics were polyethylene (42.

Impacts of Typhoon Mangkhut in 2018 on the deposition of marine debris and microplastics on beaches in Hong Kong.

Storm surge and waves associated with tropical cyclones carry significant amounts of pollutants into the marine environment. This study evaluated the effects of Typhoon Mangkhut (7-18 September 2018) on marine debris pollution including macro-debris (>2.5 cm) and microplastics (5 μm-5 mm) in Hong Kong.

Field test of beach litter assessment by commercial aerial drone.

The visual survey is the most common method to quantify and characterize beach litter. However, it is very labor intensive and difficult to carry out on beaches which are remote or difficult to access. We suggest an alternative approach for assessing beach litter using an unmanned aerial vehicle (UAV), or aerial drone, with automated image requisition and processing.

Microplastics in invertebrates on soft shores in Hong Kong: Influence of habitat, taxa and feeding mode.

Microplastic (MP) pollution in the marine environment has gained much concern in recent years. This study investigated the occurrence of MPs in invertebrates collected on 18 mudflats and sandy beaches in Hong Kong and its relationships to biological taxon, feeding mode and habitat. In total 38 species of gastropods, bivalves and crabs were collected and the mean number of suspected microplastics ranged from 0 to 9.

Development of a digestion method for determining microplastic pollution in vegetal-rich clayey mangrove sediments.

Microplastics are ubiquitous pollutants found in environments. Mangrove sediments containing vegetal litter are different from other environmental matrices such as river and marine sediments. The presence of vegetal litter leads to an under-estimation of microplastic pollution, particularly classical digestion methods are not suitable for removing this type of organic matter.

Effects of aquaculture effluents on fate of 2,2',4,4',5-pentabromodiphenyl ether (BDE-99) in contaminated mangrove sediment planted with Kandelia obovata.

The problems of aquaculture effluent (AE) and polybrominated diphenyl ethers (PBDEs) are common in coastal areas. The fate of 2,2',4,4',5-pentabromodiphenyl ether (BDE-99), a dominant PBDE congener, in mangrove sediments and the effects of AE on it have never been reported. A 12-months microcosm study was conducted and more than 55% of the BDE-99 in contaminated sediment was removed at the end.

Could mangrove plants tolerate and remove BDE-209 in contaminated sediments upon long-term exposure?

Polybrominated diphenyl ethers (PBDEs) such as BDE-209, the commonest congener, are known to be toxic. A 24-months study using mangrove mesocosms with mixed mangrove species, namely Avicennia marina (Am), Aegiceras corniculatum (Ac) and Kandelia obovata (Ko), or without any plant was conducted to examine toxicity, removal, translocation and uptake of BDE-209. At month 24, BDE-209 stimulated the production of root superoxide radical (O), and leaf and root malondialdehyde (MDA) of Ko, enhanced leaf O of Ac, but did not affect the production of O and MDA in Am.

Effect of light on the transformation of BDE-47 by living and autoclaved cultures of Microcystis flos-aquae and Chlorella vulgaris.

Polybrominated diphenyl ethers (PBDEs) are ubiquitous and toxic contaminants found in high concentrations in watercourses, and are not well removed by conventional wastewater treatment facilities. This study aimed to evaluate the removal and transformation of BDE-47, one of the environmentally predominant PBDE congener, by a green alga (Chlorella vulgaris) and a cyanobacterium (Microcystis flos-aquae) under different light conditions. Living and autoclaved cultures were exposed to BDE-47 at a concentration of 10 μg L for 7 days.

Growth and antioxidative response of two mangrove plants to interaction between aquaculture effluent and BDE-99.

Mangroves are subject to contamination of polybrominated diphenyl ethers (PBDEs) due to waste and wastewater disposal, and aquaculture effluent (AE) from nearby aquaculture activities. However, the response of mangrove plants to these two stresses and their interaction has seldom been reported. A six-month microcosm study, planted with either Kandelia obovata (Ko) or Avicennia marina (Am), the two most dominant species in South China mangrove swamps, was conducted to investigate the effects of BDE-99, and the interactions of BDE-99 (one of the most abundant PBDE congeners) and AE on growth and physiological responses of these plants.

Spatial distribution and source identification of hydrophobic organic compounds (HOCs) on sedimentary microplastic in Hong Kong.

The spatial distribution, composition and source of hydrophobic organic compounds (HOCs) including polyaromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organochlorinated pesticides (OCPs) of the sedimentary microplastics (0.25-5 mm) in Hong Kong were investigated. The concentration of ΣPAHs ranged between 70.